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dc.contributor.advisorVoolstra, Christian R.
dc.contributor.authorBaumgarten, Sebastian
dc.date.accessioned2013-07-11T05:25:17Z
dc.date.available2014-07-01T00:00:00Z
dc.date.issued2013-07
dc.identifier.citationBaumgarten, S. (2013). The MicroRNA Repertoire of Symbiodinium, the Dinoflagellate Symbiont of Reef-Building Corals. KAUST Research Repository. https://doi.org/10.25781/KAUST-A79NQ
dc.identifier.doi10.25781/KAUST-A79NQ
dc.identifier.urihttp://hdl.handle.net/10754/295746
dc.description.abstractAnimal and plant genomes produce numerous small RNAs (smRNAs) that regulate gene expression post-transcriptionally affecting metabolism, development, and epigenetic inheritance. In order to characterize the repertoire of endogenous microRNAs and potential gene targets, we conducted smRNA and mRNA expression profiling over nine experimental treatments of cultures from the dinoflagellate Symbiodinium sp. A1, a photosynthetic symbiont of scleractinian corals. We identified a total of 75 novel smRNAs in Symbiodinum sp. A1 that share stringent key features with functional microRNAs from other model organisms. A subset of 38 smRNAs was predicted independently over all nine treatments and their putative gene targets were identified. We found 3,187 animal-like target sites in the 3’UTRs of 12,858 mRNAs and 53 plantlike target sites in 51,917 genes. Furthermore, we identified the core RNAi protein machinery in Symbiodinium. Integration of smRNA and mRNA expression profiling identified a variety of processes that could be under microRNA control, e.g. regulation of translation, DNA modification, and chromatin silencing. Given that Symbiodinium seems to have a paucity of transcription factors and differentially expressed genes, identification and characterization of its smRNA repertoire establishes the possibility of a range of gene regulatory mechanisms in dinoflagellates acting post-transcriptionally.
dc.language.isoen
dc.subjectSymbiodinium
dc.subjectmicroRNA
dc.subjectsmall interfering RNA
dc.subjectscleractinian coral
dc.subjectdinoflagellate
dc.subjectsymbiont
dc.titleThe MicroRNA Repertoire of Symbiodinium, the Dinoflagellate Symbiont of Reef-Building Corals
dc.typeThesis
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.rights.embargodate2014-07-01
thesis.degree.grantorKing Abdullah University of Science and Technology
dc.contributor.committeememberAranda, Manuel
dc.contributor.committeememberPain, Arnab
thesis.degree.disciplineMarine Science
thesis.degree.nameMaster of Science
dc.rights.accessrightsAt the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2014-07-01.


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