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dc.contributor.advisorMerzaban, Jasmeen
dc.contributor.authorMahmood, Hanan
dc.date.accessioned2013-05-18T07:33:41Z
dc.date.available2013-05-18T07:33:41Z
dc.date.issued2013-05-18
dc.identifier.doi10.25781/KAUST-K4G00
dc.identifier.urihttp://hdl.handle.net/10754/292302
dc.description.abstractSuccessful bone marrow (BM) transplantation requires the homing of the transplanted hematopoietic stem/progenitor cells (HSPCs) to their bone marrow niche, where they undergo differentiation to form mature cells that are eventually released into the peripheral blood. However, the survival rate of patients receiving BM transplants is poor since many of the transplanted HSPCs do not make it to their BM niches in the recipient’s body. Since the availability of HSPCs from traditional sources is limited, transplanting more number of HSPCs is not a solution to this problem. This study aims to characterize the adhesion molecules mediating cell migration in order to better understand the adhesion mechanisms of HSCs with the bone marrow endothelium. This will aid in developing future tools to improve the clinical transplantation of HSPCs. This study also aims to understand the factors that influence HSPC proliferation in the bone marrow niche. E-selectin plays an important role in the process of homing; however, its ligands on HSPCs are not well characterized. We used western blotting and immunoprecipitation to show that endomucin is expressed on HSPCs and plays a role in the binding of HSPCs to E-selectin. We also studied the effect of recombinant E-selectin on the expression of a newly characterized E-selectin ligand in our lab, CD34, in HSPCs. This will provide us insight into novel roles for endomucin and E-selectin and help us to understand the factors influencing HSPC migration to BM endothelium.
dc.language.isoen
dc.subjectSelectin
dc.subjectEndomucin
dc.subjectHematopoietic Stem Cell
dc.subjectCD34
dc.titleCharacterization of Selectin Ligands on Hematopoietic Stem Cells
dc.typeThesis
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
thesis.degree.grantorKing Abdullah University of Science and Technology
dc.contributor.committeememberGadhoum, Samah Z.
dc.contributor.committeememberXiong, Liming
thesis.degree.disciplineBioscience
thesis.degree.nameMaster of Science
refterms.dateFOA2018-06-13T09:46:44Z


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