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    A Survey into Taxonomic and Physiological Differences of Symbiodinium sp., the Photosynthetic Symbiont of Reef-building Corals

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    XianzheGongThesis.pdf
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    Description:
    Xianzhe Gong - MS Thesis
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    Type
    Thesis
    Authors
    Gong, Xianzhe
    Advisors
    Voolstra, Christian R. cc
    Committee members
    Roder, Cornelia
    Stingl, Ulrich cc
    Program
    Marine Science
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Date
    2012-11
    Embargo End Date
    2013-07-28
    Permanent link to this record
    http://hdl.handle.net/10754/253670
    
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    Access Restrictions
    At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2013-07-28.
    Abstract
    The dinoflagellate genus Symbiodinium is a popular research topic in the coral reef molecular biology field. Primarily because these organisms serve as the coral holobiont’s primary source of energy, carrying out photosynthesis, and providing hydrocarbons to the coral host. Previous studies have shown the difficulty of isolating Symbiodinium as well as the inherent problems in trying to quantify the diversity of this genus and to qualify the distinct reactions of different Symbiodinium sp. to changing environmental conditions. The main goals of this study are: (1) to detail the relationship between the genetic classification of the organism and its physiology in regard to photosynthesis with a number of established Symbiodinium cultures; and (2) to isolate Symbiodinium from coral of the central Red Sea. To evaluate the photosynthetic physiology of Symbiodinium, a microsensor was used to measure oxygen concentrations along with a phytoplankton analyzer system that used pulse-amplitude-modulation (Phyto-PAM) to measure fluorescence. In order to identify the particular clade that the isolates belonged to, denaturing gradient gel electrophoresis (PCR-DGGE) was used to identify Symbiodinium based on their internal transcribed spacer 2 (ITS2) region. These techniques helped us to achieve our goals in the following ways: Symbiodinium sp. from a culture collection were classified to the subclade level; species-specific and clade-specific photosynthetic profiles were generated; and a Symbiodinium sp. was isolated from the central Red Sea. This study provided preliminary correlation between the photosynthetic difference and Symbiodinium genetic classification; showed the probable existence of a self-protection system inside the Symbiodinium cells by comparing the difference between the initial oxygen production at the beginning of each light step and the oxygen production after light adaptation; and confirmed the possibility of the isolation of Symbiodinium.
    Citation
    Gong, X. (2012). A Survey into Taxonomic and Physiological Differences of Symbiodinium sp., the Photosynthetic Symbiont of Reef-building Corals. KAUST Research Repository. https://doi.org/10.25781/KAUST-31281
    DOI
    10.25781/KAUST-31281
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-31281
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Marine Science Program; MS Theses

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