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    Spatial Exploration and Characterization of Endozoicomonas spp. Bacteria in Stylophora pistillata Using Fluorescence In Situ Hybridization

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    AreejAlsheikhHussainThesis.pdf
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    Type
    Thesis
    Authors
    Alsheikh-­Hussain, Areej
    Advisors
    Voolstra, Christian R. cc
    Committee members
    Apprill, Amy
    Stingl, Ulrich cc
    Program
    Marine Science
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2011-12-12
    Embargo End Date
    2012-12-31
    Permanent link to this record
    http://hdl.handle.net/10754/209406
    
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    Access Restrictions
    At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2012-12-31.
    Abstract
    Studies of coral-­associated bacterial communities have repeatedly demonstrated that the microbial assemblages of the coral host are highly specific and complex. In particular, bacterial community surveys of scleractinian and soft corals from geographically diverse reefs continually uncover a high abundance of sequences affiliated with the Gammaproteobacteria genus Endozoicomonas. The role of these bacteria within the complex coral holobiont is currently unknown. In order to localize these cells and gain an understanding of their potential interactions within the coral, we developed a fluorescence in situ hybridization(FISH) approach for reef-­building coral tissues. Using a custom small-­subunit ribosomal RNA gene database, we developed two Endozoicomonas-­specific probes that cover almost all known coral-­associated Endozoicomonas sequences. Probe hybridization conditions were quantitatively evaluated against target and non-­target bacterial cultures using fluorescence microscopy. Using these experimentally tested conditions, probes were then hybridized to the branching coral Stylophora pistillata, obtained from the Red Sea, using whole mount and paraffin embedding techniques. This study allowed preliminary spatial exploration and characterization of Endozoicomonas in coral, which has provided insight into their functional role and interactions within the coral holobiont.
    Citation
    Alsheikh-­Hussain, A. (2011). Spatial Exploration and Characterization of Endozoicomonas spp. Bacteria in Stylophora pistillata Using Fluorescence In Situ Hybridization. KAUST Research Repository. https://doi.org/10.25781/KAUST-RF4X8
    DOI
    10.25781/KAUST-RF4X8
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-RF4X8
    Scopus Count
    Collections
    Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program; Theses

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