Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions

Handle URI:
http://hdl.handle.net/10754/627760
Title:
Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions
Authors:
Scarascia, Giantommaso ( 0000-0003-3202-9871 ) ; Yap, Scott A.; Kaksonen, Anna H.; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
Pseudomonas aeruginosa is a ubiquitous member of marine biofilm, and reduces thiosulfate to produce toxic hydrogen sulfide gas. In this study, lytic bacteriophages were isolated and applied to inhibit the growth of P. aeruginosa in planktonic mode at different temperature, pH, and salinity. Bacteriophages showed optimal infectivity at a multiplicity of infection of 10 in saline conditions, and demonstrated lytic abilities over all tested temperature (25, 30, 37, and 45°C) and pH 6–9. Planktonic P. aeruginosa exhibited significantly longer lag phase and lower specific growth rates upon exposure to bacteriophages. Bacteriophages were subsequently applied to P. aeruginosa-enriched biofilm and were determined to lower the relative abundance of Pseudomonas-related taxa from 0.17 to 5.58% in controls to 0.01–0.61% in treated microbial communities. The relative abundance of Alphaproteobacteria, Pseudoalteromonas, and Planococcaceae decreased, possibly due to the phage-induced disruption of the biofilm matrix. Lastly, when applied to mitigate biofouling of ultrafiltration membranes, bacteriophages were determined to reduce the transmembrane pressure increase by 18% when utilized alone, and by 49% when used in combination with citric acid. The combined treatment was more effective compared with the citric acid treatment alone, which reported ca. 30% transmembrane pressure reduction. Collectively, the findings demonstrated that bacteriophages can be used as a biocidal agent to mitigate undesirable P. aeruginosa-associated problems in seawater applications.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC)
Citation:
Scarascia G, Yap SA, Kaksonen AH, Hong P-Y (2018) Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions. Frontiers in Microbiology 9. Available: http://dx.doi.org/10.3389/fmicb.2018.00875.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Microbiology
KAUST Grant Number:
URF/1/2982-01-01
Issue Date:
2-May-2018
DOI:
10.3389/fmicb.2018.00875
Type:
Article
ISSN:
1664-302X
Sponsors:
The research reported in this publication was supported by CRG funding URF/1/2982-01-01 from King Abdullah University of Science and Technology (KAUST) awarded to P-YH. AK thanks KAUST and CSIRO Land and Water for financial support.
Additional Links:
https://www.frontiersin.org/articles/10.3389/fmicb.2018.00875/
Appears in Collections:
Articles; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorScarascia, Giantommasoen
dc.contributor.authorYap, Scott A.en
dc.contributor.authorKaksonen, Anna H.en
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2018-05-07T07:36:24Z-
dc.date.available2018-05-07T07:36:24Z-
dc.date.issued2018-05-02en
dc.identifier.citationScarascia G, Yap SA, Kaksonen AH, Hong P-Y (2018) Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions. Frontiers in Microbiology 9. Available: http://dx.doi.org/10.3389/fmicb.2018.00875.en
dc.identifier.issn1664-302Xen
dc.identifier.doi10.3389/fmicb.2018.00875en
dc.identifier.urihttp://hdl.handle.net/10754/627760-
dc.description.abstractPseudomonas aeruginosa is a ubiquitous member of marine biofilm, and reduces thiosulfate to produce toxic hydrogen sulfide gas. In this study, lytic bacteriophages were isolated and applied to inhibit the growth of P. aeruginosa in planktonic mode at different temperature, pH, and salinity. Bacteriophages showed optimal infectivity at a multiplicity of infection of 10 in saline conditions, and demonstrated lytic abilities over all tested temperature (25, 30, 37, and 45°C) and pH 6–9. Planktonic P. aeruginosa exhibited significantly longer lag phase and lower specific growth rates upon exposure to bacteriophages. Bacteriophages were subsequently applied to P. aeruginosa-enriched biofilm and were determined to lower the relative abundance of Pseudomonas-related taxa from 0.17 to 5.58% in controls to 0.01–0.61% in treated microbial communities. The relative abundance of Alphaproteobacteria, Pseudoalteromonas, and Planococcaceae decreased, possibly due to the phage-induced disruption of the biofilm matrix. Lastly, when applied to mitigate biofouling of ultrafiltration membranes, bacteriophages were determined to reduce the transmembrane pressure increase by 18% when utilized alone, and by 49% when used in combination with citric acid. The combined treatment was more effective compared with the citric acid treatment alone, which reported ca. 30% transmembrane pressure reduction. Collectively, the findings demonstrated that bacteriophages can be used as a biocidal agent to mitigate undesirable P. aeruginosa-associated problems in seawater applications.en
dc.description.sponsorshipThe research reported in this publication was supported by CRG funding URF/1/2982-01-01 from King Abdullah University of Science and Technology (KAUST) awarded to P-YH. AK thanks KAUST and CSIRO Land and Water for financial support.en
dc.publisherFrontiers Media SAen
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2018.00875/en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectbacteriophageen
dc.subjectgreen biocidesen
dc.subjectbiofilm removalen
dc.subjectplanktonic cellsen
dc.subjectultrafiltration membraneen
dc.titleBacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditionsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalFrontiers in Microbiologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionLand and Water, Commonwealth Scientific and Industrial Research Organization, Canberra, ACT, Australiaen
kaust.authorScarascia, Giantommasoen
kaust.authorYap, Scott A.en
kaust.authorHong, Pei-Yingen
kaust.grant.numberURF/1/2982-01-01en
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