Patterns of Symbiodinium (Dinophyceae) diversity and assemblages among diverse hosts and the coral reef environment of Lizard Island, Australia

Handle URI:
http://hdl.handle.net/10754/627693
Title:
Patterns of Symbiodinium (Dinophyceae) diversity and assemblages among diverse hosts and the coral reef environment of Lizard Island, Australia
Authors:
Ziegler, Maren ( 0000-0003-2237-9261 ) ; Stone, Elizabeth; Colman, Daniel; Takacs-Vesbach, Cristina; Shepherd, Ursula
Abstract:
Large-scale environmental disturbances may impact both partners in coral host-Symbiodinium systems. Elucidation of the assembly patterns in such complex and interdependent communities may enable better prediction of environmental impacts across coral reef ecosystems. In this study, we investigated how the community composition and diversity of dinoflagellate symbionts in the genus Symbiodinium were distributed among 12 host species from six taxonomic orders (Actinaria, Alcyonacea, Miliolida, Porifera, Rhizostoma, Scleractinia) and in the reef water and sediments at Lizard Island, Great Barrier Reef before the 3rd Global Coral Bleaching Event. 454 pyrosequencing of the ITS2 region of Symbiodinium yielded 83 Operational Taxonomic Units (OTUs) at a 97% similarity cut-off. Approximately half of the Symbiodinium OTUs from reef water or sediments were also present in symbio. OTUs belonged to six clades (A-D, F-G), but community structure was uneven. The two most abundant OTUs (100% matches to types C1 and A3) comprised 91% of reads and OTU C1 was shared by all species. However, sequence-based analysis of these dominant OTUs revealed host species-specificity, suggesting that genetic similarity cut-offs of Symbiodinium ITS2 data sets need careful evaluation. Of the less abundant OTUs, roughly half occurred at only one site or in one species and the background Symbiodinium communities were distinct between individual samples. We conclude that sampling multiple host taxa with differing life history traits will be critical to fully understand the symbiont diversity of a given system and to predict coral ecosystem responses to environmental change and disturbance considering the differential stress response of the taxa within. This article is protected by copyright. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Ziegler M, Stone E, Colman D, Takacs-Vesbach C, Shepherd U (2018) Patterns of Symbiodinium (Dinophyceae) diversity and assemblages among diverse hosts and the coral reef environment of Lizard Island, Australia. Journal of Phycology. Available: http://dx.doi.org/10.1111/jpy.12749.
Publisher:
Wiley
Journal:
Journal of Phycology
Issue Date:
26-Apr-2018
DOI:
10.1111/jpy.12749
Type:
Article
ISSN:
0022-3646
Sponsors:
We are grateful to Lizard Island Research Station staff for their support. Samples were collected under Great Barrier Reef Marine Parks permit # G13/36006.1. The trip to Lizard Island and the molecular work were funded by the UNM Honors College Research Institute, Sonnet and Ian McKinnon and by a UNM Research Allocations Grant. Research reported in this publication was supported by the National Institute of General medical Sciences of the National Institutes of Health under Award Number P30GM110907. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We thank the UNM undergraduate course participants for assistance with sample collection and the reviewers and editor for their comments that helped to improve this manuscript.
Additional Links:
https://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.12749
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZiegler, Marenen
dc.contributor.authorStone, Elizabethen
dc.contributor.authorColman, Danielen
dc.contributor.authorTakacs-Vesbach, Cristinaen
dc.contributor.authorShepherd, Ursulaen
dc.date.accessioned2018-04-30T06:58:23Z-
dc.date.available2018-04-30T06:58:23Z-
dc.date.issued2018-04-26en
dc.identifier.citationZiegler M, Stone E, Colman D, Takacs-Vesbach C, Shepherd U (2018) Patterns of Symbiodinium (Dinophyceae) diversity and assemblages among diverse hosts and the coral reef environment of Lizard Island, Australia. Journal of Phycology. Available: http://dx.doi.org/10.1111/jpy.12749.en
dc.identifier.issn0022-3646en
dc.identifier.doi10.1111/jpy.12749en
dc.identifier.urihttp://hdl.handle.net/10754/627693-
dc.description.abstractLarge-scale environmental disturbances may impact both partners in coral host-Symbiodinium systems. Elucidation of the assembly patterns in such complex and interdependent communities may enable better prediction of environmental impacts across coral reef ecosystems. In this study, we investigated how the community composition and diversity of dinoflagellate symbionts in the genus Symbiodinium were distributed among 12 host species from six taxonomic orders (Actinaria, Alcyonacea, Miliolida, Porifera, Rhizostoma, Scleractinia) and in the reef water and sediments at Lizard Island, Great Barrier Reef before the 3rd Global Coral Bleaching Event. 454 pyrosequencing of the ITS2 region of Symbiodinium yielded 83 Operational Taxonomic Units (OTUs) at a 97% similarity cut-off. Approximately half of the Symbiodinium OTUs from reef water or sediments were also present in symbio. OTUs belonged to six clades (A-D, F-G), but community structure was uneven. The two most abundant OTUs (100% matches to types C1 and A3) comprised 91% of reads and OTU C1 was shared by all species. However, sequence-based analysis of these dominant OTUs revealed host species-specificity, suggesting that genetic similarity cut-offs of Symbiodinium ITS2 data sets need careful evaluation. Of the less abundant OTUs, roughly half occurred at only one site or in one species and the background Symbiodinium communities were distinct between individual samples. We conclude that sampling multiple host taxa with differing life history traits will be critical to fully understand the symbiont diversity of a given system and to predict coral ecosystem responses to environmental change and disturbance considering the differential stress response of the taxa within. This article is protected by copyright. All rights reserved.en
dc.description.sponsorshipWe are grateful to Lizard Island Research Station staff for their support. Samples were collected under Great Barrier Reef Marine Parks permit # G13/36006.1. The trip to Lizard Island and the molecular work were funded by the UNM Honors College Research Institute, Sonnet and Ian McKinnon and by a UNM Research Allocations Grant. Research reported in this publication was supported by the National Institute of General medical Sciences of the National Institutes of Health under Award Number P30GM110907. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We thank the UNM undergraduate course participants for assistance with sample collection and the reviewers and editor for their comments that helped to improve this manuscript.en
dc.publisherWileyen
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.12749en
dc.rightsArchived with thanks to Journal of Phycologyen
dc.subjectForaminiferaen
dc.subjectCoral reefen
dc.subjectScleractiniaen
dc.subjectTridacnaen
dc.subject454 Pyrosequencingen
dc.subjectAlcyonaceaen
dc.subjectSymbiont Diversityen
dc.subjectActinariaen
dc.titlePatterns of Symbiodinium (Dinophyceae) diversity and assemblages among diverse hosts and the coral reef environment of Lizard Island, Australiaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalJournal of Phycologyen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Biology; University of New Mexico, Castetter Hall, MSC03-2020 1 University of New Mexico; Albuquerque New Mexico 87131 USAen
dc.contributor.institutionHonors College, University of New Mexico, Student Health Center Building, MSCO6-3890 1 University of New Mexico; Albuquerque New Mexico 87131 USAen
kaust.authorZiegler, Marenen
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