Effect of Salt on the Metabolism of ‘Candidatus Accumulibacter’ Clade I and II

Handle URI:
http://hdl.handle.net/10754/627386
Title:
Effect of Salt on the Metabolism of ‘Candidatus Accumulibacter’ Clade I and II
Authors:
Wang, Zhongwei ( 0000-0002-1476-5093 ) ; Dunne, Aislinn; van Loosdrecht, Mark C. M.; Saikaly, Pascal ( 0000-0001-7678-3986 )
Abstract:
Saline wastewater is known to affect the performance of phosphate-accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) process. However, studies comparing the effect of salinity on different PAO clades are lacking. In this study, 'Candidatus Accumulibacter phosphatis' Clade I and II (hereafter referred to as PAOI and PAOII) were highly enriched (~90% in relative abundance as determined by quantitative FISH) in the form of granules in two sequencing batch reactors. Anaerobic and aerobic batch experiments were conducted to evaluate the effect of salinity on the kinetics and stoichiometry of PAOI and PAOII. PAOI and PAOII communities showed different priority in using polyphosphate (poly-P) and glycogen to generate ATP in the anaerobic phase when exposed to salt, with PAOI depending more on intracellular poly-P degradation (e.g., the proportion of calculated ATP derived from poly-P increased by 5-6% at 0.256 mol/L NaCl or KCl) while PAOII on glycolysis of intracellularly stored glycogen (e.g., the proportion of calculated ATP derived from glycogen increased by 29-30% at 0.256 mol/L NaCl or KCl). In the aerobic phase, the loss of phosphate uptake capability was more pronounced in PAOII due to the higher energy cost to synthesize their larger glycogen pool compared to PAOI. For both PAOI and PAOII, aerobic conversion rates were more sensitive to salt than anaerobic conversion rates. Potassium (K) and sodium (Na) ions exhibited different effect regardless of the enriched PAO culture, suggesting that the composition of salt is an important factor to consider when studying the effect of salt on EBPR performance.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC)
Citation:
Wang Z, Dunne A, van Loosdrecht MCM, Saikaly PE (2018) Effect of Salt on the Metabolism of “Candidatus Accumulibacter” Clade I and II. Frontiers in Microbiology 9. Available: http://dx.doi.org/10.3389/fmicb.2018.00479.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Microbiology
Issue Date:
16-Mar-2018
DOI:
10.3389/fmicb.2018.00479
Type:
Article
ISSN:
1664-302X
Sponsors:
The authors would like to thank Dr. Laurens Welles at UNESCO-IHE Institute for Water Education for providing PAOI culture.This work was supported by King Abdullah University of Science and Technology (KAUST).
Additional Links:
https://www.frontiersin.org/articles/10.3389/fmicb.2018.00479/full
Appears in Collections:
Articles; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Zhongweien
dc.contributor.authorDunne, Aislinnen
dc.contributor.authorvan Loosdrecht, Mark C. M.en
dc.contributor.authorSaikaly, Pascalen
dc.date.accessioned2018-04-01T08:20:27Z-
dc.date.available2018-04-01T08:20:27Z-
dc.date.issued2018-03-16en
dc.identifier.citationWang Z, Dunne A, van Loosdrecht MCM, Saikaly PE (2018) Effect of Salt on the Metabolism of “Candidatus Accumulibacter” Clade I and II. Frontiers in Microbiology 9. Available: http://dx.doi.org/10.3389/fmicb.2018.00479.en
dc.identifier.issn1664-302Xen
dc.identifier.doi10.3389/fmicb.2018.00479en
dc.identifier.urihttp://hdl.handle.net/10754/627386-
dc.description.abstractSaline wastewater is known to affect the performance of phosphate-accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) process. However, studies comparing the effect of salinity on different PAO clades are lacking. In this study, 'Candidatus Accumulibacter phosphatis' Clade I and II (hereafter referred to as PAOI and PAOII) were highly enriched (~90% in relative abundance as determined by quantitative FISH) in the form of granules in two sequencing batch reactors. Anaerobic and aerobic batch experiments were conducted to evaluate the effect of salinity on the kinetics and stoichiometry of PAOI and PAOII. PAOI and PAOII communities showed different priority in using polyphosphate (poly-P) and glycogen to generate ATP in the anaerobic phase when exposed to salt, with PAOI depending more on intracellular poly-P degradation (e.g., the proportion of calculated ATP derived from poly-P increased by 5-6% at 0.256 mol/L NaCl or KCl) while PAOII on glycolysis of intracellularly stored glycogen (e.g., the proportion of calculated ATP derived from glycogen increased by 29-30% at 0.256 mol/L NaCl or KCl). In the aerobic phase, the loss of phosphate uptake capability was more pronounced in PAOII due to the higher energy cost to synthesize their larger glycogen pool compared to PAOI. For both PAOI and PAOII, aerobic conversion rates were more sensitive to salt than anaerobic conversion rates. Potassium (K) and sodium (Na) ions exhibited different effect regardless of the enriched PAO culture, suggesting that the composition of salt is an important factor to consider when studying the effect of salt on EBPR performance.en
dc.description.sponsorshipThe authors would like to thank Dr. Laurens Welles at UNESCO-IHE Institute for Water Education for providing PAOI culture.This work was supported by King Abdullah University of Science and Technology (KAUST).en
dc.publisherFrontiers Media SAen
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2018.00479/fullen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject'Candidatus Accumulibacter phosphatis' clade I and IIen
dc.subjectEnhanced biological phosphorus removalen
dc.subjectPhosphate-accumulating organism (PAO)en
dc.subjectSaline wastewateren
dc.subjectWater scarcityen
dc.titleEffect of Salt on the Metabolism of ‘Candidatus Accumulibacter’ Clade I and IIen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalFrontiers in Microbiologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Biotechnology, Faculty of Applied Sciences, Delft University of Technology, Delft, , Netherlandsen
kaust.authorWang, Zhongweien
kaust.authorDunne, Aislinnen
kaust.authorSaikaly, Pascalen
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