Using Aiptasia as a Model to Study Metabolic Interactions in Cnidarian-Symbiodinium Symbioses

Handle URI:
http://hdl.handle.net/10754/627369
Title:
Using Aiptasia as a Model to Study Metabolic Interactions in Cnidarian-Symbiodinium Symbioses
Authors:
Radecker, Nils; Raina, Jean-Baptiste; Pernice, Mathieu; Perna, Gabriela; Guagliardo, Paul; Kilburn, Matt R.; Aranda, Manuel ( 0000-0001-6673-016X ) ; Voolstra, Christian R. ( 0000-0003-4555-3795 )
Abstract:
The symbiosis between cnidarian hosts and microalgae of the genus Symbiodinium provides the foundation of coral reefs in oligotrophic waters. Understanding the nutrient-exchange between these partners is key to identifying the fundamental mechanisms behind this symbiosis, yet has proven difficult given the endosymbiotic nature of this relationship. In this study, we investigated the respective contribution of host and symbiont to carbon and nitrogen assimilation in the coral model anemone Aiptaisa. For this, we combined traditional measurements with nanoscale secondary ion mass spectrometry (NanoSIMS) and stable isotope labeling to investigate patterns of nutrient uptake and translocation both at the organismal scale and at the cellular scale. Our results show that the rate of carbon and nitrogen assimilation in Aiptasia depends on the identity of the host and the symbiont. NanoSIMS analysis confirmed that both host and symbiont incorporated carbon and nitrogen into their cells, implying a rapid uptake and cycling of nutrients in this symbiotic relationship. Gross carbon fixation was highest in Aiptasia associated with their native Symbiodinium communities. However, differences in fixation rates were only reflected in the δ13C enrichment of the cnidarian host, whereas the algal symbiont showed stable enrichment levels regardless of host identity. Thereby, our results point toward a “selfish” character of the cnidarian—Symbiodinium association in which both partners directly compete for available resources. Consequently, this symbiosis may be inherently instable and highly susceptible to environmental change. While questions remain regarding the underlying cellular controls of nutrient exchange and the nature of metabolites involved, the approach outlined in this study constitutes a powerful toolset to address these questions.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program; Red Sea Research Center (RSRC)
Citation:
Rädecker N, Raina J-B, Pernice M, Perna G, Guagliardo P, et al. (2018) Using Aiptasia as a Model to Study Metabolic Interactions in Cnidarian-Symbiodinium Symbioses. Frontiers in Physiology 9. Available: http://dx.doi.org/10.3389/fphys.2018.00214.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Physiology
Issue Date:
16-Mar-2018
DOI:
10.3389/fphys.2018.00214
Type:
Article
ISSN:
1664-042X
Sponsors:
The authors would like to thank Dr. Rachid Sougrat and Ptissam Bergam from the KAUST imaging core lab for their help with sample preparation. CV and NR acknowledge funding from the KAUST CPF funding. Further, research in this publication was supported by KAUST baseline research funds to CV. The authors would like to acknowledge the Australian Microscopy & Microanalysis Research Facility, AuScope, the Science and Industry Endowment Fund, and the State Government of Western Australian for contributing to the Ion Probe Facility at the Centre for Microscopy, Characterisation and Analysis at the University of Western Australia. J-BR was supported by Australian Research Council fellowship DE160100636.
Additional Links:
https://www.frontiersin.org/articles/10.3389/fphys.2018.00214/full#h6
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Marine Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorRadecker, Nilsen
dc.contributor.authorRaina, Jean-Baptisteen
dc.contributor.authorPernice, Mathieuen
dc.contributor.authorPerna, Gabrielaen
dc.contributor.authorGuagliardo, Paulen
dc.contributor.authorKilburn, Matt R.en
dc.contributor.authorAranda, Manuelen
dc.contributor.authorVoolstra, Christian R.en
dc.date.accessioned2018-03-20T12:34:07Z-
dc.date.available2018-03-20T12:34:07Z-
dc.date.issued2018-03-16en
dc.identifier.citationRädecker N, Raina J-B, Pernice M, Perna G, Guagliardo P, et al. (2018) Using Aiptasia as a Model to Study Metabolic Interactions in Cnidarian-Symbiodinium Symbioses. Frontiers in Physiology 9. Available: http://dx.doi.org/10.3389/fphys.2018.00214.en
dc.identifier.issn1664-042Xen
dc.identifier.doi10.3389/fphys.2018.00214en
dc.identifier.urihttp://hdl.handle.net/10754/627369-
dc.description.abstractThe symbiosis between cnidarian hosts and microalgae of the genus Symbiodinium provides the foundation of coral reefs in oligotrophic waters. Understanding the nutrient-exchange between these partners is key to identifying the fundamental mechanisms behind this symbiosis, yet has proven difficult given the endosymbiotic nature of this relationship. In this study, we investigated the respective contribution of host and symbiont to carbon and nitrogen assimilation in the coral model anemone Aiptaisa. For this, we combined traditional measurements with nanoscale secondary ion mass spectrometry (NanoSIMS) and stable isotope labeling to investigate patterns of nutrient uptake and translocation both at the organismal scale and at the cellular scale. Our results show that the rate of carbon and nitrogen assimilation in Aiptasia depends on the identity of the host and the symbiont. NanoSIMS analysis confirmed that both host and symbiont incorporated carbon and nitrogen into their cells, implying a rapid uptake and cycling of nutrients in this symbiotic relationship. Gross carbon fixation was highest in Aiptasia associated with their native Symbiodinium communities. However, differences in fixation rates were only reflected in the δ13C enrichment of the cnidarian host, whereas the algal symbiont showed stable enrichment levels regardless of host identity. Thereby, our results point toward a “selfish” character of the cnidarian—Symbiodinium association in which both partners directly compete for available resources. Consequently, this symbiosis may be inherently instable and highly susceptible to environmental change. While questions remain regarding the underlying cellular controls of nutrient exchange and the nature of metabolites involved, the approach outlined in this study constitutes a powerful toolset to address these questions.en
dc.description.sponsorshipThe authors would like to thank Dr. Rachid Sougrat and Ptissam Bergam from the KAUST imaging core lab for their help with sample preparation. CV and NR acknowledge funding from the KAUST CPF funding. Further, research in this publication was supported by KAUST baseline research funds to CV. The authors would like to acknowledge the Australian Microscopy & Microanalysis Research Facility, AuScope, the Science and Industry Endowment Fund, and the State Government of Western Australian for contributing to the Ion Probe Facility at the Centre for Microscopy, Characterisation and Analysis at the University of Western Australia. J-BR was supported by Australian Research Council fellowship DE160100636.en
dc.publisherFrontiers Media SAen
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fphys.2018.00214/full#h6en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleUsing Aiptasia as a Model to Study Metabolic Interactions in Cnidarian-Symbiodinium Symbiosesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMarine Science Programen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalFrontiers in Physiologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionClimate Change Cluster, University of Technology Sydney, Sydney, NSW, Australiaen
dc.contributor.institutionCentre for Microscopy, Characterisation and Analysis, University of Western Australia, Perth, WA, Australiaen
kaust.authorRadecker, Nilsen
kaust.authorPerna, Gabrielaen
kaust.authorAranda, Manuelen
kaust.authorVoolstra, Christian R.en
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