Phototoxic effects of PAH and UVA exposure on molecular responses and developmental success in coral larvae

Handle URI:
http://hdl.handle.net/10754/627334
Title:
Phototoxic effects of PAH and UVA exposure on molecular responses and developmental success in coral larvae
Authors:
Overmans, Sebastian ( 0000-0003-3462-6629 ) ; Nordborg, Mikaela ( 0000-0002-9254-0367 ) ; Rua, Ruben Diaz; Brinkman, Diane L.; Negri, Andrew P.; Agusti, Susana ( 0000-0003-0536-7293 )
Abstract:
Exposure to polycyclic aromatic carbons (PAHs) poses a growing risk to coral reefs due to increasing shipping and petroleum extraction in tropical waters. Damaging effects of specific PAHs can be further enhanced by the presence of ultraviolet radiation, known as phototoxicity. We tested phototoxic effects of the PAHs anthracene and phenanthrene on larvae of the scleractinian coral Acropora tenuis in the presence and absence of UVA (320–400 nm). Activity of superoxide dismutase (SOD) enzyme was reduced by anthracene while phenanthrene and UVA exposure did not have any effect. Gene expression of MnSod remained constant across all treatments. The genes Catalase, Hsp70 and Hsp90 showed increased expression levels in larvae exposed to anthracene, but not phenanthrene. Gene expression of p53 was upregulated in the presence of UVA, but downregulated when exposed to PAHs. The influence on stress-related biochemical pathways and gene expresson in A. tenuis larvae was considerably greater for anthracene than phenanthrene, and UVA-induced phototoxicity was only evident for anthracene. The combined effects of UVA and PAH exposure on larval survival and metamorphosis paralleled the sub-lethal stress responses, clearly highlighting the interaction of UVA on anthracene toxicity and ultimately the coral’s development.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program; Red Sea Research Center (RSRC)
Citation:
Overmans S, Nordborg M, Rua RD, Brinkman DL, Negri AP, et al. (2018) Phototoxic effects of PAH and UVA exposure on molecular responses and developmental success in coral larvae. Aquatic Toxicology. Available: http://dx.doi.org/10.1016/j.aquatox.2018.03.008.
Publisher:
Elsevier BV
Journal:
Aquatic Toxicology
Issue Date:
9-Mar-2018
DOI:
10.1016/j.aquatox.2018.03.008
Type:
Article
ISSN:
0166-445X
Sponsors:
We would like to express special thanks to Florita Flores and the SeaSim staff from AIMS for their expert support and assistance. This work was made possible by the KAUST baseline funds to S. Agustí, and a research grant set up as part of the collaborative research project between the Red Sea Research Center (RSRC) at King Abdullah University of Science and Technology (KAUST) and the Australian Institute of Marine Science (AIMS).
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0166445X18302364
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Marine Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorOvermans, Sebastianen
dc.contributor.authorNordborg, Mikaelaen
dc.contributor.authorRua, Ruben Diazen
dc.contributor.authorBrinkman, Diane L.en
dc.contributor.authorNegri, Andrew P.en
dc.contributor.authorAgusti, Susanaen
dc.date.accessioned2018-03-15T11:35:53Z-
dc.date.available2018-03-15T11:35:53Z-
dc.date.issued2018-03-09en
dc.identifier.citationOvermans S, Nordborg M, Rua RD, Brinkman DL, Negri AP, et al. (2018) Phototoxic effects of PAH and UVA exposure on molecular responses and developmental success in coral larvae. Aquatic Toxicology. Available: http://dx.doi.org/10.1016/j.aquatox.2018.03.008.en
dc.identifier.issn0166-445Xen
dc.identifier.doi10.1016/j.aquatox.2018.03.008en
dc.identifier.urihttp://hdl.handle.net/10754/627334-
dc.description.abstractExposure to polycyclic aromatic carbons (PAHs) poses a growing risk to coral reefs due to increasing shipping and petroleum extraction in tropical waters. Damaging effects of specific PAHs can be further enhanced by the presence of ultraviolet radiation, known as phototoxicity. We tested phototoxic effects of the PAHs anthracene and phenanthrene on larvae of the scleractinian coral Acropora tenuis in the presence and absence of UVA (320–400 nm). Activity of superoxide dismutase (SOD) enzyme was reduced by anthracene while phenanthrene and UVA exposure did not have any effect. Gene expression of MnSod remained constant across all treatments. The genes Catalase, Hsp70 and Hsp90 showed increased expression levels in larvae exposed to anthracene, but not phenanthrene. Gene expression of p53 was upregulated in the presence of UVA, but downregulated when exposed to PAHs. The influence on stress-related biochemical pathways and gene expresson in A. tenuis larvae was considerably greater for anthracene than phenanthrene, and UVA-induced phototoxicity was only evident for anthracene. The combined effects of UVA and PAH exposure on larval survival and metamorphosis paralleled the sub-lethal stress responses, clearly highlighting the interaction of UVA on anthracene toxicity and ultimately the coral’s development.en
dc.description.sponsorshipWe would like to express special thanks to Florita Flores and the SeaSim staff from AIMS for their expert support and assistance. This work was made possible by the KAUST baseline funds to S. Agustí, and a research grant set up as part of the collaborative research project between the Red Sea Research Center (RSRC) at King Abdullah University of Science and Technology (KAUST) and the Australian Institute of Marine Science (AIMS).en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0166445X18302364en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Aquatic Toxicology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquatic Toxicology, [, , (2018-03-09)] DOI: 10.1016/j.aquatox.2018.03.008 . © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectCoralen
dc.subjectAcroporaen
dc.subjectPolycyclic aromatic hydrocarbon (PAH)en
dc.subjectUV radiationen
dc.subjectPhototoxicityen
dc.subjectGene expressionen
dc.subjectSuperoxide dismutase (SOD)en
dc.titlePhototoxic effects of PAH and UVA exposure on molecular responses and developmental success in coral larvaeen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMarine Science Programen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalAquatic Toxicologyen
dc.eprint.versionPost-printen
dc.contributor.institutionAustralian Institute of Marine Science (AIMS), Townsville, 4810, Queensland, Australiaen
kaust.authorOvermans, Sebastianen
kaust.authorRua, Ruben Diazen
kaust.authorAgusti, Susanaen
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