High-Throughput Sequencing and Linkage Mapping of a Clownfish Genome Provide Insights on the Distribution of Molecular Players Involved in Sex Change

Handle URI:
http://hdl.handle.net/10754/627329
Title:
High-Throughput Sequencing and Linkage Mapping of a Clownfish Genome Provide Insights on the Distribution of Molecular Players Involved in Sex Change
Authors:
Casas, Laura ( 0000-0001-6617-8731 ) ; Saenz Agudelo, Pablo ( 0000-0001-8197-2861 ) ; Irigoien, Xabier ( 0000-0002-5411-6741 )
Abstract:
Clownfishes are an excellent model system for investigating the genetic mechanism governing hermaphroditism and socially-controlled sex change in their natural environment because they are broadly distributed and strongly site-attached. Genomic tools, such as genetic linkage maps, allow fine-mapping of loci involved in molecular pathways underlying these reproductive processes. In this study, a high-density genetic map of Amphiprion bicinctus was constructed with 3146 RAD markers in a full-sib family organized in 24 robust linkage groups which correspond to the haploid chromosome number of the species. The length of the map was 4294.71 cM, with an average marker interval of 1.38 cM. The clownfish linkage map showed various levels of conserved synteny and collinearity with the genomes of Asian and European seabass, Nile tilapia and stickleback. The map provided a platform to investigate the genomic position of genes with differential expression during sex change in A. bicinctus. This study aims to bridge the gap of genome-scale information for this iconic group of species to facilitate the study of the main gene regulatory networks governing social sex change and gonadal restructuring in protandrous hermaphrodites.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Casas L, Saenz-Agudelo P, Irigoien X (2018) High-Throughput Sequencing and Linkage Mapping of a Clownfish Genome Provide Insights on the Distribution of Molecular Players Involved in Sex Change. Scientific Reports 8. Available: http://dx.doi.org/10.1038/s41598-018-22282-0.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
28-Feb-2018
DOI:
10.1038/s41598-018-22282-0
Type:
Article
ISSN:
2045-2322
Sponsors:
The authors would like to thank the Coastal and Marine Resources Core Lab, particularly Dave H. Pallet, for their invaluable support during fieldwork. We would like to express our gratitude to Konrad Paszkiewicz and Julian Catchen for technical advice, Craig T. Michell for technical assistance and the anonymous reviewers for their constructive comments on the manuscript. The clownfish drawing depicted in Fig. 3 is courtesy of Ivan Gromicho (Academic Writing Services, KAUST, Thuwal, Saudi Arabia).
Additional Links:
https://www.nature.com/articles/s41598-018-22282-0
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorCasas, Lauraen
dc.contributor.authorSaenz Agudelo, Pabloen
dc.contributor.authorIrigoien, Xabieren
dc.date.accessioned2018-03-15T11:35:53Z-
dc.date.available2018-03-15T11:35:53Z-
dc.date.issued2018-02-28en
dc.identifier.citationCasas L, Saenz-Agudelo P, Irigoien X (2018) High-Throughput Sequencing and Linkage Mapping of a Clownfish Genome Provide Insights on the Distribution of Molecular Players Involved in Sex Change. Scientific Reports 8. Available: http://dx.doi.org/10.1038/s41598-018-22282-0.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/s41598-018-22282-0en
dc.identifier.urihttp://hdl.handle.net/10754/627329-
dc.description.abstractClownfishes are an excellent model system for investigating the genetic mechanism governing hermaphroditism and socially-controlled sex change in their natural environment because they are broadly distributed and strongly site-attached. Genomic tools, such as genetic linkage maps, allow fine-mapping of loci involved in molecular pathways underlying these reproductive processes. In this study, a high-density genetic map of Amphiprion bicinctus was constructed with 3146 RAD markers in a full-sib family organized in 24 robust linkage groups which correspond to the haploid chromosome number of the species. The length of the map was 4294.71 cM, with an average marker interval of 1.38 cM. The clownfish linkage map showed various levels of conserved synteny and collinearity with the genomes of Asian and European seabass, Nile tilapia and stickleback. The map provided a platform to investigate the genomic position of genes with differential expression during sex change in A. bicinctus. This study aims to bridge the gap of genome-scale information for this iconic group of species to facilitate the study of the main gene regulatory networks governing social sex change and gonadal restructuring in protandrous hermaphrodites.en
dc.description.sponsorshipThe authors would like to thank the Coastal and Marine Resources Core Lab, particularly Dave H. Pallet, for their invaluable support during fieldwork. We would like to express our gratitude to Konrad Paszkiewicz and Julian Catchen for technical advice, Craig T. Michell for technical assistance and the anonymous reviewers for their constructive comments on the manuscript. The clownfish drawing depicted in Fig. 3 is courtesy of Ivan Gromicho (Academic Writing Services, KAUST, Thuwal, Saudi Arabia).en
dc.publisherSpringer Natureen
dc.relation.urlhttps://www.nature.com/articles/s41598-018-22282-0en
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleHigh-Throughput Sequencing and Linkage Mapping of a Clownfish Genome Provide Insights on the Distribution of Molecular Players Involved in Sex Changeen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionIKERBASQUE, Basque Foundation for Science, Bilbao, Spainen
dc.contributor.institutionAZTI - Marine Research, Herrera Kaia, Portualdea z/g –, 20110, Pasaia, Gipuzkoa, Spainen
kaust.authorCasas, Lauraen
kaust.authorSaenz Agudelo, Pabloen
kaust.authorIrigoien, Xabieren
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