Evaluation of two autoinducer-2 quantification methods for application in marine environments

Handle URI:
http://hdl.handle.net/10754/627131
Title:
Evaluation of two autoinducer-2 quantification methods for application in marine environments
Authors:
Wang, Tian-Nyu; Kaksonen, Anna H.; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
This study evaluated two methods, namely high performance liquid chromatography with fluorescence detection (HPLC-FLD) and Vibrio harveyi BB170 bioassay, for autoinducer-2 (AI-2) quantification in marine samples. Using both methods, the study also investigated the stability of AI-2 in varying pH, temperature and media, as well as quantified the amount of AI-2 signals in marine samples.HPLC-FLD method showed a higher level of reproducibility and precision compared to V. harveyi BB170 bioassay. Alkaline pH > 8 and high temperature (> 37°C) increased the instability of AI-2. The AI-2 concentrations in seawater were low, ca. 3.2-27.6 pmol l-1 whereas 8- week old marine biofilm grew on an 18.8 cm2 substratum accumulated ca. 0.207 nmol of AI-2.Both methods have pros and cons for AI-2 quantification in marine samples. Regardless, both methods reported a ubiquitous presence of AI-2 in both planktonic and biomass fractions of seawater, as well as in marine biofilm.In this study, AI-2 signals were for the first time enumerated in marine samples to reveal the ubiquitous presence of AI-2 in this environment. The findings suggest a possible role of AI-2 in biofilm formation in marine environment, and the contribution of AI-2 in biofilm-associated problems such as biofouling and biocorrosion. This article is protected by copyright. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC)
Citation:
Wang T-N, Kaksonen AH, Hong P-Y (2018) Evaluation of two autoinducer-2 quantification methods for application in marine environments. Journal of Applied Microbiology. Available: http://dx.doi.org/10.1111/jam.13725.
Publisher:
Wiley-Blackwell
Journal:
Journal of Applied Microbiology
KAUST Grant Number:
URF/1/2982-01-01
Issue Date:
11-Feb-2018
DOI:
10.1111/jam.13725
Type:
Article
ISSN:
1364-5072
Sponsors:
The work was funded by King Abdullah University of Science and Technology (KAUST) Competitive Research Grant 2017 (URF/1/2982-01-01) awarded to Pei-Ying Hong. CSIRO Land and Water is thanked for support to Anna Kaksonen.
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/jam.13725/abstract
Appears in Collections:
Articles; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Tian-Nyuen
dc.contributor.authorKaksonen, Anna H.en
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2018-02-14T11:52:53Z-
dc.date.available2018-02-14T11:52:53Z-
dc.date.issued2018-02-11en
dc.identifier.citationWang T-N, Kaksonen AH, Hong P-Y (2018) Evaluation of two autoinducer-2 quantification methods for application in marine environments. Journal of Applied Microbiology. Available: http://dx.doi.org/10.1111/jam.13725.en
dc.identifier.issn1364-5072en
dc.identifier.doi10.1111/jam.13725en
dc.identifier.urihttp://hdl.handle.net/10754/627131-
dc.description.abstractThis study evaluated two methods, namely high performance liquid chromatography with fluorescence detection (HPLC-FLD) and Vibrio harveyi BB170 bioassay, for autoinducer-2 (AI-2) quantification in marine samples. Using both methods, the study also investigated the stability of AI-2 in varying pH, temperature and media, as well as quantified the amount of AI-2 signals in marine samples.HPLC-FLD method showed a higher level of reproducibility and precision compared to V. harveyi BB170 bioassay. Alkaline pH > 8 and high temperature (> 37°C) increased the instability of AI-2. The AI-2 concentrations in seawater were low, ca. 3.2-27.6 pmol l-1 whereas 8- week old marine biofilm grew on an 18.8 cm2 substratum accumulated ca. 0.207 nmol of AI-2.Both methods have pros and cons for AI-2 quantification in marine samples. Regardless, both methods reported a ubiquitous presence of AI-2 in both planktonic and biomass fractions of seawater, as well as in marine biofilm.In this study, AI-2 signals were for the first time enumerated in marine samples to reveal the ubiquitous presence of AI-2 in this environment. The findings suggest a possible role of AI-2 in biofilm formation in marine environment, and the contribution of AI-2 in biofilm-associated problems such as biofouling and biocorrosion. This article is protected by copyright. All rights reserved.en
dc.description.sponsorshipThe work was funded by King Abdullah University of Science and Technology (KAUST) Competitive Research Grant 2017 (URF/1/2982-01-01) awarded to Pei-Ying Hong. CSIRO Land and Water is thanked for support to Anna Kaksonen.en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/jam.13725/abstracten
dc.rightsThis is the peer reviewed version of the following article: Evaluation of two autoinducer-2 quantification methods for application in marine environments, which has been published in final form at http://doi.org/10.1111/jam.13725. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.subjectBiofoulingen
dc.subjectAutoinducer-2en
dc.subjectMarine Biofilmen
dc.subject4,5-Dihydroxy-2,3-pentandioneen
dc.titleEvaluation of two autoinducer-2 quantification methods for application in marine environmentsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalJournal of Applied Microbiologyen
dc.eprint.versionPost-printen
dc.contributor.institutionCSIRO Land and Water; 147 Underwood Avenue Floreat WA 6014 Australiaen
kaust.authorWang, Tian-Nyuen
kaust.authorHong, Pei-Yingen
kaust.grant.numberURF/1/2982-01-01en
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