Engineering Plant Architecture via CRISPR/Cas9-mediated Alteration of Strigolactone Biosynthesis

Handle URI:
http://hdl.handle.net/10754/627057
Title:
Engineering Plant Architecture via CRISPR/Cas9-mediated Alteration of Strigolactone Biosynthesis
Authors:
Butt, Haroon; Jamil, Muhammad ( 0000-0003-0221-518X ) ; Wang, Jian You; Al-Babili, Salim ( 0000-0003-4823-2882 ) ; Mahfouz, Magdy M. ( 0000-0002-0616-6365 )
Abstract:
Precision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known for their role in inhibiting shoot branching (tillering). Here, we used CRISPR/Cas9 in rice (Oryza sativa) for targeted disruption of CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7), which controls a key step in SL biosynthesis. The ccd7 mutants exhibited a striking increase in tillering, combined with a dwarf phenotype, which could be rescued by application of the synthetic SL analog GR24. Striga germination assays and liquid chromatography mass spectrometry analysis showed that root exudates of ccd7 mutants were also SL deficient. Taken together, our results show the power of CRISPR/Cas9 for targeted engineering of plant architecture and for elucidating the molecular underpinnings of architecture-related traits.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Desert Agriculture Initiative; Laboratory for Genome Engineering
Citation:
Butt H, Jamil M, Wang JY, Al-Babili S, Mahfouz M (2018) Engineering Plant Architecture via CRISPR/Cas9-mediated Alteration of Strigolactone Biosynthesis. Available: http://dx.doi.org/10.1101/254698.
Publisher:
Cold Spring Harbor Laboratory
Issue Date:
28-Jan-2018
DOI:
10.1101/254698
Type:
Working Paper
Sponsors:
We would like to thank members of the laboratory for genome engineering at KAUST for their helpful discussions and critical reading of the manuscript. This study was supported by King Abdullah University of Science and Technology.
Additional Links:
https://www.biorxiv.org/content/early/2018/01/27/254698
Appears in Collections:
Other/General Submission; Bioscience Program; Center for Desert Agriculture; Plant Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorButt, Haroonen
dc.contributor.authorJamil, Muhammaden
dc.contributor.authorWang, Jian Youen
dc.contributor.authorAl-Babili, Salimen
dc.contributor.authorMahfouz, Magdy M.en
dc.date.accessioned2018-02-07T07:02:28Z-
dc.date.available2018-02-07T07:02:28Z-
dc.date.issued2018-01-28en
dc.identifier.citationButt H, Jamil M, Wang JY, Al-Babili S, Mahfouz M (2018) Engineering Plant Architecture via CRISPR/Cas9-mediated Alteration of Strigolactone Biosynthesis. Available: http://dx.doi.org/10.1101/254698.en
dc.identifier.doi10.1101/254698en
dc.identifier.urihttp://hdl.handle.net/10754/627057-
dc.description.abstractPrecision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known for their role in inhibiting shoot branching (tillering). Here, we used CRISPR/Cas9 in rice (Oryza sativa) for targeted disruption of CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7), which controls a key step in SL biosynthesis. The ccd7 mutants exhibited a striking increase in tillering, combined with a dwarf phenotype, which could be rescued by application of the synthetic SL analog GR24. Striga germination assays and liquid chromatography mass spectrometry analysis showed that root exudates of ccd7 mutants were also SL deficient. Taken together, our results show the power of CRISPR/Cas9 for targeted engineering of plant architecture and for elucidating the molecular underpinnings of architecture-related traits.en
dc.description.sponsorshipWe would like to thank members of the laboratory for genome engineering at KAUST for their helpful discussions and critical reading of the manuscript. This study was supported by King Abdullah University of Science and Technology.en
dc.publisherCold Spring Harbor Laboratoryen
dc.relation.urlhttps://www.biorxiv.org/content/early/2018/01/27/254698en
dc.rightsArchived with thanks to bioRxiven
dc.subjectgenome editingen
dc.subjectCRISPR/Cas9en
dc.subjectstrigolactonesen
dc.subjectplant architectureen
dc.subjectcarotenoidsen
dc.subjectcarotenoid cleavage dioxygenasesen
dc.subjectCCD7en
dc.subjectcrop improvementen
dc.subjectrice engineeringen
dc.titleEngineering Plant Architecture via CRISPR/Cas9-mediated Alteration of Strigolactone Biosynthesisen
dc.typeWorking Paperen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentPlant Science Programen
dc.contributor.departmentDesert Agriculture Initiativeen
dc.contributor.departmentLaboratory for Genome Engineeringen
dc.eprint.versionPre-printen
kaust.authorButt, Haroonen
kaust.authorJamil, Muhammaden
kaust.authorWang, Jian Youen
kaust.authorAl-Babili, Salimen
kaust.authorMahfouz, Magdy M.en
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