Reproductive gene expression in a coral reef fish exposed to increasing temperature across generations

Handle URI:
http://hdl.handle.net/10754/626808
Title:
Reproductive gene expression in a coral reef fish exposed to increasing temperature across generations
Authors:
Veilleux, Heather D; Donelson, Jennifer M; Munday, Philip L
Abstract:
Reproduction in marine fish is generally tightly linked with water temperature. Consequently, when adults are exposed to projected future ocean temperatures, reproductive output of many species declines precipitously. Recent research has shown that in the common reef fish, Acanthochromis polyacanthus, step-wise exposure to higher temperatures over two generations (parents: +1.5°C, offspring: +3.0°C) can improve reproductive output in the F2 generation compared to F2 fish that have experienced the same high temperatures over two generations (F1 parents: +3.0°C, F2 offspring: +3.0°C). To investigate how a step-wise increase in temperature between generations improved reproductive capacity, we tested the expression of well-known teleost reproductive genes in the brain and gonads of F2 fish using quantitative reverse transcription PCR and compared it among control (+0.0°C for two generations), developmental (+3.0°C in second generation only), step (+1.5°C in first generation and +3.0°C in second generation), and transgenerational (+3.0°C for two generations) treatments. We found that levels of gonadotropin receptor gene expression (Fshr and Lhcgr) in the testes were reduced in developmental and transgenerational temperature treatments, but were similar to control levels in the step treatment. This suggests Fshr and Lhcgr may be involved in regulating male reproductive capacity in A. polyacanthus. In addition, lower Fshb expression in the brain of females in all temperature treatments compared to control, suggests that Fshb expression, which is involved in vitellogenesis, is sensitive to high temperatures. Our results help elucidate key genes that facilitate successful reproduction in reef fishes when they experience a gradual increase in temperature across generations consistent with the trajectory of climate change.
Citation:
Veilleux HD, Donelson JM (2018) Reproductive gene expression in a coral reef fish exposed to increasing temperature across generations. Conservation Physiology 6. Available: http://dx.doi.org/10.1093/conphys/cox077.
Publisher:
Oxford University Press (OUP)
Journal:
Conservation Physiology
KAUST Grant Number:
OCRF-2014-CRG3-62140408
Issue Date:
7-Dec-2017
DOI:
10.1093/conphys/cox077
Type:
Article
ISSN:
2051-1434
Sponsors:
We would like to thank Professor Timothy Ravasi and the Integrative Systems Biology Lab at the King Abdullah University for Science and Technology (KAUST) for the A. polyacanthus genome used for primer design. In addition, thank you Professor Rhondda Jones for aiding in statistical interpretation and the members of the Molecular Ecology and Evolution Laboratory (MEEL), specifically Ms Alyssa Budd and Dr Roger Huerlimann. This study was supported by the ARC Centre of Excellence for Reef Studies and a Competitive Research Grant from the King Abdullah University of Science and Technology [OCRF-2014-CRG3-62140408].
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Full metadata record

DC FieldValue Language
dc.contributor.authorVeilleux, Heather Den
dc.contributor.authorDonelson, Jennifer Men
dc.contributor.authorMunday, Philip Len
dc.date.accessioned2018-01-15T06:49:45Z-
dc.date.available2018-01-15T06:49:45Z-
dc.date.issued2017-12-07en
dc.identifier.citationVeilleux HD, Donelson JM (2018) Reproductive gene expression in a coral reef fish exposed to increasing temperature across generations. Conservation Physiology 6. Available: http://dx.doi.org/10.1093/conphys/cox077.en
dc.identifier.issn2051-1434en
dc.identifier.doi10.1093/conphys/cox077en
dc.identifier.urihttp://hdl.handle.net/10754/626808-
dc.description.abstractReproduction in marine fish is generally tightly linked with water temperature. Consequently, when adults are exposed to projected future ocean temperatures, reproductive output of many species declines precipitously. Recent research has shown that in the common reef fish, Acanthochromis polyacanthus, step-wise exposure to higher temperatures over two generations (parents: +1.5°C, offspring: +3.0°C) can improve reproductive output in the F2 generation compared to F2 fish that have experienced the same high temperatures over two generations (F1 parents: +3.0°C, F2 offspring: +3.0°C). To investigate how a step-wise increase in temperature between generations improved reproductive capacity, we tested the expression of well-known teleost reproductive genes in the brain and gonads of F2 fish using quantitative reverse transcription PCR and compared it among control (+0.0°C for two generations), developmental (+3.0°C in second generation only), step (+1.5°C in first generation and +3.0°C in second generation), and transgenerational (+3.0°C for two generations) treatments. We found that levels of gonadotropin receptor gene expression (Fshr and Lhcgr) in the testes were reduced in developmental and transgenerational temperature treatments, but were similar to control levels in the step treatment. This suggests Fshr and Lhcgr may be involved in regulating male reproductive capacity in A. polyacanthus. In addition, lower Fshb expression in the brain of females in all temperature treatments compared to control, suggests that Fshb expression, which is involved in vitellogenesis, is sensitive to high temperatures. Our results help elucidate key genes that facilitate successful reproduction in reef fishes when they experience a gradual increase in temperature across generations consistent with the trajectory of climate change.en
dc.description.sponsorshipWe would like to thank Professor Timothy Ravasi and the Integrative Systems Biology Lab at the King Abdullah University for Science and Technology (KAUST) for the A. polyacanthus genome used for primer design. In addition, thank you Professor Rhondda Jones for aiding in statistical interpretation and the members of the Molecular Ecology and Evolution Laboratory (MEEL), specifically Ms Alyssa Budd and Dr Roger Huerlimann. This study was supported by the ARC Centre of Excellence for Reef Studies and a Competitive Research Grant from the King Abdullah University of Science and Technology [OCRF-2014-CRG3-62140408].en
dc.publisherOxford University Press (OUP)en
dc.titleReproductive gene expression in a coral reef fish exposed to increasing temperature across generationsen
dc.typeArticleen
dc.identifier.journalConservation Physiologyen
dc.contributor.institutionARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville QLD 4811, Australiaen
dc.contributor.institutionSchool of Life Sciences, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australiaen
kaust.grant.numberOCRF-2014-CRG3-62140408en
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