Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44

Handle URI:
http://hdl.handle.net/10754/626762
Title:
Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44
Authors:
Abu Samra, Dina Bashir Kamil ( 0000-0002-0974-5362 ) ; Aleisa, Fajr A ( 0000-0001-6403-374X ) ; Al-Amoodi, Asma ( 0000-0002-8970-3807 ) ; Jalal Ahmed, Heba M. ( 0000-0003-2172-2494 ) ; Chin, Chee Jia; AbuElela, Ayman ( 0000-0002-4529-3156 ) ; Bergam, Ptissam; Sougrat, Rachid ( 0000-0001-6476-1886 ) ; Merzaban, Jasmeen S. ( 0000-0002-7276-2907 )
Abstract:
CD34 is routinely used to identify and isolate human hematopoietic stem/progenitor cells (HSPCs) for use clinically in bone marrow transplantation, but its function on these cells remains elusive. Glycoprotein ligands on HSPCs help guide their migration to specialized microvascular beds in the bone marrow that express vascular selectins (E- and P-selectin). Here, we show that HSPC-enriched fractions from human hematopoietic tissue expressing CD34 (CD34pos) bound selectins, whereas those lacking CD34 (CD34neg) did not. An unbiased proteomics screen identified potential glycoprotein ligands on CD34pos cells revealing CD34 itself as a major vascular selectin ligand. Biochemical and CD34 knockdown analyses highlight a key role for CD34 in the first prerequisite step of cell migration, suggesting that it is not just a marker on these cells. Our results also entice future potential strategies to investigate the glycoforms of CD34 that discriminate normal HSPCs from leukemic cells and to manipulate CD34neg HSPC-enriched bone marrow or cord blood populations as a source of stem cells for clinical use.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Physical Sciences and Engineering (PSE) Division; Chemical and Biological Engineering Program; Bioscience Program; Imaging and Characterization Core Lab
Citation:
AbuSamra DB, Aleisa FA, Al-Amoodi AS, Jalal Ahmed HM, Chin CJ, et al. (2017) Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44. Blood Advances 1: 2799–2816. Available: http://dx.doi.org/10.1182/bloodadvances.2017004317.
Publisher:
American Society of Hematology
Journal:
Blood Advances
KAUST Grant Number:
CRG_R2_13_MERZ_KAUST_1
Issue Date:
27-Dec-2017
DOI:
10.1182/bloodadvances.2017004317
Type:
Article
ISSN:
2473-9529; 2473-9537
Sponsors:
The authors sincerely thank Samah Z. Gadhoum for her help and support throughout the project and Samir Hamdan for his time and fruitful discussions regarding the SPR analyses. The authors express their gratitude to Maryam Mih and Samar A. Rustum for their support in the management of the laboratory and also to Mohammad Imran Khan for his assistance with the confocal microscopy. Wei Xu at Imaging and Characterization core facility at King Abdullah University of Science and Technology (KAUST) was very helpful at providing analysis software and training on the confocal microscopes. The authors also thank Carolyn Unck from the KAUST Academic Writing Service for editing the manuscript. In addition, a special thanks to Aswini K. Panigrahi (MS assistance) and Alaguraj Dharmarajnadar (cell sorting on BD Influx) from the Bioscience Core Laboratory Facility at KAUST. This research was supported by a KAUST Faculty Baseline Research Funding Program and by a Competitive Research Grant (CRG_R2_13_MERZ_KAUST_1) (J.S.M.).
Additional Links:
http://www.bloodadvances.org/content/1/27/2799?sso-checked=true
Appears in Collections:
Articles; Bioscience Program; Advanced Nanofabrication, Imaging and Characterization Core Lab; Physical Sciences and Engineering (PSE) Division; Chemical and Biological Engineering Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorAbu Samra, Dina Bashir Kamilen
dc.contributor.authorAleisa, Fajr Aen
dc.contributor.authorAl-Amoodi, Asmaen
dc.contributor.authorJalal Ahmed, Heba M.en
dc.contributor.authorChin, Chee Jiaen
dc.contributor.authorAbuElela, Aymanen
dc.contributor.authorBergam, Ptissamen
dc.contributor.authorSougrat, Rachiden
dc.contributor.authorMerzaban, Jasmeen S.en
dc.date.accessioned2018-01-15T06:10:41Z-
dc.date.available2018-01-15T06:10:41Z-
dc.date.issued2017-12-27en
dc.identifier.citationAbuSamra DB, Aleisa FA, Al-Amoodi AS, Jalal Ahmed HM, Chin CJ, et al. (2017) Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44. Blood Advances 1: 2799–2816. Available: http://dx.doi.org/10.1182/bloodadvances.2017004317.en
dc.identifier.issn2473-9529en
dc.identifier.issn2473-9537en
dc.identifier.doi10.1182/bloodadvances.2017004317en
dc.identifier.urihttp://hdl.handle.net/10754/626762-
dc.description.abstractCD34 is routinely used to identify and isolate human hematopoietic stem/progenitor cells (HSPCs) for use clinically in bone marrow transplantation, but its function on these cells remains elusive. Glycoprotein ligands on HSPCs help guide their migration to specialized microvascular beds in the bone marrow that express vascular selectins (E- and P-selectin). Here, we show that HSPC-enriched fractions from human hematopoietic tissue expressing CD34 (CD34pos) bound selectins, whereas those lacking CD34 (CD34neg) did not. An unbiased proteomics screen identified potential glycoprotein ligands on CD34pos cells revealing CD34 itself as a major vascular selectin ligand. Biochemical and CD34 knockdown analyses highlight a key role for CD34 in the first prerequisite step of cell migration, suggesting that it is not just a marker on these cells. Our results also entice future potential strategies to investigate the glycoforms of CD34 that discriminate normal HSPCs from leukemic cells and to manipulate CD34neg HSPC-enriched bone marrow or cord blood populations as a source of stem cells for clinical use.en
dc.description.sponsorshipThe authors sincerely thank Samah Z. Gadhoum for her help and support throughout the project and Samir Hamdan for his time and fruitful discussions regarding the SPR analyses. The authors express their gratitude to Maryam Mih and Samar A. Rustum for their support in the management of the laboratory and also to Mohammad Imran Khan for his assistance with the confocal microscopy. Wei Xu at Imaging and Characterization core facility at King Abdullah University of Science and Technology (KAUST) was very helpful at providing analysis software and training on the confocal microscopes. The authors also thank Carolyn Unck from the KAUST Academic Writing Service for editing the manuscript. In addition, a special thanks to Aswini K. Panigrahi (MS assistance) and Alaguraj Dharmarajnadar (cell sorting on BD Influx) from the Bioscience Core Laboratory Facility at KAUST. This research was supported by a KAUST Faculty Baseline Research Funding Program and by a Competitive Research Grant (CRG_R2_13_MERZ_KAUST_1) (J.S.M.).en
dc.publisherAmerican Society of Hematologyen
dc.relation.urlhttp://www.bloodadvances.org/content/1/27/2799?sso-checked=trueen
dc.rightsArchived with thanks to Blood Advancesen
dc.titleNot just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44en
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentChemical and Biological Engineering Programen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentImaging and Characterization Core Laben
dc.identifier.journalBlood Advancesen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorAbu Samra, Dina Bashir Kamilen
kaust.authorAleisa, Fajr Aen
kaust.authorAl-Amoodi, Asmaen
kaust.authorJalal Ahmed, Heba M.en
kaust.authorChin, Chee Jiaen
kaust.authorAbuElela, Aymanen
kaust.authorBergam, Ptissamen
kaust.authorSougrat, Rachiden
kaust.authorMerzaban, Jasmeen S.en
kaust.grant.numberCRG_R2_13_MERZ_KAUST_1en
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