Label-free three-dimensional (3D) structural imaging in live cells using intrinsic optical refractive index

Handle URI:
http://hdl.handle.net/10754/626506
Title:
Label-free three-dimensional (3D) structural imaging in live cells using intrinsic optical refractive index
Authors:
Liu, Chen; Malek, Michael; Poon, Ivan K. H.; Jiang, Lanzhou; Sheppard, Colin J. R.; Roberts, Ann; Quiney, Harry; Zhang, Douguo; Yuan, Xiaocong; Lin, Jiao; Depeursinge, Christian; Marquet, Pierre; Kou, Shan Shan
Abstract:
Here we report a method for visualization of volumetric structural information of live biological samples with no exogenous contrast agents. The process is made possible through a technique that involves generation, synthesis and analysis of three-dimensional (3D) Fourier components of light diffracted by the sample. This leads to the direct recovery of quantitative cellular morphology with no iterative procedures for reduced computational complexity. Combing with the fact that the technique is easily adaptive to any imaging platform and requires minimum sample preparation, our proposed method is particularly promising for observing fast, volumetric and dynamic events previously only accessible through staining methods.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
arXiv
Issue Date:
14-Apr-2017
ARXIV:
arXiv:1704.04445
Type:
Preprint
Additional Links:
http://arxiv.org/abs/1704.04445v1; http://arxiv.org/pdf/1704.04445v1
Appears in Collections:
Other/General Submission; Other/General Submission; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLiu, Chenen
dc.contributor.authorMalek, Michaelen
dc.contributor.authorPoon, Ivan K. H.en
dc.contributor.authorJiang, Lanzhouen
dc.contributor.authorSheppard, Colin J. R.en
dc.contributor.authorRoberts, Annen
dc.contributor.authorQuiney, Harryen
dc.contributor.authorZhang, Douguoen
dc.contributor.authorYuan, Xiaocongen
dc.contributor.authorLin, Jiaoen
dc.contributor.authorDepeursinge, Christianen
dc.contributor.authorMarquet, Pierreen
dc.contributor.authorKou, Shan Shanen
dc.date.accessioned2017-12-28T07:32:13Z-
dc.date.available2017-12-28T07:32:13Z-
dc.date.issued2017-04-14en
dc.identifier.urihttp://hdl.handle.net/10754/626506-
dc.description.abstractHere we report a method for visualization of volumetric structural information of live biological samples with no exogenous contrast agents. The process is made possible through a technique that involves generation, synthesis and analysis of three-dimensional (3D) Fourier components of light diffracted by the sample. This leads to the direct recovery of quantitative cellular morphology with no iterative procedures for reduced computational complexity. Combing with the fact that the technique is easily adaptive to any imaging platform and requires minimum sample preparation, our proposed method is particularly promising for observing fast, volumetric and dynamic events previously only accessible through staining methods.en
dc.publisherarXiven
dc.relation.urlhttp://arxiv.org/abs/1704.04445v1en
dc.relation.urlhttp://arxiv.org/pdf/1704.04445v1en
dc.rightsArchived with thanks to arXiven
dc.titleLabel-free three-dimensional (3D) structural imaging in live cells using intrinsic optical refractive indexen
dc.typePreprinten
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.eprint.versionPre-printen
dc.contributor.institutionDepartment of Optics and Optical Engineering, Anhui Key Laboratory of Optoelectronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230026, China.en
dc.contributor.institutionDepartment of Chemistry and Physics, La Trobe Institute for Molecular Sciences (LIMS), La Trobe University, Melbourne, Victoria 3086, Australia.en
dc.contributor.institutionNanophotonics Research Centre, Shenzhen University & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.en
dc.contributor.institutionDepartment of Biochemistry and Genetics, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne, Victoria 3086, Australia.en
dc.contributor.institutionIstituto Italiano di Tecnologia, Genova 16163, Italy.en
dc.contributor.institutionSchool of Physics, University of Melbourne, VIC 3010 Australia.en
dc.contributor.institutionSchool of Engineering, RMIT University, Melbourne, VIC 3001, Australia.en
dc.contributor.institutionMicrovision and Microdiagnostic Group (SCI STI CHD), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.en
dc.contributor.institutionCentre d’optique, photonique et laser, Université Laval, Québec, Québec, Canada, Department of Psychiatry & Neuroscience, Université Laval, Québec, Québec, Canada.en
dc.contributor.institutionInstitut universitaire en santé mentale de Québec, Québec, Québec, Canada.en
dc.contributor.institutionJoint International Research Unit in Neurodevelopment and Child Psychiatry, CHUV, Département de Psychiatrie, Lausanne, Switzerland, Université Laval ,Québec, Québec, Canada.en
dc.identifier.arxividarXiv:1704.04445en
kaust.authorDepeursinge, Christianen
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