Spliceosomal protein U1A is involved in alternative splicing and salt stress tolerance in Arabidopsis thaliana

Handle URI:
http://hdl.handle.net/10754/626379
Title:
Spliceosomal protein U1A is involved in alternative splicing and salt stress tolerance in Arabidopsis thaliana
Authors:
Gu, Jinbao; Xia, Zhiqiang; Luo, Yuehua; Jiang, Xingyu; Qian, Bilian; Xie, He; Zhu, Jian-Kang; Xiong, Liming ( 0000-0001-8099-0806 ) ; Zhu, Jianhua; Wang, Zhen-Yu
Abstract:
Soil salinity is a significant threat to sustainable agricultural production worldwide. Plants must adjust their developmental and physiological processes to cope with salt stress. Although the capacity for adaptation ultimately depends on the genome, the exceptional versatility in gene regulation provided by the spliceosome-mediated alternative splicing (AS) is essential in these adaptive processes. However, the functions of the spliceosome in plant stress responses are poorly understood. Here, we report the in-depth characterization of a U1 spliceosomal protein, AtU1A, in controlling AS of pre-mRNAs under salt stress and salt stress tolerance in Arabidopsis thaliana. The atu1a mutant was hypersensitive to salt stress and accumulated more reactive oxygen species (ROS) than the wild-type under salt stress. RNA-seq analysis revealed that AtU1A regulates AS of many genes, presumably through modulating recognition of 5′ splice sites. We showed that AtU1A is associated with the pre-mRNA of the ROS detoxification-related gene ACO1 and is necessary for the regulation of ACO1 AS. ACO1 is important for salt tolerance because ectopic expression of ACO1 in the atu1a mutant can partially rescue its salt hypersensitive phenotype. Our findings highlight the critical role of AtU1A as a regulator of pre-mRNA processing and salt tolerance in plants.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Gu J, Xia Z, Luo Y, Jiang X, Qian B, et al. (2017) Spliceosomal protein U1A is involved in alternative splicing and salt stress tolerance in Arabidopsis thaliana. Nucleic Acids Research. Available: http://dx.doi.org/10.1093/nar/gkx1229.
Publisher:
Oxford University Press (OUP)
Journal:
Nucleic Acids Research
KAUST Grant Number:
BAS/1/1007-01-01
Issue Date:
1-Dec-2017
DOI:
10.1093/nar/gkx1229
Type:
Article
ISSN:
0305-1048; 1362-4962
Sponsors:
We acknowledge the ABRC for providing the T-DNA insertion lines. We thank Prof. Marvin Wickens (University of Wisconsin-Madison) for providing the yeast three-hybrid system. National Natural Science Foundation of China [31670250 to Z.W.]; Natural Science Foundation of Hainan Province [20163041 to Z.W.]; Hainan University Startup Fund [KYQD1562 to Z.W.]; YNTC [YNTC-2016YN22 to H.X.]; KAUST Faculty Baseline Funds [#BAS/1/1007-01-01 to L.M.X.]; National Key Technology Support Program [2015BAD01B02 to Y.H.L., X.Y.J.]; National Science Foundation [MCB0950242 to J.H.Z.]. Funding for open access charge: Hainan University Startup Fund [KYQD1562].
Additional Links:
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkx1229/4708260
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorGu, Jinbaoen
dc.contributor.authorXia, Zhiqiangen
dc.contributor.authorLuo, Yuehuaen
dc.contributor.authorJiang, Xingyuen
dc.contributor.authorQian, Bilianen
dc.contributor.authorXie, Heen
dc.contributor.authorZhu, Jian-Kangen
dc.contributor.authorXiong, Limingen
dc.contributor.authorZhu, Jianhuaen
dc.contributor.authorWang, Zhen-Yuen
dc.date.accessioned2017-12-14T12:34:04Z-
dc.date.available2017-12-14T12:34:04Z-
dc.date.issued2017-12-01en
dc.identifier.citationGu J, Xia Z, Luo Y, Jiang X, Qian B, et al. (2017) Spliceosomal protein U1A is involved in alternative splicing and salt stress tolerance in Arabidopsis thaliana. Nucleic Acids Research. Available: http://dx.doi.org/10.1093/nar/gkx1229.en
dc.identifier.issn0305-1048en
dc.identifier.issn1362-4962en
dc.identifier.doi10.1093/nar/gkx1229en
dc.identifier.urihttp://hdl.handle.net/10754/626379-
dc.description.abstractSoil salinity is a significant threat to sustainable agricultural production worldwide. Plants must adjust their developmental and physiological processes to cope with salt stress. Although the capacity for adaptation ultimately depends on the genome, the exceptional versatility in gene regulation provided by the spliceosome-mediated alternative splicing (AS) is essential in these adaptive processes. However, the functions of the spliceosome in plant stress responses are poorly understood. Here, we report the in-depth characterization of a U1 spliceosomal protein, AtU1A, in controlling AS of pre-mRNAs under salt stress and salt stress tolerance in Arabidopsis thaliana. The atu1a mutant was hypersensitive to salt stress and accumulated more reactive oxygen species (ROS) than the wild-type under salt stress. RNA-seq analysis revealed that AtU1A regulates AS of many genes, presumably through modulating recognition of 5′ splice sites. We showed that AtU1A is associated with the pre-mRNA of the ROS detoxification-related gene ACO1 and is necessary for the regulation of ACO1 AS. ACO1 is important for salt tolerance because ectopic expression of ACO1 in the atu1a mutant can partially rescue its salt hypersensitive phenotype. Our findings highlight the critical role of AtU1A as a regulator of pre-mRNA processing and salt tolerance in plants.en
dc.description.sponsorshipWe acknowledge the ABRC for providing the T-DNA insertion lines. We thank Prof. Marvin Wickens (University of Wisconsin-Madison) for providing the yeast three-hybrid system. National Natural Science Foundation of China [31670250 to Z.W.]; Natural Science Foundation of Hainan Province [20163041 to Z.W.]; Hainan University Startup Fund [KYQD1562 to Z.W.]; YNTC [YNTC-2016YN22 to H.X.]; KAUST Faculty Baseline Funds [#BAS/1/1007-01-01 to L.M.X.]; National Key Technology Support Program [2015BAD01B02 to Y.H.L., X.Y.J.]; National Science Foundation [MCB0950242 to J.H.Z.]. Funding for open access charge: Hainan University Startup Fund [KYQD1562].en
dc.publisherOxford University Press (OUP)en
dc.relation.urlhttps://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkx1229/4708260en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.titleSpliceosomal protein U1A is involved in alternative splicing and salt stress tolerance in Arabidopsis thalianaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalNucleic Acids Researchen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionHainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, Chinaen
dc.contributor.institutionInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, Chinaen
dc.contributor.institutionDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USAen
dc.contributor.institutionTobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming 650021, Chinaen
dc.contributor.institutionShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, Chinaen
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47906, USAen
kaust.authorXiong, Limingen
kaust.grant.numberBAS/1/1007-01-01en
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