A donor-specific QTL, exhibiting allelic variation for leaf sheath hairiness in a nested association mapping population, is located on barley chromosome 4H

Handle URI:
http://hdl.handle.net/10754/626365
Title:
A donor-specific QTL, exhibiting allelic variation for leaf sheath hairiness in a nested association mapping population, is located on barley chromosome 4H
Authors:
Saade, Stephanie; Kutlu, Burcu; Draba, Vera; Förster, Karin; Schumann, Erika; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Pillen, Klaus; Maurer, Andreas ( 0000-0002-2916-7475 )
Abstract:
Leaf sheath hairiness is a morphological trait associated with various advantages, including tolerance to both abiotic and biotic stresses, thereby increasing yield. Understanding the genetic basis of this trait in barley can therefore improve the agronomic performance of this economically important crop. We scored leaf sheath hairiness in a two-year field trial in 1,420 BC1S3 lines from the wild barley nested association mapping (NAM) population HEB-25. Leaf sheath hairiness segregated in six out of 25 families with the reference parent Barke being glabrous. We detected the major hairy leaf sheath locus Hs (syn. Hsh) on chromosome 4H (111.3 cM) with high precision. The effects of the locus varied across the six different wild barley donors, with donor of HEB family 11 conferring the highest score of leaf sheath hairiness. Due to the high mapping resolution present in HEB-25, we were able to discuss physically linked pentatricopeptide repeat genes and subtilisin-like proteases as potential candidate genes underlying this locus. In this study, we proved that HEB-25 provides an appropriate tool to further understand the genetic control of leaf sheath hairiness in barley. Furthermore, our work represents a perfect starting position to clone the gene responsible for the 4H locus observed.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Saade S, Kutlu B, Draba V, Förster K, Schumann E, et al. (2017) A donor-specific QTL, exhibiting allelic variation for leaf sheath hairiness in a nested association mapping population, is located on barley chromosome 4H. PLOS ONE 12: e0189446. Available: http://dx.doi.org/10.1371/journal.pone.0189446.
Publisher:
Public Library of Science (PLoS)
Journal:
PLOS ONE
Issue Date:
7-Dec-2017
DOI:
10.1371/journal.pone.0189446
Type:
Article
ISSN:
1932-6203
Sponsors:
This project was funded by German Research Foundation (DFG) via Grants Pi339/7-1 and Pi339/8-1 (AM): http://www.dfg.de/; Interdisciplinary Centre for Crop Plant Research (IZN), Halle (VD): http://www.uni-halle.de/izn/; King Abdullah University of Science and Technology (KAUST) (SS): https://www.kaust.edu.sa/en; Ege University Erasmus Programme (BK): http://ebys.ege.edu.tr/ogrenci/ebp/llp-erasmus.htm.
Additional Links:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189446
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorSaade, Stephanieen
dc.contributor.authorKutlu, Burcuen
dc.contributor.authorDraba, Veraen
dc.contributor.authorFörster, Karinen
dc.contributor.authorSchumann, Erikaen
dc.contributor.authorTester, Mark A.en
dc.contributor.authorPillen, Klausen
dc.contributor.authorMaurer, Andreasen
dc.date.accessioned2017-12-14T12:34:03Z-
dc.date.available2017-12-14T12:34:03Z-
dc.date.issued2017-12-07en
dc.identifier.citationSaade S, Kutlu B, Draba V, Förster K, Schumann E, et al. (2017) A donor-specific QTL, exhibiting allelic variation for leaf sheath hairiness in a nested association mapping population, is located on barley chromosome 4H. PLOS ONE 12: e0189446. Available: http://dx.doi.org/10.1371/journal.pone.0189446.en
dc.identifier.issn1932-6203en
dc.identifier.doi10.1371/journal.pone.0189446en
dc.identifier.urihttp://hdl.handle.net/10754/626365-
dc.description.abstractLeaf sheath hairiness is a morphological trait associated with various advantages, including tolerance to both abiotic and biotic stresses, thereby increasing yield. Understanding the genetic basis of this trait in barley can therefore improve the agronomic performance of this economically important crop. We scored leaf sheath hairiness in a two-year field trial in 1,420 BC1S3 lines from the wild barley nested association mapping (NAM) population HEB-25. Leaf sheath hairiness segregated in six out of 25 families with the reference parent Barke being glabrous. We detected the major hairy leaf sheath locus Hs (syn. Hsh) on chromosome 4H (111.3 cM) with high precision. The effects of the locus varied across the six different wild barley donors, with donor of HEB family 11 conferring the highest score of leaf sheath hairiness. Due to the high mapping resolution present in HEB-25, we were able to discuss physically linked pentatricopeptide repeat genes and subtilisin-like proteases as potential candidate genes underlying this locus. In this study, we proved that HEB-25 provides an appropriate tool to further understand the genetic control of leaf sheath hairiness in barley. Furthermore, our work represents a perfect starting position to clone the gene responsible for the 4H locus observed.en
dc.description.sponsorshipThis project was funded by German Research Foundation (DFG) via Grants Pi339/7-1 and Pi339/8-1 (AM): http://www.dfg.de/; Interdisciplinary Centre for Crop Plant Research (IZN), Halle (VD): http://www.uni-halle.de/izn/; King Abdullah University of Science and Technology (KAUST) (SS): https://www.kaust.edu.sa/en; Ege University Erasmus Programme (BK): http://ebys.ege.edu.tr/ogrenci/ebp/llp-erasmus.htm.en
dc.publisherPublic Library of Science (PLoS)en
dc.relation.urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189446en
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleA donor-specific QTL, exhibiting allelic variation for leaf sheath hairiness in a nested association mapping population, is located on barley chromosome 4Hen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPLOS ONEen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionEge University, Department of Biotechnology, Erzene, Bornova/İzmir, Turkey.en
dc.contributor.institutionInstitute of Agricultural and Nutritional Sciences, Department of Plant Breeding, Martin Luther University Halle-Wittenberg, Halle, Germany.en
dc.contributor.institutionInstitute of Agricultural and Nutritional Sciences, Department of Agronomy and Organic Farming, Martin Luther University Halle-Wittenberg, Halle, Germany.en
kaust.authorSaade, Stephanieen
kaust.authorTester, Mark A.en
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