Increasing tetracycline concentrations on the performance and communities of mixed microalgae-bacteria photo-bioreactors

Handle URI:
http://hdl.handle.net/10754/626358
Title:
Increasing tetracycline concentrations on the performance and communities of mixed microalgae-bacteria photo-bioreactors
Authors:
Xiong, Yanghui ( 0000-0002-5009-547X ) ; Hozic, Dzenan; Goncalves, Ana L.; Simões, Manuel; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
This study investigated the impact of varying concentrations of tetracycline on the performance of mixed microalgae-bacteria photo-bioreactors. Photo-bioreactors were assessed for their ability to remove carbon dioxide (CO2) from the biogas of anaerobic membrane bioreactor (anMBR), and nutrients from the anaerobic effluent. The varying concentrations of tetracycline had no impact on the removal of CO2 from biogas. 29% v/v of CO2 was completely removed to generate >20% v/v of oxygen (O2) in all reactors. Removal of nutrients and biomass was not affected at low concentrations of tetracycline (≤150μg/L), but 20mg/L of tetracycline lowered the biomass generation and removal efficiencies of phosphate. Conversely, high chlorophyll a and b content was observed at 20mg/L of tetracycline. High tetracycline level had no impact on the diversity of 18S rRNA gene-based microalgal communities but adversely affected the 16S rRNA gene-based microbial communities. Specifically, both Proteobacteria and Bacteroidetes phyla decreased in relative abundance but not phylum Chloroplast. Additionally, both nitrogen-fixing (e.g. Flavobacterium, unclassified Burkholderiales and unclassified Rhizobiaceae) and denitrifying groups (e.g. Hydrogenophaga spp.) were significantly reduced in relative abundance at high tetracycline concentration. Phosphate-accumulating microorganisms, Acinetobacter spp. and Pseudomonas spp. were similarly reduced upon exposure to high tetracycline concentration. Unclassified Comamonadaceae, however, increased in relative abundance, which correlated with an increase in the abundance of tetracycline resistance genes associated with efflux pump mechanism. Overall, the findings demonstrate that antibiotic concentrations in municipal wastewaters will not significantly affect the removal of nutrients by the mixed microalgae-bacteria photo-bioreactors. However, utilizing such photo-bioreactors as a polishing step for anMBRs that treat wastewaters with high tetracycline concentration may not be effective as evidenced from the lower nutrient removal and occurrence of antibiotic resistance genes.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Xiong Y, Hozic D, Goncalves AL, Simões M, Hong P-Y (2018) Increasing tetracycline concentrations on the performance and communities of mixed microalgae-bacteria photo-bioreactors. Algal Research 29: 249–256. Available: http://dx.doi.org/10.1016/j.algal.2017.11.033.
Publisher:
Elsevier BV
Journal:
Algal Research
KAUST Grant Number:
FCC/1/1971-15-01
Issue Date:
11-Dec-2017
DOI:
10.1016/j.algal.2017.11.033
Type:
Article
ISSN:
2211-9264
Sponsors:
This work is funded by King Abdullah University of Science and Technology (KAUST) Competitive Center Funding FCC/1/1971-15-01 awarded to Pei-Ying Hong. The authors would like to thank Dr Benjamin C.K. Wong for providing the python script.
Additional Links:
http://www.sciencedirect.com/science/article/pii/S2211926417310391
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorXiong, Yanghuien
dc.contributor.authorHozic, Dzenanen
dc.contributor.authorGoncalves, Ana L.en
dc.contributor.authorSimões, Manuelen
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2017-12-12T12:25:46Z-
dc.date.available2017-12-12T12:25:46Z-
dc.date.issued2017-12-11en
dc.identifier.citationXiong Y, Hozic D, Goncalves AL, Simões M, Hong P-Y (2018) Increasing tetracycline concentrations on the performance and communities of mixed microalgae-bacteria photo-bioreactors. Algal Research 29: 249–256. Available: http://dx.doi.org/10.1016/j.algal.2017.11.033.en
dc.identifier.issn2211-9264en
dc.identifier.doi10.1016/j.algal.2017.11.033en
dc.identifier.urihttp://hdl.handle.net/10754/626358-
dc.description.abstractThis study investigated the impact of varying concentrations of tetracycline on the performance of mixed microalgae-bacteria photo-bioreactors. Photo-bioreactors were assessed for their ability to remove carbon dioxide (CO2) from the biogas of anaerobic membrane bioreactor (anMBR), and nutrients from the anaerobic effluent. The varying concentrations of tetracycline had no impact on the removal of CO2 from biogas. 29% v/v of CO2 was completely removed to generate >20% v/v of oxygen (O2) in all reactors. Removal of nutrients and biomass was not affected at low concentrations of tetracycline (≤150μg/L), but 20mg/L of tetracycline lowered the biomass generation and removal efficiencies of phosphate. Conversely, high chlorophyll a and b content was observed at 20mg/L of tetracycline. High tetracycline level had no impact on the diversity of 18S rRNA gene-based microalgal communities but adversely affected the 16S rRNA gene-based microbial communities. Specifically, both Proteobacteria and Bacteroidetes phyla decreased in relative abundance but not phylum Chloroplast. Additionally, both nitrogen-fixing (e.g. Flavobacterium, unclassified Burkholderiales and unclassified Rhizobiaceae) and denitrifying groups (e.g. Hydrogenophaga spp.) were significantly reduced in relative abundance at high tetracycline concentration. Phosphate-accumulating microorganisms, Acinetobacter spp. and Pseudomonas spp. were similarly reduced upon exposure to high tetracycline concentration. Unclassified Comamonadaceae, however, increased in relative abundance, which correlated with an increase in the abundance of tetracycline resistance genes associated with efflux pump mechanism. Overall, the findings demonstrate that antibiotic concentrations in municipal wastewaters will not significantly affect the removal of nutrients by the mixed microalgae-bacteria photo-bioreactors. However, utilizing such photo-bioreactors as a polishing step for anMBRs that treat wastewaters with high tetracycline concentration may not be effective as evidenced from the lower nutrient removal and occurrence of antibiotic resistance genes.en
dc.description.sponsorshipThis work is funded by King Abdullah University of Science and Technology (KAUST) Competitive Center Funding FCC/1/1971-15-01 awarded to Pei-Ying Hong. The authors would like to thank Dr Benjamin C.K. Wong for providing the python script.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S2211926417310391en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Algal Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Algal Research, 11 December 2017. DOI: 10.1016/j.algal.2017.11.033. © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectAnaerobic membrane bioreactoren
dc.subjectCO2 removalen
dc.subjectNutrient removalen
dc.subject18S rRNAen
dc.subject16S rRNAen
dc.subjectTetracycline resistance genesen
dc.titleIncreasing tetracycline concentrations on the performance and communities of mixed microalgae-bacteria photo-bioreactorsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalAlgal Researchen
dc.eprint.versionPost-printen
dc.contributor.institutionLEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugalen
kaust.authorXiong, Yanghuien
kaust.authorHozic, Dzenanen
kaust.authorHong, Pei-Yingen
kaust.grant.numberFCC/1/1971-15-01en
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