Suspended DNA structural characterization by TEM diffraction

Handle URI:
http://hdl.handle.net/10754/626286
Title:
Suspended DNA structural characterization by TEM diffraction
Authors:
Marini, Monica ( 0000-0001-8182-5239 ) ; Allione, Marco ( 0000-0003-0757-9791 ) ; Lopatin, Sergei; Moretti, Manola; Giugni, Andrea; Torre, Bruno; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
In this work, micro-fabrication, super-hydrophobic properties and a physiologically compatible preparation step are combined and tailored to obtain background free biological samples to be investigated by Transmission Electron Microscopy (TEM) diffraction technique. The validation was performed evaluating a well-known parameter such as the DNA interbases value. The diffraction spacing measured is in good agreement with those obtained by HRTEM direct metrology and by traditional X-Ray diffraction. This approach addresses single molecule studies in a simplified and reproducible straightforward way with respect to more conventional and widely used techniques. In addition, it overcomes the need of long and elaborated samples preparations: the sample is in its physiological environment and the HRTEM data acquisition occurs without any background interference, coating, staining or additional manipulation. The congruence in the results reported in this paper makes the application of this approach extremely promising towards those molecules for which crystallization remains a hurdle, such as cell membrane proteins and fibrillar proteins.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Imaging and Characterization Core Lab
Citation:
Marini M, Allione M, Lopatin S, Moretti M, Giugni A, et al. (2017) Suspended DNA structural characterization by TEM diffraction. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2017.11.020.
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
1-Dec-2017
DOI:
10.1016/j.mee.2017.11.020
Type:
Article
ISSN:
0167-9317
Sponsors:
The authors acknowledge financial support from the KAUST start-up funding and from Italian Ministry of Health under the projects: Project no.: GR-2010-2320665 and Project no.: GR-2010-2311677.
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0167931717303969
Appears in Collections:
Articles; Advanced Nanofabrication, Imaging and Characterization Core Lab; Physical Sciences and Engineering (PSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMarini, Monicaen
dc.contributor.authorAllione, Marcoen
dc.contributor.authorLopatin, Sergeien
dc.contributor.authorMoretti, Manolaen
dc.contributor.authorGiugni, Andreaen
dc.contributor.authorTorre, Brunoen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2017-12-05T06:12:00Z-
dc.date.available2017-12-05T06:12:00Z-
dc.date.issued2017-12-01en
dc.identifier.citationMarini M, Allione M, Lopatin S, Moretti M, Giugni A, et al. (2017) Suspended DNA structural characterization by TEM diffraction. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2017.11.020.en
dc.identifier.issn0167-9317en
dc.identifier.doi10.1016/j.mee.2017.11.020en
dc.identifier.urihttp://hdl.handle.net/10754/626286-
dc.description.abstractIn this work, micro-fabrication, super-hydrophobic properties and a physiologically compatible preparation step are combined and tailored to obtain background free biological samples to be investigated by Transmission Electron Microscopy (TEM) diffraction technique. The validation was performed evaluating a well-known parameter such as the DNA interbases value. The diffraction spacing measured is in good agreement with those obtained by HRTEM direct metrology and by traditional X-Ray diffraction. This approach addresses single molecule studies in a simplified and reproducible straightforward way with respect to more conventional and widely used techniques. In addition, it overcomes the need of long and elaborated samples preparations: the sample is in its physiological environment and the HRTEM data acquisition occurs without any background interference, coating, staining or additional manipulation. The congruence in the results reported in this paper makes the application of this approach extremely promising towards those molecules for which crystallization remains a hurdle, such as cell membrane proteins and fibrillar proteins.en
dc.description.sponsorshipThe authors acknowledge financial support from the KAUST start-up funding and from Italian Ministry of Health under the projects: Project no.: GR-2010-2320665 and Project no.: GR-2010-2311677.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0167931717303969en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microelectronic Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microelectronic Engineering, [, , (2017-12-01)] DOI: 10.1016/j.mee.2017.11.020 . © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectDNAen
dc.subjectSuper-hydrophobic devicesen
dc.subjectDiffractionen
dc.subjectInterbases distanceen
dc.titleSuspended DNA structural characterization by TEM diffractionen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentImaging and Characterization Core Laben
dc.identifier.journalMicroelectronic Engineeringen
dc.eprint.versionPost-printen
kaust.authorMarini, Monicaen
kaust.authorAllione, Marcoen
kaust.authorLopatin, Sergeien
kaust.authorMoretti, Manolaen
kaust.authorGiugni, Andreaen
kaust.authorTorre, Brunoen
kaust.authorDi Fabrizio, Enzo M.en
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