Nanostructured Membranes Functionalized with Gold Nanoparticles for Separation and Recovery of Monoclonal Antibodies

Handle URI:
http://hdl.handle.net/10754/626206
Title:
Nanostructured Membranes Functionalized with Gold Nanoparticles for Separation and Recovery of Monoclonal Antibodies
Authors:
Soldan, Giada ( 0000-0002-1716-6803 )
Abstract:
The need of purified biomolecules, such as proteins or antibodies, has required the biopharmaceutical industries to look for new recovering solutions to reduce time and costs of bioseparations. In the last decade, the emergent field of membrane chromatography has gained attention as possible substituent of the common used protein A affinity chromatography for bioseparations. In this scenario, gold nanoparticles can be used as means for offering affinity, mainly because of their biocompatible and reversible binding behavior, together with their high surface area-to-volume ratio, which offers a large number of binding sites. This work introduces a new procedure for purification of monoclonal antibodies based on polymeric membranes functionalized with gold nanoparticles. This novel approach shortens the process of purification by promoting selective binding of antibodies, while separating a mixture of biomolecules during a filtration process. The effects of gold nanoparticles and the surrounding ligand on the proteins adsorption and filtration are investigated. The results confirm that the functionalization helps in inducing a selective binding, preventing the non-selective one, and it also improves the selectivity of the separation process.
Advisors:
Nunes, Suzana Pereira ( 0000-0002-3669-138X )
Committee Member:
Sarathy, S. Mani ( 0000-0002-3975-6206 ) ; Fabrizio, Enzo Di ( 0000-0001-5886-4678 )
KAUST Department:
Physical Sciences and Engineering (PSE) Division
Program:
Chemical and Biological Engineering
Issue Date:
Nov-2017
Type:
Thesis
Appears in Collections:
Theses

Full metadata record

DC FieldValue Language
dc.contributor.advisorNunes, Suzana Pereiraen
dc.contributor.authorSoldan, Giadaen
dc.date.accessioned2017-11-26T08:43:12Z-
dc.date.available2017-11-26T08:43:12Z-
dc.date.issued2017-11-
dc.identifier.urihttp://hdl.handle.net/10754/626206-
dc.description.abstractThe need of purified biomolecules, such as proteins or antibodies, has required the biopharmaceutical industries to look for new recovering solutions to reduce time and costs of bioseparations. In the last decade, the emergent field of membrane chromatography has gained attention as possible substituent of the common used protein A affinity chromatography for bioseparations. In this scenario, gold nanoparticles can be used as means for offering affinity, mainly because of their biocompatible and reversible binding behavior, together with their high surface area-to-volume ratio, which offers a large number of binding sites. This work introduces a new procedure for purification of monoclonal antibodies based on polymeric membranes functionalized with gold nanoparticles. This novel approach shortens the process of purification by promoting selective binding of antibodies, while separating a mixture of biomolecules during a filtration process. The effects of gold nanoparticles and the surrounding ligand on the proteins adsorption and filtration are investigated. The results confirm that the functionalization helps in inducing a selective binding, preventing the non-selective one, and it also improves the selectivity of the separation process.en
dc.language.isoenen
dc.subjectprotein purificationen
dc.subjectMembrane Chromatographyen
dc.subjectdownstream processingen
dc.subjectbiosparationsen
dc.subjectmonoclonal antibodiesen
dc.subjectgold nanoparticlesen
dc.titleNanostructured Membranes Functionalized with Gold Nanoparticles for Separation and Recovery of Monoclonal Antibodiesen
dc.typeThesisen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
thesis.degree.grantorKing Abdullah University of Science and Technologyen
dc.contributor.committeememberSarathy, S. Manien
dc.contributor.committeememberFabrizio, Enzo Dien
thesis.degree.disciplineChemical and Biological Engineeringen
thesis.degree.nameMaster of Scienceen
dc.person.id137695en
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