Genetics of Na+ exclusion and salinity tolerance in Afghani durum wheat landraces

Handle URI:
http://hdl.handle.net/10754/626200
Title:
Genetics of Na+ exclusion and salinity tolerance in Afghani durum wheat landraces
Authors:
Shamaya, Nawar Jalal; Shavrukov, Yuri; Langridge, Peter; Roy, Stuart John ( 0000-0003-0411-9431 ) ; Tester, Mark A. ( 0000-0002-5085-8801 )
Abstract:
BackgroundSelecting for low concentration of Na+ in the shoot provides one approach for tackling salinity stress that adversely affects crop production. Novel alleles for Na+ exclusion can be identified and then introduced into elite crop cultivars.ResultsWe have identified loci associated with lower Na+ concentration in leaves of durum wheat landraces originating from Afghanistan. Seedlings of two F2 populations derived from crossings between Australian durum wheat (Jandaroi) and two Afghani landraces (AUS-14740 and AUS-14752) were grown hydroponically and evaluated for Na+ and K+ concentration in the third leaf. High heritability was found for both third leaf Na+ concentration and the K+/Na+ ratio in both populations. Further work focussed on line AUS-14740. Bulk segregant analysis using 9 K SNP markers identified two loci significantly associated with third leaf Na+ concentration. Marker regression analysis showed a strong association between all traits studied and a favourable allele originating from AUS-14740 located on the long arm of chromosome 4B.ConclusionsThe candidate gene in the relevant region of chromosome 4B is likely to be the high affinity K+ transporter B1 (HKT1;5-B1). A second locus associated with third leaf Na+ concentration was located on chromosome 3BL, with the favourable allele originating from Jandaroi; however, no candidate gene can be identified.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture
Citation:
Shamaya NJ, Shavrukov Y, Langridge P, Roy SJ, Tester M (2017) Genetics of Na+ exclusion and salinity tolerance in Afghani durum wheat landraces. BMC Plant Biology 17. Available: http://dx.doi.org/10.1186/s12870-017-1164-6.
Publisher:
Springer Nature
Journal:
BMC Plant Biology
Issue Date:
21-Nov-2017
DOI:
10.1186/s12870-017-1164-6
Type:
Article
ISSN:
1471-2229
Sponsors:
This study was primarily supported by the Australian Centre for Plant Functional Genomics within The University of Adelaide through grants from the Australian Research Council and the Grains Research and Development Corporation. Financial support from King Abdullah University of Science and Technology (KAUST) is also gratefully acknowledged. The funders have no role in the study design, data analysis and interpretation, and manuscript writing.
Additional Links:
http://link.springer.com/article/10.1186/s12870-017-1164-6
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorShamaya, Nawar Jalalen
dc.contributor.authorShavrukov, Yurien
dc.contributor.authorLangridge, Peteren
dc.contributor.authorRoy, Stuart Johnen
dc.contributor.authorTester, Mark A.en
dc.date.accessioned2017-11-23T11:51:29Z-
dc.date.available2017-11-23T11:51:29Z-
dc.date.issued2017-11-21en
dc.identifier.citationShamaya NJ, Shavrukov Y, Langridge P, Roy SJ, Tester M (2017) Genetics of Na+ exclusion and salinity tolerance in Afghani durum wheat landraces. BMC Plant Biology 17. Available: http://dx.doi.org/10.1186/s12870-017-1164-6.en
dc.identifier.issn1471-2229en
dc.identifier.doi10.1186/s12870-017-1164-6en
dc.identifier.urihttp://hdl.handle.net/10754/626200-
dc.description.abstractBackgroundSelecting for low concentration of Na+ in the shoot provides one approach for tackling salinity stress that adversely affects crop production. Novel alleles for Na+ exclusion can be identified and then introduced into elite crop cultivars.ResultsWe have identified loci associated with lower Na+ concentration in leaves of durum wheat landraces originating from Afghanistan. Seedlings of two F2 populations derived from crossings between Australian durum wheat (Jandaroi) and two Afghani landraces (AUS-14740 and AUS-14752) were grown hydroponically and evaluated for Na+ and K+ concentration in the third leaf. High heritability was found for both third leaf Na+ concentration and the K+/Na+ ratio in both populations. Further work focussed on line AUS-14740. Bulk segregant analysis using 9 K SNP markers identified two loci significantly associated with third leaf Na+ concentration. Marker regression analysis showed a strong association between all traits studied and a favourable allele originating from AUS-14740 located on the long arm of chromosome 4B.ConclusionsThe candidate gene in the relevant region of chromosome 4B is likely to be the high affinity K+ transporter B1 (HKT1;5-B1). A second locus associated with third leaf Na+ concentration was located on chromosome 3BL, with the favourable allele originating from Jandaroi; however, no candidate gene can be identified.en
dc.description.sponsorshipThis study was primarily supported by the Australian Centre for Plant Functional Genomics within The University of Adelaide through grants from the Australian Research Council and the Grains Research and Development Corporation. Financial support from King Abdullah University of Science and Technology (KAUST) is also gratefully acknowledged. The funders have no role in the study design, data analysis and interpretation, and manuscript writing.en
dc.publisherSpringer Natureen
dc.relation.urlhttp://link.springer.com/article/10.1186/s12870-017-1164-6en
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectSalinity toleranceen
dc.subjectLeaf Na+ concentrationen
dc.subjectK+/Na+ ratioen
dc.subjectBulk Segregant analysisen
dc.subjectSNP markersen
dc.subjectHKT1;5en
dc.titleGenetics of Na+ exclusion and salinity tolerance in Afghani durum wheat landracesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalBMC Plant Biologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics, The University of Adelaide, Urrbrae, SA 5064, Australia.en
dc.contributor.institutionSchool of Agriculture, Food and Wine, The University of Adelaide, Urrbrae, SA 5064, Australia.en
dc.contributor.institutionDepartment of Field Crops, College of Agriculture, University of Baghdad, Al-Jadriyah, Baghdad, Iraq.en
kaust.authorTester, Mark A.en
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