Genetic Components of Root Architecture Remodeling in Response to Salt Stress

Handle URI:
http://hdl.handle.net/10754/626155
Title:
Genetic Components of Root Architecture Remodeling in Response to Salt Stress
Authors:
Julkowska, Magdalena; Koevoets, Iko Tamar; Mol, Selena; Hoefsloot, Huub CJ; Feron, Richard; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Keurentjes, Joost J.B.; Korte, Arthur; Haring, Michel A; de Boer, Gert-Jan; Testerink, Christa
Abstract:
Salinity of the soil is highly detrimental to plant growth. Plants respond by a redistribution of root mass between main and lateral roots, yet the genetic machinery underlying this process is still largely unknown. Here, we describe the natural variation among 347 Arabidopsis thaliana accessions in root system architecture (RSA) and identify the traits with highest natural variation in their response to salt. Salt-induced changes in RSA were associated with 100 genetic loci using genome-wide association studies (GWAS). Two candidate loci associated with lateral root development were validated and further investigated. Changes in CYP79B2 expression in salt stress positively correlated with lateral root development in accessions, and cyp79b2 cyp79b3 double mutants developed fewer and shorter lateral roots under salt stress, but not in control conditions. By contrast, high HKT1 expression in the root repressed lateral root development, which could be partially rescued by addition of potassium. The collected data and Multi-Variate analysis of multiple RSA traits, available through the Salt_NV_Root App, capture root responses to salinity. Together, our results provide a better understanding of effective RSA remodeling responses, and the genetic components involved, for plant performance in stress conditions.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Julkowska M, Koevoets IT, Mol S, Hoefsloot HC, Feron R, et al. (2017) Genetic Components of Root Architecture Remodeling in Response to Salt Stress. The Plant Cell: tpc.00680.2016. Available: http://dx.doi.org/10.1105/tpc.16.00680.
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
The Plant Cell
Issue Date:
7-Nov-2017
DOI:
10.1105/tpc.16.00680
Type:
Article
ISSN:
1040-4651; 1532-298X
Sponsors:
The authors would like to thank Willem Kruijer from Wageningen University for help with GWAS, Dorota Kawa and Jessica Meyer from University of Amsterdam for their technical support. We thank Dr. Hiroyushi Kasahara (RIKEN Center for Sustainable Resource Science, Yokohama, Japan) for the provided materials. This work was supported by the Netherlands Organisation for Scientific Research (NWO), STW Learning from Nature project 10987 and ALW Graduate Program grant 831.15.004.
Additional Links:
http://www.plantcell.org/content/early/2017/11/07/tpc.16.00680
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorJulkowska, Magdalenaen
dc.contributor.authorKoevoets, Iko Tamaren
dc.contributor.authorMol, Selenaen
dc.contributor.authorHoefsloot, Huub CJen
dc.contributor.authorFeron, Richarden
dc.contributor.authorTester, Mark A.en
dc.contributor.authorKeurentjes, Joost J.B.en
dc.contributor.authorKorte, Arthuren
dc.contributor.authorHaring, Michel Aen
dc.contributor.authorde Boer, Gert-Janen
dc.contributor.authorTesterink, Christaen
dc.date.accessioned2017-11-14T12:46:06Z-
dc.date.available2017-11-14T12:46:06Z-
dc.date.issued2017-11-07en
dc.identifier.citationJulkowska M, Koevoets IT, Mol S, Hoefsloot HC, Feron R, et al. (2017) Genetic Components of Root Architecture Remodeling in Response to Salt Stress. The Plant Cell: tpc.00680.2016. Available: http://dx.doi.org/10.1105/tpc.16.00680.en
dc.identifier.issn1040-4651en
dc.identifier.issn1532-298Xen
dc.identifier.doi10.1105/tpc.16.00680en
dc.identifier.urihttp://hdl.handle.net/10754/626155-
dc.description.abstractSalinity of the soil is highly detrimental to plant growth. Plants respond by a redistribution of root mass between main and lateral roots, yet the genetic machinery underlying this process is still largely unknown. Here, we describe the natural variation among 347 Arabidopsis thaliana accessions in root system architecture (RSA) and identify the traits with highest natural variation in their response to salt. Salt-induced changes in RSA were associated with 100 genetic loci using genome-wide association studies (GWAS). Two candidate loci associated with lateral root development were validated and further investigated. Changes in CYP79B2 expression in salt stress positively correlated with lateral root development in accessions, and cyp79b2 cyp79b3 double mutants developed fewer and shorter lateral roots under salt stress, but not in control conditions. By contrast, high HKT1 expression in the root repressed lateral root development, which could be partially rescued by addition of potassium. The collected data and Multi-Variate analysis of multiple RSA traits, available through the Salt_NV_Root App, capture root responses to salinity. Together, our results provide a better understanding of effective RSA remodeling responses, and the genetic components involved, for plant performance in stress conditions.en
dc.description.sponsorshipThe authors would like to thank Willem Kruijer from Wageningen University for help with GWAS, Dorota Kawa and Jessica Meyer from University of Amsterdam for their technical support. We thank Dr. Hiroyushi Kasahara (RIKEN Center for Sustainable Resource Science, Yokohama, Japan) for the provided materials. This work was supported by the Netherlands Organisation for Scientific Research (NWO), STW Learning from Nature project 10987 and ALW Graduate Program grant 831.15.004.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.plantcell.org/content/early/2017/11/07/tpc.16.00680en
dc.rightsArchived with thanks to The Plant Cellen
dc.titleGenetic Components of Root Architecture Remodeling in Response to Salt Stressen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalThe Plant Cellen
dc.eprint.versionPost-printen
dc.contributor.institutionUniversity of Amsterdam, Plant Cell Biology, Swammerdam Institute for Life Sciences, 1090GE Amsterdam, the Netherlandsen
dc.contributor.institutionUniversity of Amsterdam, Plant Physiology, Swammerdam Institute for Life Sciences, 1090GE Amsterdam, the Netherlandsen
dc.contributor.institutionUniversity of Amsterdam, Biosystems Data Analysis, Swammerdam Institute for Life Sciences, 1090GE Amsterdam, the Netherlandsen
dc.contributor.institutionENZA Zaden Research and Development B.V., 1602DB Enkhuizen, the Netherlandsen
dc.contributor.institutionLaboratory of Genetics, 6708PB Wageningen University & Research, Wageningen, the Netherlandsen
dc.contributor.institutionUniversity of Amsterdam, Applied Quantitative Genetics, Swammerdam Institute for Life Sciences, 1090GE Amsterdam, the Netherlandsen
dc.contributor.institutionCenter for Computational and Theoretical Biology, Wuerzburg Universitat, 97074 Wuerzburg, Germanyen
kaust.authorJulkowska, Magdalenaen
kaust.authorTester, Mark A.en
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