What is all this fuss about Tus? Comparison of recent findings from biophysical and biochemical experiments

Handle URI:
http://hdl.handle.net/10754/626136
Title:
What is all this fuss about Tus? Comparison of recent findings from biophysical and biochemical experiments
Authors:
Berghuis, Bojk A. ( 0000-0002-7797-0553 ) ; Raducanu, Vlad-Stefan ( 0000-0001-6722-9262 ) ; Elshenawy, Mohamed ( 0000-0002-8599-8388 ) ; Jergic, Slobodan; Depken, Martin; Dixon, Nicholas E. ( 0000-0002-5958-6945 ) ; Hamdan, Samir ( 0000-0001-5192-1852 ) ; Dekker, Nynke H. ( 0000-0003-4029-0973 )
Abstract:
Synchronizing the convergence of the two-oppositely moving DNA replication machineries at specific termination sites is a tightly coordinated process in bacteria. In Escherichia coli, a “replication fork trap” – found within a chromosomal region where forks are allowed to enter but not leave – is set by the protein–DNA roadblock Tus–Ter. The exact sequence of events by which Tus–Ter blocks replisomes approaching from one direction but not the other has been the subject of controversy for many decades. Specific protein–protein interactions between the nonpermissive face of Tus and the approaching helicase were challenged by biochemical and structural studies. These studies show that it is the helicase-induced strand separation that triggers the formation of new Tus–Ter interactions at the nonpermissive face – interactions that result in a highly stable “locked” complex. This controversy recently gained renewed attention as three single-molecule-based studies scrutinized this elusive Tus–Ter mechanism – leading to new findings and refinement of existing models, but also generating new questions. Here, we discuss and compare the findings of each of the single-molecule studies to find their common ground, pinpoint the crucial differences that remain, and push the understanding of this bipartite DNA–protein system further.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Berghuis BA, Raducanu V-S, Elshenawy MM, Jergic S, Depken M, et al. (2017) What is all this fuss about Tus? Comparison of recent findings from biophysical and biochemical experiments. Critical Reviews in Biochemistry and Molecular Biology: 1–15. Available: http://dx.doi.org/10.1080/10409238.2017.1394264.
Publisher:
Informa UK Limited
Journal:
Critical Reviews in Biochemistry and Molecular Biology
Issue Date:
7-Nov-2017
DOI:
10.1080/10409238.2017.1394264
Type:
Article
ISSN:
1040-9238; 1549-7798
Sponsors:
Funding for this work has been provided by the Australian Research Council (DP150100956) (to NED), by King Abdullah University of Science and Technology through core funding to (S.M.H.) and a Competitive Research Award (CRG5) (to S.M.H. and NED); and by a VICI grant from the Netherlands Organization for Scientific Research and an ERC Consolidator Grant (DynGenome, no 312221) from the European Research Council (both to N.H.D.).
Additional Links:
http://www.tandfonline.com/doi/full/10.1080/10409238.2017.1394264
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBerghuis, Bojk A.en
dc.contributor.authorRaducanu, Vlad-Stefanen
dc.contributor.authorElshenawy, Mohameden
dc.contributor.authorJergic, Slobodanen
dc.contributor.authorDepken, Martinen
dc.contributor.authorDixon, Nicholas E.en
dc.contributor.authorHamdan, Samiren
dc.contributor.authorDekker, Nynke H.en
dc.date.accessioned2017-11-09T06:33:07Z-
dc.date.available2017-11-09T06:33:07Z-
dc.date.issued2017-11-07en
dc.identifier.citationBerghuis BA, Raducanu V-S, Elshenawy MM, Jergic S, Depken M, et al. (2017) What is all this fuss about Tus? Comparison of recent findings from biophysical and biochemical experiments. Critical Reviews in Biochemistry and Molecular Biology: 1–15. Available: http://dx.doi.org/10.1080/10409238.2017.1394264.en
dc.identifier.issn1040-9238en
dc.identifier.issn1549-7798en
dc.identifier.doi10.1080/10409238.2017.1394264en
dc.identifier.urihttp://hdl.handle.net/10754/626136-
dc.description.abstractSynchronizing the convergence of the two-oppositely moving DNA replication machineries at specific termination sites is a tightly coordinated process in bacteria. In Escherichia coli, a “replication fork trap” – found within a chromosomal region where forks are allowed to enter but not leave – is set by the protein–DNA roadblock Tus–Ter. The exact sequence of events by which Tus–Ter blocks replisomes approaching from one direction but not the other has been the subject of controversy for many decades. Specific protein–protein interactions between the nonpermissive face of Tus and the approaching helicase were challenged by biochemical and structural studies. These studies show that it is the helicase-induced strand separation that triggers the formation of new Tus–Ter interactions at the nonpermissive face – interactions that result in a highly stable “locked” complex. This controversy recently gained renewed attention as three single-molecule-based studies scrutinized this elusive Tus–Ter mechanism – leading to new findings and refinement of existing models, but also generating new questions. Here, we discuss and compare the findings of each of the single-molecule studies to find their common ground, pinpoint the crucial differences that remain, and push the understanding of this bipartite DNA–protein system further.en
dc.description.sponsorshipFunding for this work has been provided by the Australian Research Council (DP150100956) (to NED), by King Abdullah University of Science and Technology through core funding to (S.M.H.) and a Competitive Research Award (CRG5) (to S.M.H. and NED); and by a VICI grant from the Netherlands Organization for Scientific Research and an ERC Consolidator Grant (DynGenome, no 312221) from the European Research Council (both to N.H.D.).en
dc.publisherInforma UK Limiteden
dc.relation.urlhttp://www.tandfonline.com/doi/full/10.1080/10409238.2017.1394264en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectDNA–protein interactionsen
dc.subjectDNA replicationen
dc.subjectprokaryotic replicationen
dc.subjectreplication terminationen
dc.subjectreplisomeen
dc.subjectTus–Teren
dc.subjectsingle-molecule techniquesen
dc.subjectmagnetic tweezersen
dc.titleWhat is all this fuss about Tus? Comparison of recent findings from biophysical and biochemical experimentsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalCritical Reviews in Biochemistry and Molecular Biologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Bionanoscience, Kavli institute of Nanoscience, Delft University of Technology, Delft, the Netherlands;en
dc.contributor.institutionCentre for Medical and Molecular Bioscience, University of Wollongong, Wollongong, New South Wales, Australiaen
kaust.authorRaducanu, Vlad-Stefanen
kaust.authorElshenawy, Mohameden
kaust.authorHamdan, Samiren
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