DNA methylation regulates transcriptional homeostasis of algal endosymbiosis in the coral model Aiptasia

Handle URI:
http://hdl.handle.net/10754/626113
Title:
DNA methylation regulates transcriptional homeostasis of algal endosymbiosis in the coral model Aiptasia
Authors:
Li, Yong ( 0000-0003-2609-2401 ) ; Liew, Yi Jin ( 0000-0003-2553-8870 ) ; Cui, Guoxin ( 0000-0003-4951-1883 ) ; Cziesielski, Maha J; Zahran, Noura Ibrahim Omar; Michell, Craig T; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Aranda, Manuel ( 0000-0001-6673-016X )
Abstract:
The symbiotic relationship between cnidarians and dinoflagellates is the cornerstone of coral reef ecosystems. Although research is focusing on the molecular mechanisms underlying this symbiosis, the role of epigenetic mechanisms, which have been implicated in transcriptional regulation and acclimation to environmental change, is unknown. To assess the role of DNA methylation in the cnidarian-dinoflagellate symbiosis, we analyzed genome-wide CpG methylation, histone associations, and transcriptomic states of symbiotic and aposymbiotic anemones in the model system Aiptasia. We find methylated genes are marked by histone H3K36me3 and show significant reduction of spurious transcription and transcriptional noise, revealing a role of DNA methylation in the maintenance of transcriptional homeostasis. Changes in DNA methylation and expression show enrichment for symbiosis-related processes such as immunity, apoptosis, phagocytosis recognition and phagosome formation, and unveil intricate interactions between the underlying pathways. Our results demonstrate that DNA methylation provides an epigenetic mechanism of transcriptional homeostasis during symbiosis.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Li Y, Liew YJ, Cui G, Cziesielski MJ, Zahran N, et al. (2017) DNA methylation regulates transcriptional homeostasis of algal endosymbiosis in the coral model Aiptasia . Available: http://dx.doi.org/10.1101/213066.
Publisher:
Cold Spring Harbor Laboratory
Issue Date:
3-Nov-2017
DOI:
10.1101/213066
Type:
Preprint
Sponsors:
Research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST). We thank Prof. John Pringle for the provision of the initial Aiptasia CC7 and Symbiodinium SSB01 cultures. We thank Sebastian Baumgarten for his help with establishing the Aiptasia cultures and critical reading of the manuscript.
Additional Links:
https://www.biorxiv.org/content/early/2017/11/02/213066
Appears in Collections:
Red Sea Research Center (RSRC); Other/General Submission; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Yongen
dc.contributor.authorLiew, Yi Jinen
dc.contributor.authorCui, Guoxinen
dc.contributor.authorCziesielski, Maha Jen
dc.contributor.authorZahran, Noura Ibrahim Omaren
dc.contributor.authorMichell, Craig Ten
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorAranda, Manuelen
dc.date.accessioned2017-11-06T07:09:04Z-
dc.date.available2017-11-06T07:09:04Z-
dc.date.issued2017-11-03en
dc.identifier.citationLi Y, Liew YJ, Cui G, Cziesielski MJ, Zahran N, et al. (2017) DNA methylation regulates transcriptional homeostasis of algal endosymbiosis in the coral model Aiptasia . Available: http://dx.doi.org/10.1101/213066.en
dc.identifier.doi10.1101/213066en
dc.identifier.urihttp://hdl.handle.net/10754/626113-
dc.description.abstractThe symbiotic relationship between cnidarians and dinoflagellates is the cornerstone of coral reef ecosystems. Although research is focusing on the molecular mechanisms underlying this symbiosis, the role of epigenetic mechanisms, which have been implicated in transcriptional regulation and acclimation to environmental change, is unknown. To assess the role of DNA methylation in the cnidarian-dinoflagellate symbiosis, we analyzed genome-wide CpG methylation, histone associations, and transcriptomic states of symbiotic and aposymbiotic anemones in the model system Aiptasia. We find methylated genes are marked by histone H3K36me3 and show significant reduction of spurious transcription and transcriptional noise, revealing a role of DNA methylation in the maintenance of transcriptional homeostasis. Changes in DNA methylation and expression show enrichment for symbiosis-related processes such as immunity, apoptosis, phagocytosis recognition and phagosome formation, and unveil intricate interactions between the underlying pathways. Our results demonstrate that DNA methylation provides an epigenetic mechanism of transcriptional homeostasis during symbiosis.en
dc.description.sponsorshipResearch reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST). We thank Prof. John Pringle for the provision of the initial Aiptasia CC7 and Symbiodinium SSB01 cultures. We thank Sebastian Baumgarten for his help with establishing the Aiptasia cultures and critical reading of the manuscript.en
dc.publisherCold Spring Harbor Laboratoryen
dc.relation.urlhttps://www.biorxiv.org/content/early/2017/11/02/213066en
dc.rightsThe copyright holder for this preprint is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.titleDNA methylation regulates transcriptional homeostasis of algal endosymbiosis in the coral model Aiptasiaen
dc.typePreprinten
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.eprint.versionPre-printen
kaust.authorLi, Yongen
kaust.authorLiew, Yi Jinen
kaust.authorCui, Guoxinen
kaust.authorCziesielski, Maha Jen
kaust.authorZahran, Noura Ibrahim Omaren
kaust.authorMichell, Craig Ten
kaust.authorVoolstra, Christian R.en
kaust.authorAranda, Manuelen
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