Direct protein quantification in complex sample solutions by surface-engineered nanorod probes

Handle URI:
http://hdl.handle.net/10754/626063
Title:
Direct protein quantification in complex sample solutions by surface-engineered nanorod probes
Authors:
Schrittwieser, Stefan ( 0000-0001-5807-3331 ) ; Pelaz, Beatriz; Parak, Wolfgang J. ( 0000-0003-1672-6650 ) ; Lentijo Mozo, Sergio; Soulantica, Katerina ( 0000-0003-3033-1620 ) ; Dieckhoff, Jan; Ludwig, Frank; Schotter, Joerg
Abstract:
Detecting biomarkers from complex sample solutions is the key objective of molecular diagnostics. Being able to do so in a simple approach that does not require laborious sample preparation, sophisticated equipment and trained staff is vital for point-of-care applications. Here, we report on the specific detection of the breast cancer biomarker sHER2 directly from serum and saliva samples by a nanorod-based homogeneous biosensing approach, which is easy to operate as it only requires mixing of the samples with the nanorod probes. By careful nanorod surface engineering and homogeneous assay design, we demonstrate that the formation of a protein corona around the nanoparticles does not limit the applicability of our detection method, but on the contrary enables us to conduct in-situ reference measurements, thus further strengthening the point-of-care applicability of our method. Making use of sandwich assays on top of the nanorods, we obtain a limit of detection of 110 pM and 470 pM in 10-fold diluted spiked saliva and serum samples, respectively. In conclusion, our results open up numerous applications in direct protein biomarker quantification, specifically in point-of-care settings where resources are limited and ease-of-use is of essence.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Schrittwieser S, Pelaz B, Parak WJ, Lentijo-Mozo S, Soulantica K, et al. (2017) Direct protein quantification in complex sample solutions by surface-engineered nanorod probes. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-04970-5.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
30-Jun-2017
DOI:
10.1038/s41598-017-04970-5
Type:
Article
ISSN:
2045-2322
Sponsors:
We thank Frauke Alves and Julia Bode from the Max-Planck-Institute of Experimental Medicine in Göttingen for the supply with the Herceptin therapeutic drug. We also want to thank David Fernandez and Amagoia Ametzazurra from Progenika Biopharma for providing the serum. This research was supported by the European Commission FP7 NAMDIATREAM project (EU NMP4-LA-2010–246479), and the German Research Foundation (DFG grant PA 794/25-1).
Additional Links:
https://www.nature.com/articles/s41598-017-04970-5
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorSchrittwieser, Stefanen
dc.contributor.authorPelaz, Beatrizen
dc.contributor.authorParak, Wolfgang J.en
dc.contributor.authorLentijo Mozo, Sergioen
dc.contributor.authorSoulantica, Katerinaen
dc.contributor.authorDieckhoff, Janen
dc.contributor.authorLudwig, Franken
dc.contributor.authorSchotter, Joergen
dc.date.accessioned2017-10-31T08:20:20Z-
dc.date.available2017-10-31T08:20:20Z-
dc.date.issued2017-06-30en
dc.identifier.citationSchrittwieser S, Pelaz B, Parak WJ, Lentijo-Mozo S, Soulantica K, et al. (2017) Direct protein quantification in complex sample solutions by surface-engineered nanorod probes. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-04970-5.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/s41598-017-04970-5en
dc.identifier.urihttp://hdl.handle.net/10754/626063-
dc.description.abstractDetecting biomarkers from complex sample solutions is the key objective of molecular diagnostics. Being able to do so in a simple approach that does not require laborious sample preparation, sophisticated equipment and trained staff is vital for point-of-care applications. Here, we report on the specific detection of the breast cancer biomarker sHER2 directly from serum and saliva samples by a nanorod-based homogeneous biosensing approach, which is easy to operate as it only requires mixing of the samples with the nanorod probes. By careful nanorod surface engineering and homogeneous assay design, we demonstrate that the formation of a protein corona around the nanoparticles does not limit the applicability of our detection method, but on the contrary enables us to conduct in-situ reference measurements, thus further strengthening the point-of-care applicability of our method. Making use of sandwich assays on top of the nanorods, we obtain a limit of detection of 110 pM and 470 pM in 10-fold diluted spiked saliva and serum samples, respectively. In conclusion, our results open up numerous applications in direct protein biomarker quantification, specifically in point-of-care settings where resources are limited and ease-of-use is of essence.en
dc.description.sponsorshipWe thank Frauke Alves and Julia Bode from the Max-Planck-Institute of Experimental Medicine in Göttingen for the supply with the Herceptin therapeutic drug. We also want to thank David Fernandez and Amagoia Ametzazurra from Progenika Biopharma for providing the serum. This research was supported by the European Commission FP7 NAMDIATREAM project (EU NMP4-LA-2010–246479), and the German Research Foundation (DFG grant PA 794/25-1).en
dc.publisherSpringer Natureen
dc.relation.urlhttps://www.nature.com/articles/s41598-017-04970-5en
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleDirect protein quantification in complex sample solutions by surface-engineered nanorod probesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionMolecular Diagnostics, AIT Austrian Institute of Technology, Vienna, , Austriaen
dc.contributor.institutionCentro Singular de Investigacion en Quimica Biologica y Materiales Moleculares (CiQUS), Departamento de Física de Partículas, Universidade de Santiago de Compostela, Santiago de Compostela, , Spainen
dc.contributor.institutionFachbereich Physik, Philipps-Universität Marburg, Marburg, , Germanyen
dc.contributor.institutionCIC Biomagune, San Sebastian, , Spainen
dc.contributor.institutionLaboratoire de Physique et Chimie des Nano-objets (LPCNO), Université de Toulouse, INSA, UPS, CNRS, Toulouse, , , , , Franceen
dc.contributor.institutionDiagnostic and Interventional Radiology Department and Clinic, University Medical Center Hamburg-Eppendorf, Hamburg, , Germanyen
dc.contributor.institutionInstitute of Electrical Measurement and Fundamental Electrical Engineering, TU Braunschweig, Braunschweig, , Germanyen
kaust.authorLentijo Mozo, Sergioen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.