Ruegeria profundi sp. nov. and Ruegeria marisrubri sp. nov., isolated from the brine–seawater interface at Erba Deep in the Red Sea

Handle URI:
http://hdl.handle.net/10754/626040
Title:
Ruegeria profundi sp. nov. and Ruegeria marisrubri sp. nov., isolated from the brine–seawater interface at Erba Deep in the Red Sea
Authors:
Zhang, Guishan; Haroon, Mohamed; Zhang, Ruifu; Dong, Xiaoyan; Wang, Dandan; Liu, Yunpeng; Xun, Weibing; Dong, Xiuzhu; Stingl, Ulrich ( 0000-0002-0684-2597 )
Abstract:
Two moderately halophilic marine bacterial strains of the family Rhodobacteraceae, designated ZGT108T and ZGT118T, were isolated from the brine-seawater interface at Erba Deep in the Red Sea (Saudi Arabia). Cells of both strains were aerobic, rod-shaped, non-motile, and Gram-stain-negative. The sequence similarity of the 16S rRNA genes of strains ZGT108T and ZGT118T was 94.9 %. The highest 16S rRNA gene sequence similarity of strain ZGT108T to its closest relative, Ruegeria conchae JCM 17315T, was 98.9 %, while the 16S rRNA gene of ZGT118T was most closely related to that of Ruegeria intermedia LMG 25539T (97.7 % similarity). The sizes of the draft genomes as presented here are 4 258 055 bp (strain ZGT108T) and 4 012 109 bp (strain ZGT118T), and the G+C contents of the draft genomes are 56.68 mol% (ZGT108T) and 62.94 mol% (ZGT108T). The combined physiological, biochemical, phylogenetic and genotypic data supported placement of both strains in the genus Ruegeria and indicated that the two strains are distinct from each other as well as from all other members in the genus Ruegeria. This was also confirmed by low DNA-DNA hybridization values (<43.6 %) and low ANI values (<91.8 %) between both strains and the most closely related Ruegeria species. Therefore, we propose two novel species in the genus Ruegeria to accommodate these novel isolates: Ruegeriaprofundi sp. nov. (type strain ZGT108T=JCM 19518T=ACCC 19861T) and Ruegeriamarisrubri sp. nov. (type strain ZGT118T=JCM 19519T= ACCC 19862T).
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Zhang G, Haroon MF, Zhang R, Dong X, Wang D, et al. (2017) Ruegeria profundi sp. nov. and Ruegeria marisrubri sp. nov., isolated from the brine–seawater interface at Erba Deep in the Red Sea. International Journal of Systematic and Evolutionary Microbiology. Available: http://dx.doi.org/10.1099/ijsem.0.002344.
Publisher:
Microbiology Society
Journal:
International Journal of Systematic and Evolutionary Microbiology
Issue Date:
12-Oct-2017
DOI:
10.1099/ijsem.0.002344
Type:
Article
ISSN:
1466-5026; 1466-5034
Sponsors:
This work was supported by baseline funding from King Abdullah University of Science and Technology (KAUST) and the Saudi Economicand Development Company Research Excellence Award to U.S. Part of this work was supported by the USDA National Institute of Food and Agriculture, Hatch project 1012953.
Additional Links:
http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002344
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Guishanen
dc.contributor.authorHaroon, Mohameden
dc.contributor.authorZhang, Ruifuen
dc.contributor.authorDong, Xiaoyanen
dc.contributor.authorWang, Dandanen
dc.contributor.authorLiu, Yunpengen
dc.contributor.authorXun, Weibingen
dc.contributor.authorDong, Xiuzhuen
dc.contributor.authorStingl, Ulrichen
dc.date.accessioned2017-10-30T08:39:52Z-
dc.date.available2017-10-30T08:39:52Z-
dc.date.issued2017-10-12en
dc.identifier.citationZhang G, Haroon MF, Zhang R, Dong X, Wang D, et al. (2017) Ruegeria profundi sp. nov. and Ruegeria marisrubri sp. nov., isolated from the brine–seawater interface at Erba Deep in the Red Sea. International Journal of Systematic and Evolutionary Microbiology. Available: http://dx.doi.org/10.1099/ijsem.0.002344.en
dc.identifier.issn1466-5026en
dc.identifier.issn1466-5034en
dc.identifier.doi10.1099/ijsem.0.002344en
dc.identifier.urihttp://hdl.handle.net/10754/626040-
dc.description.abstractTwo moderately halophilic marine bacterial strains of the family Rhodobacteraceae, designated ZGT108T and ZGT118T, were isolated from the brine-seawater interface at Erba Deep in the Red Sea (Saudi Arabia). Cells of both strains were aerobic, rod-shaped, non-motile, and Gram-stain-negative. The sequence similarity of the 16S rRNA genes of strains ZGT108T and ZGT118T was 94.9 %. The highest 16S rRNA gene sequence similarity of strain ZGT108T to its closest relative, Ruegeria conchae JCM 17315T, was 98.9 %, while the 16S rRNA gene of ZGT118T was most closely related to that of Ruegeria intermedia LMG 25539T (97.7 % similarity). The sizes of the draft genomes as presented here are 4 258 055 bp (strain ZGT108T) and 4 012 109 bp (strain ZGT118T), and the G+C contents of the draft genomes are 56.68 mol% (ZGT108T) and 62.94 mol% (ZGT108T). The combined physiological, biochemical, phylogenetic and genotypic data supported placement of both strains in the genus Ruegeria and indicated that the two strains are distinct from each other as well as from all other members in the genus Ruegeria. This was also confirmed by low DNA-DNA hybridization values (<43.6 %) and low ANI values (<91.8 %) between both strains and the most closely related Ruegeria species. Therefore, we propose two novel species in the genus Ruegeria to accommodate these novel isolates: Ruegeriaprofundi sp. nov. (type strain ZGT108T=JCM 19518T=ACCC 19861T) and Ruegeriamarisrubri sp. nov. (type strain ZGT118T=JCM 19519T= ACCC 19862T).en
dc.description.sponsorshipThis work was supported by baseline funding from King Abdullah University of Science and Technology (KAUST) and the Saudi Economicand Development Company Research Excellence Award to U.S. Part of this work was supported by the USDA National Institute of Food and Agriculture, Hatch project 1012953.en
dc.publisherMicrobiology Societyen
dc.relation.urlhttp://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002344en
dc.subjectRuegeria profundien
dc.subjectbrine poolsen
dc.subjectRhodobacteraceaeen
dc.subjectRuegeria marisrubrien
dc.titleRuegeria profundi sp. nov. and Ruegeria marisrubri sp. nov., isolated from the brine–seawater interface at Erba Deep in the Red Seaen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalInternational Journal of Systematic and Evolutionary Microbiologyen
dc.contributor.institution​Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR Chinaen
dc.contributor.institution​State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR Chinaen
dc.contributor.institutionUniversity of Florida, UF/IFAS, Department of Microbiology & Cell Science, Fort Lauderdale Research and Education Center, Davie, FL 33314, USAen
kaust.authorZhang, Guishanen
kaust.authorHaroon, Mohameden
kaust.authorStingl, Ulrichen
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