Removal of antibiotic-resistant bacteria and antibiotic resistance genes affected by varying degrees of fouling on anaerobic microfiltration membranes

Handle URI:
http://hdl.handle.net/10754/626022
Title:
Removal of antibiotic-resistant bacteria and antibiotic resistance genes affected by varying degrees of fouling on anaerobic microfiltration membranes
Authors:
Cheng, Hong ( 0000-0002-4943-9060 ) ; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
An anaerobic membrane bioreactor was retrofitted with polyvinylidene fluoride (PVDF) microfiltration membrane units, each of which was fouled to a different extent. The membranes with different degrees of fouling were evaluated for their efficiencies in removing three antibiotic-resistant bacteria (ARB), namely, blaNDM-1-positive Escherichia coli PI-7, blaCTX-M-15-positive Klebsiella pneumoniae L7, and blaOXA-48-positive E. coli UPEC-RIY-4, as well as their associated plasmid-borne antibiotic resistance genes (ARGs). The results showed that the log removal values (LRVs) of ARGs correlated positively with the extent of membrane fouling and ranged from 1.9 to 3.9. New membranes with a minimal foulant layer could remove more than 5 log units of ARB. However, as the membranes progressed to subcritical fouling, the LRVs of ARB decreased at increasing operating transmembrane pressures (TMPs). The LRV recovered back to 5 when the membrane was critically fouled, and the achieved LRV remained stable at different operating TMPs. Furthermore, characterization of the surface attributed the removal of both the ARB and ARGs to adsorption, which was facilitated by an increasing hydrophobicity and a decreasing surface ζ potential as the membranes fouled. Our results indicate that both the TMP and the foulant layer synergistically affected ARB removal, but the foulant layer was the main factor that contributed to ARG removal.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Cheng H, Hong P-Y (2017) Removal of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes Affected by Varying Degrees of Fouling on Anaerobic Microfiltration Membranes. Environmental Science & Technology. Available: http://dx.doi.org/10.1021/acs.est.7b03798.
Publisher:
American Chemical Society (ACS)
Journal:
Environmental Science & Technology
KAUST Grant Number:
FCC/1/1971-06-01
Issue Date:
28-Sep-2017
DOI:
10.1021/acs.est.7b03798
Type:
Article
ISSN:
0013-936X; 1520-5851
Sponsors:
The authors thank Mr. Qingtian Guan for providing technical assistance with plasmid assemblies. The authors thank Dr. Tiannyu Wang for creating the GFP-positive E. coli PI-7 strain. This study is supported by KAUST Center Competitive Funding, grant no. FCC/1/1971-06-01, awarded to P.-Y.H.
Additional Links:
http://pubs.acs.org/doi/abs/10.1021/acs.est.7b03798
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorCheng, Hongen
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2017-10-30T08:39:51Z-
dc.date.available2017-10-30T08:39:51Z-
dc.date.issued2017-09-28en
dc.identifier.citationCheng H, Hong P-Y (2017) Removal of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes Affected by Varying Degrees of Fouling on Anaerobic Microfiltration Membranes. Environmental Science & Technology. Available: http://dx.doi.org/10.1021/acs.est.7b03798.en
dc.identifier.issn0013-936Xen
dc.identifier.issn1520-5851en
dc.identifier.doi10.1021/acs.est.7b03798en
dc.identifier.urihttp://hdl.handle.net/10754/626022-
dc.description.abstractAn anaerobic membrane bioreactor was retrofitted with polyvinylidene fluoride (PVDF) microfiltration membrane units, each of which was fouled to a different extent. The membranes with different degrees of fouling were evaluated for their efficiencies in removing three antibiotic-resistant bacteria (ARB), namely, blaNDM-1-positive Escherichia coli PI-7, blaCTX-M-15-positive Klebsiella pneumoniae L7, and blaOXA-48-positive E. coli UPEC-RIY-4, as well as their associated plasmid-borne antibiotic resistance genes (ARGs). The results showed that the log removal values (LRVs) of ARGs correlated positively with the extent of membrane fouling and ranged from 1.9 to 3.9. New membranes with a minimal foulant layer could remove more than 5 log units of ARB. However, as the membranes progressed to subcritical fouling, the LRVs of ARB decreased at increasing operating transmembrane pressures (TMPs). The LRV recovered back to 5 when the membrane was critically fouled, and the achieved LRV remained stable at different operating TMPs. Furthermore, characterization of the surface attributed the removal of both the ARB and ARGs to adsorption, which was facilitated by an increasing hydrophobicity and a decreasing surface ζ potential as the membranes fouled. Our results indicate that both the TMP and the foulant layer synergistically affected ARB removal, but the foulant layer was the main factor that contributed to ARG removal.en
dc.description.sponsorshipThe authors thank Mr. Qingtian Guan for providing technical assistance with plasmid assemblies. The authors thank Dr. Tiannyu Wang for creating the GFP-positive E. coli PI-7 strain. This study is supported by KAUST Center Competitive Funding, grant no. FCC/1/1971-06-01, awarded to P.-Y.H.en
dc.publisherAmerican Chemical Society (ACS)en
dc.relation.urlhttp://pubs.acs.org/doi/abs/10.1021/acs.est.7b03798en
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Environmental Science & Technology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.est.7b03798.en
dc.titleRemoval of antibiotic-resistant bacteria and antibiotic resistance genes affected by varying degrees of fouling on anaerobic microfiltration membranesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalEnvironmental Science & Technologyen
dc.eprint.versionPost-printen
kaust.authorCheng, Hongen
kaust.authorHong, Pei-Yingen
kaust.grant.numberFCC/1/1971-06-01en
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