Complete Genome Sequence Analysis of Enterobacter sp. SA187, a Plant Multi-Stress Tolerance Promoting Endophytic Bacterium

Handle URI:
http://hdl.handle.net/10754/625961
Title:
Complete Genome Sequence Analysis of Enterobacter sp. SA187, a Plant Multi-Stress Tolerance Promoting Endophytic Bacterium
Authors:
Andres-Barrao, Cristina; Lafi, Feras Fawzi; Alam, Intikhab; Zélicourt, Axel de ( 0000-0003-0797-6308 ) ; Eida, Abdul Aziz; Bokhari, Ameerah; Alzubaidy, Hanin S. ( 0000-0002-3388-4039 ) ; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Hirt, Heribert ( 0000-0003-3119-9633 ) ; Saad, Maged ( 0000-0002-5655-8674 )
Abstract:
Enterobacter sp. SA187 is an endophytic bacterium that has been isolated from root nodules of the indigenous desert plant Indigofera argentea. SA187 could survive in the rhizosphere as well as in association with different plant species, and was able to provide abiotic stress tolerance to Arabidopsis thaliana. The genome sequence of SA187 was obtained by using Pacific BioScience (PacBio) single-molecule sequencing technology, with average coverage of 275X. The genome of SA187 consists of one single 4,429,597 bp chromosome, with an average 56% GC content and 4,347 predicted protein coding DNA sequences (CDS), 153 ncRNA, 7 rRNA, and 84 tRNA. Functional analysis of the SA187 genome revealed a large number of genes involved in uptake and exchange of nutrients, chemotaxis, mobilization and plant colonization. A high number of genes were also found to be involved in survival, defense against oxidative stress and production of antimicrobial compounds and toxins. Moreover, different metabolic pathways were identified that potentially contribute to plant growth promotion. The information encoded in the genome of SA187 reveals the characteristics of a dualistic lifestyle of a bacterium that can adapt to different environments and promote the growth of plants. This information provides a better understanding of the mechanisms involved in plant-microbe interaction and could be further exploited to develop SA187 as a biological agent to improve agricultural practices in marginal and arid lands.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computational Bioscience Research Center (CBRC)
Citation:
Andrés-Barrao C, Lafi FF, Alam I, de Zélicourt A, Eida AA, et al. (2017) Complete Genome Sequence Analysis of Enterobacter sp. SA187, a Plant Multi-Stress Tolerance Promoting Endophytic Bacterium. Frontiers in Microbiology 8. Available: http://dx.doi.org/10.3389/fmicb.2017.02023.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Microbiology
KAUST Grant Number:
BAS/1/1062-01-01; URF/1/1976-02; FCS/1/2448-01
Issue Date:
20-Oct-2017
DOI:
10.3389/fmicb.2017.02023
Type:
Article
ISSN:
1664-302X
Sponsors:
This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) to HH No. BAS/1/1062-01-01, and by the KAUST Office of Sponsored Research (OSR) under Awards No. URF/1/1976-02 and FCS/1/2448-01 to VB.
Additional Links:
https://www.frontiersin.org/articles/10.3389/fmicb.2017.02023/full
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorAndres-Barrao, Cristinaen
dc.contributor.authorLafi, Feras Fawzien
dc.contributor.authorAlam, Intikhaben
dc.contributor.authorZélicourt, Axel deen
dc.contributor.authorEida, Abdul Azizen
dc.contributor.authorBokhari, Ameerahen
dc.contributor.authorAlzubaidy, Hanin S.en
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorHirt, Heriberten
dc.contributor.authorSaad, Mageden
dc.date.accessioned2017-10-30T07:55:29Z-
dc.date.available2017-10-30T07:55:29Z-
dc.date.issued2017-10-20en
dc.identifier.citationAndrés-Barrao C, Lafi FF, Alam I, de Zélicourt A, Eida AA, et al. (2017) Complete Genome Sequence Analysis of Enterobacter sp. SA187, a Plant Multi-Stress Tolerance Promoting Endophytic Bacterium. Frontiers in Microbiology 8. Available: http://dx.doi.org/10.3389/fmicb.2017.02023.en
dc.identifier.issn1664-302Xen
dc.identifier.doi10.3389/fmicb.2017.02023en
dc.identifier.urihttp://hdl.handle.net/10754/625961-
dc.description.abstractEnterobacter sp. SA187 is an endophytic bacterium that has been isolated from root nodules of the indigenous desert plant Indigofera argentea. SA187 could survive in the rhizosphere as well as in association with different plant species, and was able to provide abiotic stress tolerance to Arabidopsis thaliana. The genome sequence of SA187 was obtained by using Pacific BioScience (PacBio) single-molecule sequencing technology, with average coverage of 275X. The genome of SA187 consists of one single 4,429,597 bp chromosome, with an average 56% GC content and 4,347 predicted protein coding DNA sequences (CDS), 153 ncRNA, 7 rRNA, and 84 tRNA. Functional analysis of the SA187 genome revealed a large number of genes involved in uptake and exchange of nutrients, chemotaxis, mobilization and plant colonization. A high number of genes were also found to be involved in survival, defense against oxidative stress and production of antimicrobial compounds and toxins. Moreover, different metabolic pathways were identified that potentially contribute to plant growth promotion. The information encoded in the genome of SA187 reveals the characteristics of a dualistic lifestyle of a bacterium that can adapt to different environments and promote the growth of plants. This information provides a better understanding of the mechanisms involved in plant-microbe interaction and could be further exploited to develop SA187 as a biological agent to improve agricultural practices in marginal and arid lands.en
dc.description.sponsorshipThis publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) to HH No. BAS/1/1062-01-01, and by the KAUST Office of Sponsored Research (OSR) under Awards No. URF/1/1976-02 and FCS/1/2448-01 to VB.en
dc.publisherFrontiers Media SAen
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2017.02023/fullen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectIndigofera argenteaen
dc.subjectPlant growth-promoting bacteria (PGPB)en
dc.subjectPlant-microbe interactionen
dc.subjectRoot endophytesen
dc.subjectSalt stressen
dc.titleComplete Genome Sequence Analysis of Enterobacter sp. SA187, a Plant Multi-Stress Tolerance Promoting Endophytic Bacteriumen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalFrontiers in Microbiologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Medical Laboratories, Faculty of Science, American University of Madaba, Madaba, , Jordanen
kaust.authorAndres-Barrao, Cristinaen
kaust.authorLafi, Feras Fawzien
kaust.authorAlam, Intikhaben
kaust.authorZélicourt, Axel deen
kaust.authorEida, Abdul Azizen
kaust.authorBokhari, Ameerahen
kaust.authorAlzubaidy, Hanin S.en
kaust.authorBajic, Vladimir B.en
kaust.authorHirt, Heriberten
kaust.authorSaad, Mageden
kaust.grant.numberBAS/1/1062-01-01en
kaust.grant.numberURF/1/1976-02en
kaust.grant.numberFCS/1/2448-01en
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