Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity

Handle URI:
http://hdl.handle.net/10754/625933
Title:
Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity
Authors:
Orlando, Marta; Ravasenga, Tiziana; Petrini, Enrica Maria; Falqui, Andrea ( 0000-0002-1476-7742 ) ; Marotta, Roberto; Barberis, Andrea
Abstract:
Both excitatory and inhibitory synaptic contacts display activity dependent dynamic changes in their efficacy that are globally termed synaptic plasticity. Although the molecular mechanisms underlying glutamatergic synaptic plasticity have been extensively investigated and described, those responsible for inhibitory synaptic plasticity are only beginning to be unveiled. In this framework, the ultrastructural changes of the inhibitory synapses during plasticity have been poorly investigated. Here we combined confocal fluorescence microscopy (CFM) with high resolution scanning electron microscopy (HRSEM) to characterize the fine structural rearrangements of post-synaptic GABAA Receptors (GABAARs) at the nanometric scale during the induction of inhibitory long-term potentiation (iLTP). Additional electron tomography (ET) experiments on immunolabelled hippocampal neurons allowed the visualization of synaptic contacts and confirmed the reorganization of post-synaptic GABAAR clusters in response to chemical iLTP inducing protocol. Altogether, these approaches revealed that, following the induction of inhibitory synaptic potentiation, GABAAR clusters increase in size and number at the post-synaptic membrane with no other major structural changes of the pre- and post-synaptic elements.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Orlando M, Ravasenga T, Petrini EM, Falqui A, Marotta R, et al. (2017) Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-14210-5.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
17-Oct-2017
DOI:
10.1038/s41598-017-14210-5
Type:
Article
ISSN:
2045-2322
Sponsors:
We thank R. Ruffilli for her support in performing CL-HRSEM and ET experiments. RM is also grateful to A. Genovese and A. Casu for their friendship. This work has been supported by Telethon-Italy (GGP16083 to A.B.) and Compagnia di San Paolo (Grant ROL-9687 to Marti Duocastella and A.B.).
Additional Links:
https://www.nature.com/articles/s41598-017-14210-5
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorOrlando, Martaen
dc.contributor.authorRavasenga, Tizianaen
dc.contributor.authorPetrini, Enrica Mariaen
dc.contributor.authorFalqui, Andreaen
dc.contributor.authorMarotta, Robertoen
dc.contributor.authorBarberis, Andreaen
dc.date.accessioned2017-10-24T10:45:41Z-
dc.date.available2017-10-24T10:45:41Z-
dc.date.issued2017-10-17en
dc.identifier.citationOrlando M, Ravasenga T, Petrini EM, Falqui A, Marotta R, et al. (2017) Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-14210-5.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/s41598-017-14210-5en
dc.identifier.urihttp://hdl.handle.net/10754/625933-
dc.description.abstractBoth excitatory and inhibitory synaptic contacts display activity dependent dynamic changes in their efficacy that are globally termed synaptic plasticity. Although the molecular mechanisms underlying glutamatergic synaptic plasticity have been extensively investigated and described, those responsible for inhibitory synaptic plasticity are only beginning to be unveiled. In this framework, the ultrastructural changes of the inhibitory synapses during plasticity have been poorly investigated. Here we combined confocal fluorescence microscopy (CFM) with high resolution scanning electron microscopy (HRSEM) to characterize the fine structural rearrangements of post-synaptic GABAA Receptors (GABAARs) at the nanometric scale during the induction of inhibitory long-term potentiation (iLTP). Additional electron tomography (ET) experiments on immunolabelled hippocampal neurons allowed the visualization of synaptic contacts and confirmed the reorganization of post-synaptic GABAAR clusters in response to chemical iLTP inducing protocol. Altogether, these approaches revealed that, following the induction of inhibitory synaptic potentiation, GABAAR clusters increase in size and number at the post-synaptic membrane with no other major structural changes of the pre- and post-synaptic elements.en
dc.description.sponsorshipWe thank R. Ruffilli for her support in performing CL-HRSEM and ET experiments. RM is also grateful to A. Genovese and A. Casu for their friendship. This work has been supported by Telethon-Italy (GGP16083 to A.B.) and Compagnia di San Paolo (Grant ROL-9687 to Marti Duocastella and A.B.).en
dc.publisherSpringer Natureen
dc.relation.urlhttps://www.nature.com/articles/s41598-017-14210-5en
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleCorrelating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticityen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionNeuroscience and Brain Technologies Department, Fondazione Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genoa, Italyen
dc.contributor.institutionElectron Microscopy Facility, Fondazione Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genoa, Italyen
kaust.authorFalqui, Andreaen
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