Immune-modulatory genomic properties differentiate gut microbiota of infants with and without eczema

Handle URI:
http://hdl.handle.net/10754/625916
Title:
Immune-modulatory genomic properties differentiate gut microbiota of infants with and without eczema
Authors:
Oh, Seungdae; Yap, Gaik Chin; Hong, Pei-Ying ( 0000-0002-4474-6600 ) ; Huang, Chiung-Hui; Aw, Marion M.; Shek, Lynette Pei-Chi; Liu, Wen-Tso; Lee, Bee Wah
Abstract:
Gut microbiota play an important role in human immunological processes, potentially affecting allergic diseases such as eczema. The diversity and structure of gut microbiota in infants with eczema have been previously documented. This study aims to evaluate by comparative metagenomics differences in genetic content in gut microbiota of infants with eczema and their matched controls. Stools were collected at the age of one month old from twelve infants from an at risk birth cohort in a case control manner. Clinical follow up for atopic outcomes were carried out at the age of 12 and 24 months. Microbial genomic DNA were extracted from stool samples and used for shotgun sequencing. Comparative metagenomic analysis showed that immune-regulatory TCAAGCTTGA motifs were significantly enriched in the six healthy controls (C) communities compared to the six eczema subjects (E), with many encoded by Bifidobacterium (38% of the total motifs in the C communities). Draft genomes of five Bifidobacterium species populations (B. longum, B. bifidum, B. breve, B. dentium, and B. pseudocatenulatum) were recovered from metagenomic datasets. The B. longum BFN-121-2 genome encoded more TCAAGCTTGA motifs (4.2 copies per one million genome sequence) than other Bifidobacterium genomes. Additionally, the communities in the stool of controls (C) were also significantly enriched in functions associated with tetrapyrrole biosynthesis compared to those of eczema (E). Our results show distinct immune-modulatory genomic properties of gut microbiota in infants associated with eczema and provide new insights into potential role of gut microbiota in affecting human immune homeostasis.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Oh S, Yap GC, Hong P-Y, Huang C-H, Aw MM, et al. (2017) Immune-modulatory genomic properties differentiate gut microbiota of infants with and without eczema. PLOS ONE 12: e0184955. Available: http://dx.doi.org/10.1371/journal.pone.0184955.
Publisher:
Public Library of Science (PLoS)
Journal:
PLOS ONE
Issue Date:
19-Oct-2017
DOI:
10.1371/journal.pone.0184955
Type:
Article
ISSN:
1932-6203
Sponsors:
This study was supported by MOH’s National Medical Research Council (NMRC/EDG/1057/2011 and NMRC/CIRG/1414/2014). Seungdae Oh was supported by the start-up grant from the University of Illinois at Urbana-Champaign (to WTL) and Nanyang Technological University (to SO), respectively.
Additional Links:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0184955
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorOh, Seungdaeen
dc.contributor.authorYap, Gaik Chinen
dc.contributor.authorHong, Pei-Yingen
dc.contributor.authorHuang, Chiung-Huien
dc.contributor.authorAw, Marion M.en
dc.contributor.authorShek, Lynette Pei-Chien
dc.contributor.authorLiu, Wen-Tsoen
dc.contributor.authorLee, Bee Wahen
dc.date.accessioned2017-10-22T11:48:13Z-
dc.date.available2017-10-22T11:48:13Z-
dc.date.issued2017-10-19en
dc.identifier.citationOh S, Yap GC, Hong P-Y, Huang C-H, Aw MM, et al. (2017) Immune-modulatory genomic properties differentiate gut microbiota of infants with and without eczema. PLOS ONE 12: e0184955. Available: http://dx.doi.org/10.1371/journal.pone.0184955.en
dc.identifier.issn1932-6203en
dc.identifier.doi10.1371/journal.pone.0184955en
dc.identifier.urihttp://hdl.handle.net/10754/625916-
dc.description.abstractGut microbiota play an important role in human immunological processes, potentially affecting allergic diseases such as eczema. The diversity and structure of gut microbiota in infants with eczema have been previously documented. This study aims to evaluate by comparative metagenomics differences in genetic content in gut microbiota of infants with eczema and their matched controls. Stools were collected at the age of one month old from twelve infants from an at risk birth cohort in a case control manner. Clinical follow up for atopic outcomes were carried out at the age of 12 and 24 months. Microbial genomic DNA were extracted from stool samples and used for shotgun sequencing. Comparative metagenomic analysis showed that immune-regulatory TCAAGCTTGA motifs were significantly enriched in the six healthy controls (C) communities compared to the six eczema subjects (E), with many encoded by Bifidobacterium (38% of the total motifs in the C communities). Draft genomes of five Bifidobacterium species populations (B. longum, B. bifidum, B. breve, B. dentium, and B. pseudocatenulatum) were recovered from metagenomic datasets. The B. longum BFN-121-2 genome encoded more TCAAGCTTGA motifs (4.2 copies per one million genome sequence) than other Bifidobacterium genomes. Additionally, the communities in the stool of controls (C) were also significantly enriched in functions associated with tetrapyrrole biosynthesis compared to those of eczema (E). Our results show distinct immune-modulatory genomic properties of gut microbiota in infants associated with eczema and provide new insights into potential role of gut microbiota in affecting human immune homeostasis.en
dc.description.sponsorshipThis study was supported by MOH’s National Medical Research Council (NMRC/EDG/1057/2011 and NMRC/CIRG/1414/2014). Seungdae Oh was supported by the start-up grant from the University of Illinois at Urbana-Champaign (to WTL) and Nanyang Technological University (to SO), respectively.en
dc.publisherPublic Library of Science (PLoS)en
dc.relation.urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0184955en
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleImmune-modulatory genomic properties differentiate gut microbiota of infants with and without eczemaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPLOS ONEen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Civil Engineering, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.en
dc.contributor.institutionDepartment of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.en
dc.contributor.institutionDepartment of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, United States of America.en
kaust.authorHong, Pei-Yingen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.