In-line balanced detection stimulated Raman scattering microscopy

Handle URI:
http://hdl.handle.net/10754/625800
Title:
In-line balanced detection stimulated Raman scattering microscopy
Authors:
Crisafi, Francesco ( 0000-0002-6577-7967 ) ; Kumar, Vikas; Scopigno, Tullio; Marangoni, Marco; Cerullo, Giulio; Polli, Dario ( 0000-0002-6960-5708 )
Abstract:
We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.
Citation:
Crisafi F, Kumar V, Scopigno T, Marangoni M, Cerullo G, et al. (2017) In-line balanced detection stimulated Raman scattering microscopy. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-09839-1.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
31-Aug-2017
DOI:
10.1038/s41598-017-09839-1
Type:
Article
ISSN:
2045-2322
Sponsors:
This work has been supported by European Research Council Consolidator Grant VIBRA (ERC-2014-CoG 648615), Horizon2020 GRAPHENE Flagship (696656) and KAUST (OSR-2016-CRG5-3017-01). We thank Andrea Ragni for his help in detector noise measurements and calculations. Abigail D.G. Nunn, L. Belloni and N. Pediconi are kindly acknowledged for providing HepaRG and HuH7 cell lines.
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Full metadata record

DC FieldValue Language
dc.contributor.authorCrisafi, Francescoen
dc.contributor.authorKumar, Vikasen
dc.contributor.authorScopigno, Tullioen
dc.contributor.authorMarangoni, Marcoen
dc.contributor.authorCerullo, Giulioen
dc.contributor.authorPolli, Darioen
dc.date.accessioned2017-10-04T14:59:16Z-
dc.date.available2017-10-04T14:59:16Z-
dc.date.issued2017-08-31en
dc.identifier.citationCrisafi F, Kumar V, Scopigno T, Marangoni M, Cerullo G, et al. (2017) In-line balanced detection stimulated Raman scattering microscopy. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-09839-1.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/s41598-017-09839-1en
dc.identifier.urihttp://hdl.handle.net/10754/625800-
dc.description.abstractWe introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.en
dc.description.sponsorshipThis work has been supported by European Research Council Consolidator Grant VIBRA (ERC-2014-CoG 648615), Horizon2020 GRAPHENE Flagship (696656) and KAUST (OSR-2016-CRG5-3017-01). We thank Andrea Ragni for his help in detector noise measurements and calculations. Abigail D.G. Nunn, L. Belloni and N. Pediconi are kindly acknowledged for providing HepaRG and HuH7 cell lines.en
dc.publisherSpringer Natureen
dc.titleIn-line balanced detection stimulated Raman scattering microscopyen
dc.typeArticleen
dc.identifier.journalScientific Reportsen
dc.contributor.institutionIFN-CNR, Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133, Milano, Italyen
dc.contributor.institutionIstituto Italiano di Tecnologia, Center for Life Nano Science @Sapienza, Roma, I-00161, Italyen
dc.contributor.institutionDipartimento di Fisica, Università di Roma “La Sapienza”, Roma, I-00161, Italyen
dc.contributor.institutionCenter for Nano Science and Technology at Polimi, Istituto Italiano di Tecnologia, Milano, 20133, Italyen
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