Assessing the utility of eDNA as a tool to survey reef-fish communities in the Red Sea

Handle URI:
http://hdl.handle.net/10754/625746
Title:
Assessing the utility of eDNA as a tool to survey reef-fish communities in the Red Sea
Authors:
DiBattista, Joseph D. ( 0000-0002-5696-7574 ) ; Coker, Darren J. ( 0000-0002-5099-0984 ) ; Sinclair-Taylor, Tane; Stat, Michael ( 0000-0002-1663-3422 ) ; Berumen, Michael L. ( 0000-0003-2463-2742 ) ; Bunce, Michael ( 0000-0002-0302-4206 )
Abstract:
Relatively small volumes of water may contain sufficient environmental DNA (eDNA) to detect target aquatic organisms via genetic sequencing. We therefore assessed the utility of eDNA to document the diversity of coral reef fishes in the central Red Sea. DNA from seawater samples was extracted, amplified using fish-specific 16S mitochondrial DNA primers, and sequenced using a metabarcoding workflow. DNA sequences were assigned to taxa using available genetic repositories or custom genetic databases generated from reference fishes. Our approach revealed a diversity of conspicuous, cryptobenthic, and commercially relevant reef fish at the genus level, with select genera in the family Labridae over-represented. Our approach, however, failed to capture a significant fraction of the fish fauna known to inhabit the Red Sea, which we attribute to limited spatial sampling, amplification stochasticity, and an apparent lack of sequencing depth. Given an increase in fish species descriptions, completeness of taxonomic checklists, and improvement in species-level assignment with custom genetic databases as shown here, we suggest that the Red Sea region may be ideal for further testing of the eDNA approach.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
DiBattista JD, Coker DJ, Sinclair-Taylor TH, Stat M, Berumen ML, et al. (2017) Assessing the utility of eDNA as a tool to survey reef-fish communities in the Red Sea. Coral Reefs. Available: http://dx.doi.org/10.1007/s00338-017-1618-1.
Publisher:
Springer Nature
Journal:
Coral Reefs
Issue Date:
23-Aug-2017
DOI:
10.1007/s00338-017-1618-1
Type:
Article
ISSN:
0722-4028; 1432-0975
Sponsors:
This study was funded by KAUST Award No. CRG-1-2012-BER-002 and baseline research funds to M.L.B., as well as a Curtin University Early Career Research Fellowship to J.D.D., M.S., and M.B. acknowledge the support of ARC Linkage Project (LP160100839) to explore marine metabarcoding applications. The authors would also like to acknowledge KAUST’s Coastal and Marine Resources Core Lab for logistical support, as well as Matthew Power, Megan Coghlan, and Brendan Chapman for DNA sequencing assistance.
Additional Links:
https://link.springer.com/article/10.1007%2Fs00338-017-1618-1
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorDiBattista, Joseph D.en
dc.contributor.authorCoker, Darren J.en
dc.contributor.authorSinclair-Taylor, Taneen
dc.contributor.authorStat, Michaelen
dc.contributor.authorBerumen, Michael L.en
dc.contributor.authorBunce, Michaelen
dc.date.accessioned2017-10-03T12:49:37Z-
dc.date.available2017-10-03T12:49:37Z-
dc.date.issued2017-08-23en
dc.identifier.citationDiBattista JD, Coker DJ, Sinclair-Taylor TH, Stat M, Berumen ML, et al. (2017) Assessing the utility of eDNA as a tool to survey reef-fish communities in the Red Sea. Coral Reefs. Available: http://dx.doi.org/10.1007/s00338-017-1618-1.en
dc.identifier.issn0722-4028en
dc.identifier.issn1432-0975en
dc.identifier.doi10.1007/s00338-017-1618-1en
dc.identifier.urihttp://hdl.handle.net/10754/625746-
dc.description.abstractRelatively small volumes of water may contain sufficient environmental DNA (eDNA) to detect target aquatic organisms via genetic sequencing. We therefore assessed the utility of eDNA to document the diversity of coral reef fishes in the central Red Sea. DNA from seawater samples was extracted, amplified using fish-specific 16S mitochondrial DNA primers, and sequenced using a metabarcoding workflow. DNA sequences were assigned to taxa using available genetic repositories or custom genetic databases generated from reference fishes. Our approach revealed a diversity of conspicuous, cryptobenthic, and commercially relevant reef fish at the genus level, with select genera in the family Labridae over-represented. Our approach, however, failed to capture a significant fraction of the fish fauna known to inhabit the Red Sea, which we attribute to limited spatial sampling, amplification stochasticity, and an apparent lack of sequencing depth. Given an increase in fish species descriptions, completeness of taxonomic checklists, and improvement in species-level assignment with custom genetic databases as shown here, we suggest that the Red Sea region may be ideal for further testing of the eDNA approach.en
dc.description.sponsorshipThis study was funded by KAUST Award No. CRG-1-2012-BER-002 and baseline research funds to M.L.B., as well as a Curtin University Early Career Research Fellowship to J.D.D., M.S., and M.B. acknowledge the support of ARC Linkage Project (LP160100839) to explore marine metabarcoding applications. The authors would also like to acknowledge KAUST’s Coastal and Marine Resources Core Lab for logistical support, as well as Matthew Power, Megan Coghlan, and Brendan Chapman for DNA sequencing assistance.en
dc.publisherSpringer Natureen
dc.relation.urlhttps://link.springer.com/article/10.1007%2Fs00338-017-1618-1en
dc.subjectBiodiversityen
dc.subjectCoral reefen
dc.subjectEnvironmental DNAen
dc.subjectMetabarcodingen
dc.subjectNext-generation sequencingen
dc.titleAssessing the utility of eDNA as a tool to survey reef-fish communities in the Red Seaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalCoral Reefsen
dc.contributor.institutionTrace and Environmental DNA Laboratory, Department of Environment and Agriculture, Curtin University, Perth, Australiaen
kaust.authorCoker, Darren J.en
kaust.authorSinclair-Taylor, Taneen
kaust.authorBerumen, Michael L.en
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