Ocean acidification responses in paralarval squid swimming behavior using a novel 3D tracking system

Handle URI:
http://hdl.handle.net/10754/625744
Title:
Ocean acidification responses in paralarval squid swimming behavior using a novel 3D tracking system
Authors:
Zakroff, Casey ( 0000-0001-6979-1857 ) ; Mooney, T. Aran; Wirth, Colin
Abstract:
Chronic embryonic exposure to ocean acidification (OA) has been shown to degrade the aragonitic statolith of paralarval squid, Doryteuthis pealeii, a key structure for their swimming behavior. This study examined if day-of-hatching paralarval D. pealeii from eggs reared under chronic OA demonstrated measurable impairments to swimming activity and control. This required the development of a novel, cost-effective, and robust method for 3D motion tracking and analysis. Squid eggs were reared in pCO2 levels in a dose-dependent manner ranging from 400 to 2200 ppm. Initial 2D experiments showed paralarvae in higher acidification environments spent more time at depth. In 3D experiments, velocity, particularly positive and negative vertical velocities, significantly decreased from 400 to 1000 ppm pCO2, but showed non-significant decreases at higher concentrations. Activity and horizontal velocity decreased linearly with increasing pCO2, indicating a subtle impact to paralarval energetics. Patterns may have been obscured by notable individual variability in the paralarvae. Responses were also seen to vary between trials on cohort or potentially annual scales. Overall, paralarval swimming appeared resilient to OA, with effects being slight. The newly developed 3D tracking system provides a powerful and accessible method for future studies to explore similar questions in the larvae of aquatic taxa.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Zakroff C, Mooney TA, Wirth C (2017) Ocean acidification responses in paralarval squid swimming behavior using a novel 3D tracking system. Hydrobiologia. Available: http://dx.doi.org/10.1007/s10750-017-3342-9.
Publisher:
Springer Nature
Journal:
Hydrobiologia
Issue Date:
22-Aug-2017
DOI:
10.1007/s10750-017-3342-9
Type:
Article
ISSN:
0018-8158; 1573-5117
Sponsors:
We thank D. Remsen, the MBL Marine Resources Center staff, and MBL Gemma crew for their support in acquiring squid. R. Galat and the facilities staff of the WHOI ESL provided system support. D. McCorkle, KYK Chan, and M. White provided valuable insight on the OA system. E. Moberg, A. Beet, and A. Solow assisted in the development and coding of the 3D model system. We also thank E. Bonk, K. Hoering, M. Lee, D. Weiler, and A. Schlunk for their assistance and input with the experiments. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. 1122374. This project is funded by NSF Grant No. 1220034.
Additional Links:
https://link.springer.com/article/10.1007%2Fs10750-017-3342-9
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorZakroff, Caseyen
dc.contributor.authorMooney, T. Aranen
dc.contributor.authorWirth, Colinen
dc.date.accessioned2017-10-03T12:49:37Z-
dc.date.available2017-10-03T12:49:37Z-
dc.date.issued2017-08-22en
dc.identifier.citationZakroff C, Mooney TA, Wirth C (2017) Ocean acidification responses in paralarval squid swimming behavior using a novel 3D tracking system. Hydrobiologia. Available: http://dx.doi.org/10.1007/s10750-017-3342-9.en
dc.identifier.issn0018-8158en
dc.identifier.issn1573-5117en
dc.identifier.doi10.1007/s10750-017-3342-9en
dc.identifier.urihttp://hdl.handle.net/10754/625744-
dc.description.abstractChronic embryonic exposure to ocean acidification (OA) has been shown to degrade the aragonitic statolith of paralarval squid, Doryteuthis pealeii, a key structure for their swimming behavior. This study examined if day-of-hatching paralarval D. pealeii from eggs reared under chronic OA demonstrated measurable impairments to swimming activity and control. This required the development of a novel, cost-effective, and robust method for 3D motion tracking and analysis. Squid eggs were reared in pCO2 levels in a dose-dependent manner ranging from 400 to 2200 ppm. Initial 2D experiments showed paralarvae in higher acidification environments spent more time at depth. In 3D experiments, velocity, particularly positive and negative vertical velocities, significantly decreased from 400 to 1000 ppm pCO2, but showed non-significant decreases at higher concentrations. Activity and horizontal velocity decreased linearly with increasing pCO2, indicating a subtle impact to paralarval energetics. Patterns may have been obscured by notable individual variability in the paralarvae. Responses were also seen to vary between trials on cohort or potentially annual scales. Overall, paralarval swimming appeared resilient to OA, with effects being slight. The newly developed 3D tracking system provides a powerful and accessible method for future studies to explore similar questions in the larvae of aquatic taxa.en
dc.description.sponsorshipWe thank D. Remsen, the MBL Marine Resources Center staff, and MBL Gemma crew for their support in acquiring squid. R. Galat and the facilities staff of the WHOI ESL provided system support. D. McCorkle, KYK Chan, and M. White provided valuable insight on the OA system. E. Moberg, A. Beet, and A. Solow assisted in the development and coding of the 3D model system. We also thank E. Bonk, K. Hoering, M. Lee, D. Weiler, and A. Schlunk for their assistance and input with the experiments. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. 1122374. This project is funded by NSF Grant No. 1220034.en
dc.publisherSpringer Natureen
dc.relation.urlhttps://link.springer.com/article/10.1007%2Fs10750-017-3342-9en
dc.subjectHypercapniaen
dc.subjectCephalopoden
dc.subjectLarvaeen
dc.subjectMovement analysisen
dc.subjectStress physiologyen
dc.titleOcean acidification responses in paralarval squid swimming behavior using a novel 3D tracking systemen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalHydrobiologiaen
dc.contributor.institutionBiology Department, Woods Hole Oceanographic Institution, Woods Hole, USAen
dc.contributor.institutionMassachusetts Institute of Technology-Woods Hole Oceanographic Institution Joint Program in Oceanography/Applied Ocean Science and Engineering, Cambridge, USAen
kaust.authorZakroff, Caseyen
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