Effects of metal contamination on the gene expression profile of two benthic species: Cerastoderma edule and Ruditapes philippinarum

Handle URI:
http://hdl.handle.net/10754/625736
Title:
Effects of metal contamination on the gene expression profile of two benthic species: Cerastoderma edule and Ruditapes philippinarum
Authors:
Rodrigues, Raquel ( 0000-0002-2903-3258 ) ; Lourenço, Joana ( 0000-0003-0301-6385 ) ; Pereira, Patrícia; Carvalho, Susana ( 0000-0002-3033-2765 ) ; Mendo, Sonia
Abstract:
This study aimed to identify new biomarkers for metal exposure in two bivalve species. Suppressive Subtractive Hybridization (SSH) was employed to evaluate the transcriptomic response of Cerastoderma edule and Ruditapes philippinarum to metal pollution. Protein synthesis and catalytic activity were the most affected metabolic processes in C. edule and R. philippinarum, respectively. Also, different genes responded to the effect of contamination in each species. The different response observed in both species reinforces the importance of including more than one bioindicator species in risk assessment studies. These results provide the basis for new studies, which are necessary for further validation of the use of the identified genes as molecular biomarkers for metal exposure.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Rodrigues R, Lourenço J, Pereira P, Carvalho S, Mendo S (2017) Effects of metal contamination on the gene expression profile of two benthic species: Cerastoderma edule and Ruditapes philippinarum. Marine Pollution Bulletin. Available: http://dx.doi.org/10.1016/j.marpolbul.2017.08.014.
Publisher:
Elsevier BV
Journal:
Marine Pollution Bulletin
Issue Date:
14-Aug-2017
DOI:
10.1016/j.marpolbul.2017.08.014
Type:
Article
ISSN:
0025-326X
Sponsors:
This work was funded by the “Fundação para a Ciência e a Tecnologia” (FCT; Government of Portugal) through the Research Project PTDC/AAC-AMB/121037/2010 [co-financed by FCT/MCTES in its national budget component (PIDDAC) through the Investigation Fellowship (BI/CESAM/PTDC/AAC-AMB/121037/2010), by the European Regional Development Fund (ERDF) through COMPETE—Thematic Factors of Competitiveness Operational Program (POFC)], and the Post-doctoral fellowships SFRH/BPD/69563/2010 and SFRH/BPD/92554/2013.
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0025326X17306847; http://www.sciencedirect.com/science/article/pii/S0025326X17306847
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorRodrigues, Raquelen
dc.contributor.authorLourenço, Joanaen
dc.contributor.authorPereira, Patríciaen
dc.contributor.authorCarvalho, Susanaen
dc.contributor.authorMendo, Soniaen
dc.date.accessioned2017-10-03T12:49:36Z-
dc.date.available2017-10-03T12:49:36Z-
dc.date.issued2017-08-14en
dc.identifier.citationRodrigues R, Lourenço J, Pereira P, Carvalho S, Mendo S (2017) Effects of metal contamination on the gene expression profile of two benthic species: Cerastoderma edule and Ruditapes philippinarum. Marine Pollution Bulletin. Available: http://dx.doi.org/10.1016/j.marpolbul.2017.08.014.en
dc.identifier.issn0025-326Xen
dc.identifier.doi10.1016/j.marpolbul.2017.08.014en
dc.identifier.urihttp://hdl.handle.net/10754/625736-
dc.description.abstractThis study aimed to identify new biomarkers for metal exposure in two bivalve species. Suppressive Subtractive Hybridization (SSH) was employed to evaluate the transcriptomic response of Cerastoderma edule and Ruditapes philippinarum to metal pollution. Protein synthesis and catalytic activity were the most affected metabolic processes in C. edule and R. philippinarum, respectively. Also, different genes responded to the effect of contamination in each species. The different response observed in both species reinforces the importance of including more than one bioindicator species in risk assessment studies. These results provide the basis for new studies, which are necessary for further validation of the use of the identified genes as molecular biomarkers for metal exposure.en
dc.description.sponsorshipThis work was funded by the “Fundação para a Ciência e a Tecnologia” (FCT; Government of Portugal) through the Research Project PTDC/AAC-AMB/121037/2010 [co-financed by FCT/MCTES in its national budget component (PIDDAC) through the Investigation Fellowship (BI/CESAM/PTDC/AAC-AMB/121037/2010), by the European Regional Development Fund (ERDF) through COMPETE—Thematic Factors of Competitiveness Operational Program (POFC)], and the Post-doctoral fellowships SFRH/BPD/69563/2010 and SFRH/BPD/92554/2013.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0025326X17306847en
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0025326X17306847en
dc.subjectSuppressive Subtractive Hybridization (SSH)en
dc.subjectqPCRen
dc.subjectTagus estuaryen
dc.subjectBiomarkersen
dc.subjectEnvironmental pollutionen
dc.subjectRisk assessmenten
dc.titleEffects of metal contamination on the gene expression profile of two benthic species: Cerastoderma edule and Ruditapes philippinarumen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalMarine Pollution Bulletinen
dc.contributor.institutionCentre for Environmental and Marine Studies and Department of Biology (CESAM), University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugalen
dc.contributor.institutionCentre for Environmental and Marine Studies and Department of Biology (CESAM), University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.en
dc.contributor.institutionIPMA – Portuguese Institute for the Sea and Atmosphere, Avenida Brasília, 1449-006 Lisbon, Portugalen
dc.contributor.institutionIPMA - Portuguese Institute for the Sea and Atmosphere, Avenida Brasília, 1449-006 Lisbon, Portugal.en
kaust.authorCarvalho, Susanaen
kaust.authorCarvalho, Susanaen
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