Higher degradation of L-Cys by O-acetylserine-thiolyases in Sarcocornia than Salicornia

Handle URI:
http://hdl.handle.net/10754/625704
Title:
Higher degradation of L-Cys by O-acetylserine-thiolyases in Sarcocornia than Salicornia
Authors:
Kurmanbayeva, Assylay; Bekturova, Aizat; Srivastava, Sudhakar; Soltabayeva, Aigerim; Khan, Mohammad Suhail; Salazar, Octavio; Fedoroff, Nina V.; Asatryan, Armine; Ventura, Yvonne; Sagi, Moshe
Abstract:
Salicornia and Sarcocornia are almost identical halophytes whose edible succulent shoots hold promise for commercial production in saline water. Enhanced sulfur nutrition may be beneficial to crops naturally grown on high sulfate. However, little is known about sulfate nutrition in halophytes. Here we show that Salicornia europaea (ecotype RN) exhibits a significant increase in biomass and organic-S accumulation in response to supplemental sulfate, while Sarcocornia fruticosa (ecotype VM) does not, instead exhibiting increased sulfate accumulation. We investigated the role of two pathways on organic-S and biomass accumulation in Salicornia and Sarcoconia: the sulfate reductive pathway that generates cysteine and L-cysteine desulfhydrase that degrades cysteine to H2S, NH3 and pyruvate. The major function of O-acetylserine-(thiol) lyase (OAS-TL; EC 2.5.1.47) is the formation of L-cysteine, but our study shows that the OAS-TL A and B of both halophytes are enzymes that also degrade L-cysteine to H2S. This activity was significantly higher in Sarcocornia than in Salicornia, especially upon sulfate supplementation. The activity of the sulfate reductive pathway key enzyme, adenosine 5'-phosphosulfate reductase (APR, EC 1.8.99.2), was significantly higher in Salicornia than in Sarcocornia. These results suggest that the low organic-S level in Sarcocornia is the result of high L-cysteine degradation rate by OAS-TLs, whereas, the greater organic-S and biomass accumulation in Salicornia is the result of higher APR activity and low L-cysteine degradation rate, resulting in higher net cysteine biosynthesis. These results present an initial road map for halophyte growers to attain better growth rates and nutritional value of Salicornia and Sarcocornia.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; King Abdullah University of Science and Technology (KAUST) CITY: Thuwal Saudi Arabia [SA].
Citation:
Kurmanbayeva A, Bekturova A, Srivastava S, Soltabayeva A, Asatryan A, et al. (2017) Higher Novel L-Cys Degradation Activity Results in Lower Organic-S and Biomass in Sarcocornia than the Related Saltwort, Salicornia . Plant Physiology 175: 272–289. Available: http://dx.doi.org/10.1104/pp.17.00780.
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
Plant Physiology
Issue Date:
26-Jul-2017
DOI:
10.1104/pp.17.00780
Type:
Article
ISSN:
0032-0889; 1532-2548
Sponsors:
We thank Dr. Dominic Standing and Ms.Talya Samani for their technical support and Prof. S. Kopriva (University of Cologne, Köln) for providing OAS-TL A and B antibodies.
Additional Links:
http://www.plantphysiol.org/content/early/2017/07/25/pp.17.00780; http://www.plantphysiol.org/content/early/2017/07/25/pp.17.00780
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorKurmanbayeva, Assylayen
dc.contributor.authorBekturova, Aizaten
dc.contributor.authorSrivastava, Sudhakaren
dc.contributor.authorSoltabayeva, Aigerimen
dc.contributor.authorKhan, Mohammad Suhailen
dc.contributor.authorSalazar, Octavioen
dc.contributor.authorFedoroff, Nina V.en
dc.contributor.authorAsatryan, Armineen
dc.contributor.authorVentura, Yvonneen
dc.contributor.authorSagi, Mosheen
dc.date.accessioned2017-10-03T12:49:35Z-
dc.date.available2017-10-03T12:49:35Z-
dc.date.issued2017-07-26en
dc.identifier.citationKurmanbayeva A, Bekturova A, Srivastava S, Soltabayeva A, Asatryan A, et al. (2017) Higher Novel L-Cys Degradation Activity Results in Lower Organic-S and Biomass in Sarcocornia than the Related Saltwort, Salicornia . Plant Physiology 175: 272–289. Available: http://dx.doi.org/10.1104/pp.17.00780.en
dc.identifier.issn0032-0889en
dc.identifier.issn1532-2548en
dc.identifier.doi10.1104/pp.17.00780en
dc.identifier.urihttp://hdl.handle.net/10754/625704-
dc.description.abstractSalicornia and Sarcocornia are almost identical halophytes whose edible succulent shoots hold promise for commercial production in saline water. Enhanced sulfur nutrition may be beneficial to crops naturally grown on high sulfate. However, little is known about sulfate nutrition in halophytes. Here we show that Salicornia europaea (ecotype RN) exhibits a significant increase in biomass and organic-S accumulation in response to supplemental sulfate, while Sarcocornia fruticosa (ecotype VM) does not, instead exhibiting increased sulfate accumulation. We investigated the role of two pathways on organic-S and biomass accumulation in Salicornia and Sarcoconia: the sulfate reductive pathway that generates cysteine and L-cysteine desulfhydrase that degrades cysteine to H2S, NH3 and pyruvate. The major function of O-acetylserine-(thiol) lyase (OAS-TL; EC 2.5.1.47) is the formation of L-cysteine, but our study shows that the OAS-TL A and B of both halophytes are enzymes that also degrade L-cysteine to H2S. This activity was significantly higher in Sarcocornia than in Salicornia, especially upon sulfate supplementation. The activity of the sulfate reductive pathway key enzyme, adenosine 5'-phosphosulfate reductase (APR, EC 1.8.99.2), was significantly higher in Salicornia than in Sarcocornia. These results suggest that the low organic-S level in Sarcocornia is the result of high L-cysteine degradation rate by OAS-TLs, whereas, the greater organic-S and biomass accumulation in Salicornia is the result of higher APR activity and low L-cysteine degradation rate, resulting in higher net cysteine biosynthesis. These results present an initial road map for halophyte growers to attain better growth rates and nutritional value of Salicornia and Sarcocornia.en
dc.description.sponsorshipWe thank Dr. Dominic Standing and Ms.Talya Samani for their technical support and Prof. S. Kopriva (University of Cologne, Köln) for providing OAS-TL A and B antibodies.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.plantphysiol.org/content/early/2017/07/25/pp.17.00780en
dc.relation.urlhttp://www.plantphysiol.org/content/early/2017/07/25/pp.17.00780en
dc.titleHigher degradation of L-Cys by O-acetylserine-thiolyases in Sarcocornia than Salicorniaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentKing Abdullah University of Science and Technology (KAUST) CITY: Thuwal Saudi Arabia [SA].en
dc.identifier.journalPlant Physiologyen
dc.contributor.institutionBen-Gurion University CITY: Beer-Sheva Israel [IL].en
dc.contributor.institutionPlant Stress laboratory, French Associates Institute for Agriculture and Biotechnology of Drylands, Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus 84990, Israelen
dc.contributor.institutionEvan Pugh Professor Emerita, Penn State University, University Park, PA, USAen
dc.contributor.institutionOFW Law CITY: Washington STATE: DC POSTAL_CODE: 20037 United States Of America [US].en
dc.contributor.institutionBen-Gurion University CITY: Beer-Sheva POSTAL_CODE: 84105 Israel [IL] gizi@bgu.ac.il.en
kaust.authorKhan, Mohammad Suhailen
kaust.authorKhan, Mohammad Suhailen
kaust.authorSalazar, Octavioen
kaust.authorSalazar, Octavioen
kaust.authorFedoroff, Nina V.en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.