Varying occurrence of extended-spectrum beta-lactamase bacteria among three produce types

Handle URI:
http://hdl.handle.net/10754/625668
Title:
Varying occurrence of extended-spectrum beta-lactamase bacteria among three produce types
Authors:
Toh, Benjamin E. W.; Bokhari, Osama Mohammed ( 0000-0002-4364-360X ) ; Kutbi, Abdullah; Haroon, Mohamed; Mantilla-Calderon, David; Zowawi, Hosam; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
A monitoring effort that spanned across 1.5 years was conducted to examine three types of produce-associated microbiota. The average amount of antibiotic-resistant bacteria recovered from lettuce, tomato, and cucumber was 1.02 × 1010, 2.05 × 107, and 4.78 × 109 cells per 50 g of each produce, respectively. A total of 480 bacterial isolates were obtained and identified from their 16S rRNA genes, revealing isolates that were ubiquitously recovered from all three types of produce. However, sporadic presence of Klebsiella pneumoniae and Acinetobacter baumannii was detected on lettuce and cucumbers but not tomatoes. End-point PCR revealed that the K. pneumoniae and A. baumannii isolates were positive for genes encoding extended spectrum beta-lactamase. Whole genome sequencing of two of the K. pneumoniae isolates further suggested the presence of the blaCTX-M-15 gene in a conjugative plasmid, as well as other antibiotic resistance genes and virulence-associated traits in either conjugative plasmids or the chromosomal genome. Quantitative microbial risk assessment indicated varying levels of ingestion risk associated with different types of produce. In particular, the risk arising from ESBL-positive K. pneumoniae in lettuce, but not in cucumbers or tomatoes, was higher than the acceptable annual risk of 10−4. Practical applications Three types of vegetables were sampled and evaluated over 1.5 years to determine differences in their associated bacterial isolates. Particular emphasis was placed on identifying pathogenic strains that were positive for extended spectrum beta-lactamase (ESBL). Quantitative estimates of the microbial risk associated with the ESBL-positive pathogens showed that different produce types may incur varying levels of ingestion risk. Most of the currently reported ESBL-positive bacterial isolates have been identified in nosocomial environments. However, the carriage of such drug-resistant bacteria in vegetables suggests a possible connection between our daily diet and human health.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Environmental Science and Engineering Program; Red Sea Research Center (RSRC); Water Desalination and Reuse Research Center (WDRC)
Citation:
Toh BEW, Bokhari O, Kutbi A, Haroon MF, Mantilla-Calderon D, et al. (2017) Varying occurrence of extended-spectrum beta-lactamase bacteria among three produce types. Journal of Food Safety: e12373. Available: http://dx.doi.org/10.1111/jfs.12373.
Publisher:
Wiley-Blackwell
Journal:
Journal of Food Safety
KAUST Grant Number:
BAS/1/1033–01-01
Issue Date:
7-Jul-2017
DOI:
10.1111/jfs.12373
Type:
Article
ISSN:
0149-6085
Sponsors:
The authors would like to thank Ms. Alaa Ragab and the students of EnSE314 Public Health Microbiology Spring 2015 for processing some of the samples. The authors would also like to thank the anonymous respondents who took part in the survey that provided information on dietary consumption rates of the different produce. This work is funded by KAUST Baseline funding BAS/1/1033–01-01 awarded to P.-Y. Hong.
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/jfs.12373/full
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorToh, Benjamin E. W.en
dc.contributor.authorBokhari, Osama Mohammeden
dc.contributor.authorKutbi, Abdullahen
dc.contributor.authorHaroon, Mohameden
dc.contributor.authorMantilla-Calderon, Daviden
dc.contributor.authorZowawi, Hosamen
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2017-10-03T12:49:32Z-
dc.date.available2017-10-03T12:49:32Z-
dc.date.issued2017-07-07en
dc.identifier.citationToh BEW, Bokhari O, Kutbi A, Haroon MF, Mantilla-Calderon D, et al. (2017) Varying occurrence of extended-spectrum beta-lactamase bacteria among three produce types. Journal of Food Safety: e12373. Available: http://dx.doi.org/10.1111/jfs.12373.en
dc.identifier.issn0149-6085en
dc.identifier.doi10.1111/jfs.12373en
dc.identifier.urihttp://hdl.handle.net/10754/625668-
dc.description.abstractA monitoring effort that spanned across 1.5 years was conducted to examine three types of produce-associated microbiota. The average amount of antibiotic-resistant bacteria recovered from lettuce, tomato, and cucumber was 1.02 × 1010, 2.05 × 107, and 4.78 × 109 cells per 50 g of each produce, respectively. A total of 480 bacterial isolates were obtained and identified from their 16S rRNA genes, revealing isolates that were ubiquitously recovered from all three types of produce. However, sporadic presence of Klebsiella pneumoniae and Acinetobacter baumannii was detected on lettuce and cucumbers but not tomatoes. End-point PCR revealed that the K. pneumoniae and A. baumannii isolates were positive for genes encoding extended spectrum beta-lactamase. Whole genome sequencing of two of the K. pneumoniae isolates further suggested the presence of the blaCTX-M-15 gene in a conjugative plasmid, as well as other antibiotic resistance genes and virulence-associated traits in either conjugative plasmids or the chromosomal genome. Quantitative microbial risk assessment indicated varying levels of ingestion risk associated with different types of produce. In particular, the risk arising from ESBL-positive K. pneumoniae in lettuce, but not in cucumbers or tomatoes, was higher than the acceptable annual risk of 10−4. Practical applications Three types of vegetables were sampled and evaluated over 1.5 years to determine differences in their associated bacterial isolates. Particular emphasis was placed on identifying pathogenic strains that were positive for extended spectrum beta-lactamase (ESBL). Quantitative estimates of the microbial risk associated with the ESBL-positive pathogens showed that different produce types may incur varying levels of ingestion risk. Most of the currently reported ESBL-positive bacterial isolates have been identified in nosocomial environments. However, the carriage of such drug-resistant bacteria in vegetables suggests a possible connection between our daily diet and human health.en
dc.description.sponsorshipThe authors would like to thank Ms. Alaa Ragab and the students of EnSE314 Public Health Microbiology Spring 2015 for processing some of the samples. The authors would also like to thank the anonymous respondents who took part in the survey that provided information on dietary consumption rates of the different produce. This work is funded by KAUST Baseline funding BAS/1/1033–01-01 awarded to P.-Y. Hong.en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/jfs.12373/fullen
dc.rightsThis is the peer reviewed version of the following article: Varying occurrence of extended-spectrum beta-lactamase bacteria among three produce types, which has been published in final form at http://doi.org/10.1111/jfs.12373. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.titleVarying occurrence of extended-spectrum beta-lactamase bacteria among three produce typesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalJournal of Food Safetyen
dc.eprint.versionPost-printen
dc.contributor.institutionWorld Health Organization Collaborating Centre for Infection Prevention and Control and Gulf Cooperation Council Center for Infection Control; Riyadh Saudi Arabiaen
dc.contributor.institutionKing Saud bin Abdulaziz University for Health Sciences; Riyadh Saudi Arabiaen
dc.contributor.institutionUQ Centre for Clinical Research; The University of Queensland, Queensland, Royal Brisbane and Women's Hospital Campus; Brisbane Australiaen
kaust.authorToh, Benjamin E. W.en
kaust.authorBokhari, Osama Mohammeden
kaust.authorKutbi, Abdullahen
kaust.authorHaroon, Mohameden
kaust.authorMantilla-Calderon, Daviden
kaust.authorHong, Pei-Yingen
kaust.grant.numberBAS/1/1033–01-01en
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