Fabrication of highly modulable fibrous 3D extracellular microenvironments

Handle URI:
http://hdl.handle.net/10754/625609
Title:
Fabrication of highly modulable fibrous 3D extracellular microenvironments
Authors:
Zhang, Xixiang ( 0000-0002-3478-6414 ) ; Han, Fangfei; Syed, Ahad; Bukhari, Ebtihaj M.; Siang, Basil Chew Joo; Yang, Shan; Zhou, Bingpu; Wen, Wei-jia; Jiang, Dechen
Abstract:
Three-dimensional (3D) in vitro scaffolds that mimic the irregular fibrous structures of in vivo extracellular matrix (ECM) are critical for many important biological applications. However, structural properties modulation of fibrous 3D scaffolds remains a challenge. Here, we report the first highly modulable 3D fibrous scaffolds self-assembled by high-aspect-ratio (HAR) microfibers. The scaffolds structural properties can be easily tailored to incorporate various physical cues, including geometry, stiffness, heterogeneity and nanotopography. Moreover, the fibrous scaffolds are readily and accurately patterned on desired locations of the substrate. Cell culture exhibits that our scaffolds can elicit strong bidirectional cell-material interactions. Furthermore, a functional disparity between the two-dimensional substrate and our 3D scaffolds is identified by cell spreading and proliferation data. These results prove the potential of the proposed scaffold as a biomimetic extracellular microenvironment for cell study.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Imaging & Characterization Core Lab, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Kingdom of Saudi Arabia.; Advanced Nanofabrication and Thin Film Core Lab
Citation:
Zhang X, Han F, Syed A, Bukhari EM, Siang BCJ, et al. (2017) Fabrication of highly modulable fibrous 3D extracellular microenvironments. Biomedical Microdevices 19. Available: http://dx.doi.org/10.1007/s10544-017-0187-y.
Publisher:
Springer Nature
Journal:
Biomedical Microdevices
Issue Date:
13-Jun-2017
DOI:
10.1007/s10544-017-0187-y
PubMed ID:
28608128
Type:
Article
ISSN:
1387-2176; 1572-8781
Additional Links:
https://link.springer.com/article/10.1007%2Fs10544-017-0187-y
Appears in Collections:
Articles; Advanced Nanofabrication, Imaging and Characterization Core Lab; Physical Sciences and Engineering (PSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Xixiangen
dc.contributor.authorHan, Fangfeien
dc.contributor.authorSyed, Ahaden
dc.contributor.authorBukhari, Ebtihaj M.en
dc.contributor.authorSiang, Basil Chew Jooen
dc.contributor.authorYang, Shanen
dc.contributor.authorZhou, Bingpuen
dc.contributor.authorWen, Wei-jiaen
dc.contributor.authorJiang, Dechenen
dc.date.accessioned2017-10-03T12:49:29Z-
dc.date.available2017-10-03T12:49:29Z-
dc.date.issued2017-06-13en
dc.identifier.citationZhang X, Han F, Syed A, Bukhari EM, Siang BCJ, et al. (2017) Fabrication of highly modulable fibrous 3D extracellular microenvironments. Biomedical Microdevices 19. Available: http://dx.doi.org/10.1007/s10544-017-0187-y.en
dc.identifier.issn1387-2176en
dc.identifier.issn1572-8781en
dc.identifier.pmid28608128-
dc.identifier.doi10.1007/s10544-017-0187-yen
dc.identifier.urihttp://hdl.handle.net/10754/625609-
dc.description.abstractThree-dimensional (3D) in vitro scaffolds that mimic the irregular fibrous structures of in vivo extracellular matrix (ECM) are critical for many important biological applications. However, structural properties modulation of fibrous 3D scaffolds remains a challenge. Here, we report the first highly modulable 3D fibrous scaffolds self-assembled by high-aspect-ratio (HAR) microfibers. The scaffolds structural properties can be easily tailored to incorporate various physical cues, including geometry, stiffness, heterogeneity and nanotopography. Moreover, the fibrous scaffolds are readily and accurately patterned on desired locations of the substrate. Cell culture exhibits that our scaffolds can elicit strong bidirectional cell-material interactions. Furthermore, a functional disparity between the two-dimensional substrate and our 3D scaffolds is identified by cell spreading and proliferation data. These results prove the potential of the proposed scaffold as a biomimetic extracellular microenvironment for cell study.en
dc.publisherSpringer Natureen
dc.relation.urlhttps://link.springer.com/article/10.1007%2Fs10544-017-0187-yen
dc.subjectPDMSen
dc.subjectElectrospinningen
dc.subjectFibrous Scaffoldsen
dc.subject3D Cell Cultureen
dc.subject3D Scaffoldsen
dc.titleFabrication of highly modulable fibrous 3D extracellular microenvironmentsen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentImaging & Characterization Core Lab, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Kingdom of Saudi Arabia.en
dc.contributor.departmentAdvanced Nanofabrication and Thin Film Core Laben
dc.identifier.journalBiomedical Microdevicesen
dc.contributor.institutionState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093, People's Republic of China.en
dc.contributor.institutionDepartment of Physics, Hong Kong University of Science and Technology, Kowloon, Hong Kong, People's Republic of China.en
dc.contributor.institutionState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093, People's Republic of China. dechenjiang@nju.edu.cn.en
kaust.authorZhang, Xixiangen
kaust.authorZhang, Xixiangen
kaust.authorSyed, Ahaden
kaust.authorBukhari, Ebtihaj M.en
kaust.authorSiang, Basil Chew Jooen
kaust.authorYang, Shanen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.