The Mitochondrial DNA (mtDNA)-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination

Handle URI:
http://hdl.handle.net/10754/625577
Title:
The Mitochondrial DNA (mtDNA)-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination
Authors:
Blomme, Jonas; Van Aken, Olivier; Van Leene, Jelle; Jégu, Teddy; De Rycke, Riet Maria; De Bruyne, Michiel; Vercruysse, Jasmien; Nolf, Jonah; Van Daele, Twiggy; De Milde, Liesbeth; Vermeersch, Mattias; Colas des Francs-Small, Catherine; De Jaeger, Geert; Benhamed, Moussa ( 0000-0002-4181-1702 ) ; Millar, A. Harvey; Inzé, Dirk; Gonzalez, Nathalie
Abstract:
In addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial in these organelles to fix damage resulting from endogenous and exogenous factors. Plant organellar genomes are complex compared with their animal counterparts, and although several plant-specific mediators of organelle DNA repair have been reported, many regulators remain to be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) complex B protein, SWIB5, is capable of associating with mitochondrial DNA (mtDNA) in Arabidopsis thaliana. Gainand loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at specific intermediate-sized repeats both under normal and genotoxic conditions. SWIB5 interacts with other mitochondrial SWIB proteins. Gene expression and mutant phenotypic analysis of SWIB5 and SWIB family members suggests a link between organellar genome maintenance and cell proliferation. Taken together, our work presents a protein family that influences mtDNA architecture and homologous recombination in plants and suggests a link between organelle functioning and plant development.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Blomme J, Van Aken O, Van Leene J, Jégu T, De Rycke RM, et al. (2017) The Mitochondrial DNA (mtDNA)-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination. The Plant Cell: tpc.00899.2016. Available: http://dx.doi.org/10.1105/tpc.16.00899.
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
The Plant Cell
Issue Date:
19-Apr-2017
DOI:
10.1105/tpc.16.00899
Type:
Article
ISSN:
1040-4651; 1532-298X
Sponsors:
We thank Annick Bleys for help preparing the manuscript. J.B. thanks the Agency for Innovation by Science and Technology in Flanders (IWT-Vlaanderen) for a predoctoral fellowship(projectno.111164)and the European Molecular Biology Organization for a short-term fellowship (project no. ASTF 605-2014). This work was supported by the Interuniversity Attraction Poles Program (IUAP P7/29 ‘MARS’) initiated by the Belgian Science Policy Office, by Ghent University (‘Bijzonder Onderzoeksfonds Methusalem Project’ no. BOF080/01M00408), and the ARC Centre of Excellence in Plant Energy Biology (CE140100008).
Additional Links:
http://www.plantcell.org/content/29/5/1137
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBlomme, Jonasen
dc.contributor.authorVan Aken, Olivieren
dc.contributor.authorVan Leene, Jelleen
dc.contributor.authorJégu, Teddyen
dc.contributor.authorDe Rycke, Riet Mariaen
dc.contributor.authorDe Bruyne, Michielen
dc.contributor.authorVercruysse, Jasmienen
dc.contributor.authorNolf, Jonahen
dc.contributor.authorVan Daele, Twiggyen
dc.contributor.authorDe Milde, Liesbethen
dc.contributor.authorVermeersch, Mattiasen
dc.contributor.authorColas des Francs-Small, Catherineen
dc.contributor.authorDe Jaeger, Geerten
dc.contributor.authorBenhamed, Moussaen
dc.contributor.authorMillar, A. Harveyen
dc.contributor.authorInzé, Dirken
dc.contributor.authorGonzalez, Nathalieen
dc.date.accessioned2017-10-03T12:49:27Z-
dc.date.available2017-10-03T12:49:27Z-
dc.date.issued2017-04-19en
dc.identifier.citationBlomme J, Van Aken O, Van Leene J, Jégu T, De Rycke RM, et al. (2017) The Mitochondrial DNA (mtDNA)-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination. The Plant Cell: tpc.00899.2016. Available: http://dx.doi.org/10.1105/tpc.16.00899.en
dc.identifier.issn1040-4651en
dc.identifier.issn1532-298Xen
dc.identifier.doi10.1105/tpc.16.00899en
dc.identifier.urihttp://hdl.handle.net/10754/625577-
dc.description.abstractIn addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial in these organelles to fix damage resulting from endogenous and exogenous factors. Plant organellar genomes are complex compared with their animal counterparts, and although several plant-specific mediators of organelle DNA repair have been reported, many regulators remain to be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) complex B protein, SWIB5, is capable of associating with mitochondrial DNA (mtDNA) in Arabidopsis thaliana. Gainand loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at specific intermediate-sized repeats both under normal and genotoxic conditions. SWIB5 interacts with other mitochondrial SWIB proteins. Gene expression and mutant phenotypic analysis of SWIB5 and SWIB family members suggests a link between organellar genome maintenance and cell proliferation. Taken together, our work presents a protein family that influences mtDNA architecture and homologous recombination in plants and suggests a link between organelle functioning and plant development.en
dc.description.sponsorshipWe thank Annick Bleys for help preparing the manuscript. J.B. thanks the Agency for Innovation by Science and Technology in Flanders (IWT-Vlaanderen) for a predoctoral fellowship(projectno.111164)and the European Molecular Biology Organization for a short-term fellowship (project no. ASTF 605-2014). This work was supported by the Interuniversity Attraction Poles Program (IUAP P7/29 ‘MARS’) initiated by the Belgian Science Policy Office, by Ghent University (‘Bijzonder Onderzoeksfonds Methusalem Project’ no. BOF080/01M00408), and the ARC Centre of Excellence in Plant Energy Biology (CE140100008).en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.plantcell.org/content/29/5/1137en
dc.titleThe Mitochondrial DNA (mtDNA)-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombinationen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalThe Plant Cellen
dc.contributor.institutionVIB Center for Plant Systems Biology, Ghent, 9052, , Belgiumen
dc.contributor.institutionDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, 9052, , Belgiumen
dc.contributor.institutionDepartment of Biology, Lund University, Lund, 226 52, , Swedenen
dc.contributor.institutionARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA, 6009, , Australiaen
dc.contributor.institutionMolecular Biology Department, Simches Research Center, Boston, MA, 02114, , United Statesen
dc.contributor.institutionInstitute of Plant Sciences Paris-Saclay (IPS2), CNRS INRA, University Paris-Sud, Orsay, 91400, , , Franceen
dc.contributor.institutionINRA UMR 1332, Biologie du Fruit et Pathologie, Villenave d’Ornon, CS20032, , , Franceen
kaust.authorBenhamed, Moussaen
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