Plant-specific Histone Deacetylases HDT½ Regulate GIBBERELLIN 2-OXIDASE 2 Expression to Control Arabidopsis Root Meristem Cell Number

Handle URI:
http://hdl.handle.net/10754/625522
Title:
Plant-specific Histone Deacetylases HDT½ Regulate GIBBERELLIN 2-OXIDASE 2 Expression to Control Arabidopsis Root Meristem Cell Number
Authors:
Li, Huchen; Torres-Garcia, Jesus; latrasse, David; Benhamed, Moussa ( 0000-0002-4181-1702 ) ; Schilderink, Stefan; Zhou, Wenkun; Kulikova, Olga; Hirt, Heribert ( 0000-0003-3119-9633 ) ; Bisseling, Ton
Abstract:
Root growth is modulated by environmental factors and depends on cell production in the root meristem (RM). New cells in the meristem are generated by stem cells and transit-amplifying cells, which together determine RM cell number. Transcription factors and chromatin-remodelling factors have been implicated in regulating the switch from stem cells to transit-amplifying cells. Here we show that two Arabidopsis thaliana paralogs encoding plant-specific histone deacetylases, HDT1 and HDT2, regulate a second switch from transit-amplifying cells to expanding cells. Knockdown of HDT½ (hdt1,2i) results in an earlier switch and causes a reduced RM cell number. Our data show that HDT½ negatively regulate the acetylation level of the C19-GIBBERELLIN 2-OXIDASE 2 (GA2ox2) locus and repress the expression of GA2ox2 in the RM and elongation zone. Overexpression of GA2ox2 in the RM phenocopies the hdt1,2i phenotype. Conversely, knockout of GA2ox2 partially rescues the root growth defect of hdt1,2i. These results suggest that by repressing the expression of GA2ox2, HDT½ likely fine-tune gibberellin metabolism and they are crucial for regulating the switch from cell division to expansion to determine RM cell number. We propose that HDT½ function as part of a mechanism that modulates root growth in response to environmental factors.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Plant Science Program; Desert Agriculture Initiative
Citation:
Li H, Torres-Garcia J, latrasse D, Benhamed M, Schilderink S, et al. (2017) Plant-specific Histone Deacetylases HDT½ Regulate GIBBERELLIN 2-OXIDASE 2 Expression to Control Arabidopsis Root Meristem Cell Number. The Plant Cell: tpc.00366.2017. Available: http://dx.doi.org/10.1105/tpc.17.00366.
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
The Plant Cell
Issue Date:
31-Aug-2017
DOI:
10.1105/tpc.17.00366
Type:
Article
ISSN:
1040-4651; 1532-298X
Sponsors:
This research was supported by European Research Council (ERC-2011-AdG-294790) and China Scholarship Council (CSC). We thank Ben Scheres from Plant Developmental Biology, Wageningen University and Research Center for his critical reading of this article, and for the provision of Arabidopsis transgenic GFP reporter lines.
Additional Links:
http://www.plantcell.org/content/early/2017/09/08/tpc.17.00366
Appears in Collections:
Articles; Plant Science Program; Desert Agriculture Initiative; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Huchenen
dc.contributor.authorTorres-Garcia, Jesusen
dc.contributor.authorlatrasse, Daviden
dc.contributor.authorBenhamed, Moussaen
dc.contributor.authorSchilderink, Stefanen
dc.contributor.authorZhou, Wenkunen
dc.contributor.authorKulikova, Olgaen
dc.contributor.authorHirt, Heriberten
dc.contributor.authorBisseling, Tonen
dc.date.accessioned2017-10-01T05:07:27Z-
dc.date.available2017-10-01T05:07:27Z-
dc.date.issued2017-08-31en
dc.identifier.citationLi H, Torres-Garcia J, latrasse D, Benhamed M, Schilderink S, et al. (2017) Plant-specific Histone Deacetylases HDT½ Regulate GIBBERELLIN 2-OXIDASE 2 Expression to Control Arabidopsis Root Meristem Cell Number. The Plant Cell: tpc.00366.2017. Available: http://dx.doi.org/10.1105/tpc.17.00366.en
dc.identifier.issn1040-4651en
dc.identifier.issn1532-298Xen
dc.identifier.doi10.1105/tpc.17.00366en
dc.identifier.urihttp://hdl.handle.net/10754/625522-
dc.description.abstractRoot growth is modulated by environmental factors and depends on cell production in the root meristem (RM). New cells in the meristem are generated by stem cells and transit-amplifying cells, which together determine RM cell number. Transcription factors and chromatin-remodelling factors have been implicated in regulating the switch from stem cells to transit-amplifying cells. Here we show that two Arabidopsis thaliana paralogs encoding plant-specific histone deacetylases, HDT1 and HDT2, regulate a second switch from transit-amplifying cells to expanding cells. Knockdown of HDT½ (hdt1,2i) results in an earlier switch and causes a reduced RM cell number. Our data show that HDT½ negatively regulate the acetylation level of the C19-GIBBERELLIN 2-OXIDASE 2 (GA2ox2) locus and repress the expression of GA2ox2 in the RM and elongation zone. Overexpression of GA2ox2 in the RM phenocopies the hdt1,2i phenotype. Conversely, knockout of GA2ox2 partially rescues the root growth defect of hdt1,2i. These results suggest that by repressing the expression of GA2ox2, HDT½ likely fine-tune gibberellin metabolism and they are crucial for regulating the switch from cell division to expansion to determine RM cell number. We propose that HDT½ function as part of a mechanism that modulates root growth in response to environmental factors.en
dc.description.sponsorshipThis research was supported by European Research Council (ERC-2011-AdG-294790) and China Scholarship Council (CSC). We thank Ben Scheres from Plant Developmental Biology, Wageningen University and Research Center for his critical reading of this article, and for the provision of Arabidopsis transgenic GFP reporter lines.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.plantcell.org/content/early/2017/09/08/tpc.17.00366en
dc.rightsArchived with thanks to The Plant Cell. Under the Open Acess.en
dc.titlePlant-specific Histone Deacetylases HDT½ Regulate GIBBERELLIN 2-OXIDASE 2 Expression to Control Arabidopsis Root Meristem Cell Numberen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentPlant Science Programen
dc.contributor.departmentDesert Agriculture Initiativeen
dc.identifier.journalThe Plant Cellen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Plant Sciences, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlandsen
dc.contributor.institutionInstitut de Biologie des Plantes (IBP), CNRS-Université Paris-Sud 11, UMR 8618, 91405 Orsay cedex, Franceen
dc.contributor.institutionUnité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 - Université d’Evry Val d’Essonne - ERL CNRS 8196 - Saclay Plant Sciences, 2 rue Gaston Crémieux, 91057 Evry, Franceen
dc.contributor.institutionDepartment of Plant Sciences, Plant developmental Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlandsen
kaust.authorBenhamed, Moussaen
kaust.authorHirt, Heriberten
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