Spatio-temporal Characterization of Ligand-Receptor Interactions in Blood Stem-Cell Rolling

Handle URI:
http://hdl.handle.net/10754/625469
Title:
Spatio-temporal Characterization of Ligand-Receptor Interactions in Blood Stem-Cell Rolling
Authors:
Al Alwan, Bader; AbuZineh, Karmen; Merzaban, Jasmeen S. ( 0000-0002-7276-2907 ) ; Habuchi, Satoshi ( 0000-0002-6663-2807 )
Abstract:
One of the most important issues in the research on hematopoietic stem/progenitor cells (HSPCs) is to understand the mechanism of the homing process of these cells to the bone marrow after being transplanted into patients and establish the production of various blood cell types. The HSPCs first come in contact with the endothelial cells. This contact is known as adhesion and occurs through a multi-step paradigm ending with transmigration to the bone marrow niche. The initial step of the homing, tethering and rolling of HSPCs is mediated by P- and E-Selectins expressed on the endothelial cell surface through their interactions with the ligands expressed by HSPCs. Here we developed a novel experimental method to unravel the molecular mechanisms of the selectin-ligands interactions in vitro at the single molecule level by combining microfluidics and single-molecule fluorescence imaging. Our method enables direct visualization of the nanoscale spatiotemporal dynamics of the E-selectin-ligand (PSGL-1) interactions under conditions of shear stress acting on the cells at the molecular level in real time.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Conference/Event name:
15th Methods and Applications in Fluorescence conference
Issue Date:
16-Aug-2017
Type:
Poster
Appears in Collections:
Posters

Full metadata record

DC FieldValue Language
dc.contributor.authorAl Alwan, Baderen
dc.contributor.authorAbuZineh, Karmenen
dc.contributor.authorMerzaban, Jasmeen S.en
dc.contributor.authorHabuchi, Satoshien
dc.date.accessioned2017-09-17T13:41:56Z-
dc.date.available2017-09-17T13:41:56Z-
dc.date.issued2017-08-16-
dc.identifier.urihttp://hdl.handle.net/10754/625469-
dc.description.abstractOne of the most important issues in the research on hematopoietic stem/progenitor cells (HSPCs) is to understand the mechanism of the homing process of these cells to the bone marrow after being transplanted into patients and establish the production of various blood cell types. The HSPCs first come in contact with the endothelial cells. This contact is known as adhesion and occurs through a multi-step paradigm ending with transmigration to the bone marrow niche. The initial step of the homing, tethering and rolling of HSPCs is mediated by P- and E-Selectins expressed on the endothelial cell surface through their interactions with the ligands expressed by HSPCs. Here we developed a novel experimental method to unravel the molecular mechanisms of the selectin-ligands interactions in vitro at the single molecule level by combining microfluidics and single-molecule fluorescence imaging. Our method enables direct visualization of the nanoscale spatiotemporal dynamics of the E-selectin-ligand (PSGL-1) interactions under conditions of shear stress acting on the cells at the molecular level in real time.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleSpatio-temporal Characterization of Ligand-Receptor Interactions in Blood Stem-Cell Rollingen
dc.typePosteren
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.conference.dateSeptember 10-13, 2017en
dc.conference.name15th Methods and Applications in Fluorescence conferenceen
dc.conference.locationBruges, Belgiumen
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