A Nucleus-localized Long Non-Coding RNA Enhances Drought and Salt Stress Tolerance

Handle URI:
http://hdl.handle.net/10754/625446
Title:
A Nucleus-localized Long Non-Coding RNA Enhances Drought and Salt Stress Tolerance
Authors:
Qin, Tao; Zhao, Huayan; Cui, Peng ( 0000-0003-3076-0070 ) ; Albesher, Nour H.; Xiong, Liming ( 0000-0001-8099-0806 )
Abstract:
Long non-coding RNAs (lncRNAs) affect gene expression through a wide range of mechanisms and are considered as important regulators in many essential biological processes. A large number of lncRNA transcripts have been predicted or identified in plants in recent years. However, the biological functions for most of them are still unknown. In this study, we identified an Arabidopsis thaliana lncRNA, Drought induced RNA (DRIR), as a novel positive regulator of plant response to drought and salt stress. DRIR was expressed at a low level under non-stress conditions but can be significantly activated by drought and salt stress as well as by abscisic acid (ABA) treatment. We identified a T-DNA insertion mutant, drirD, which had higher expression of the DRIR gene than the wild type plants. The drirD mutant exhibits increased tolerance to drought and salt stress. Overexpressing DRIR in Arabidopsis also increased tolerance to drought and salt stress of the transgenic plants. The drirD mutant and the overexpressing seedlings are more sensitive to ABA than the wild type in stomata closure and seedling growth. Genome-wide transcriptome analysis demonstrated that the expression of a large number of genes was altered in drirD and the overexpressing plants. These include genes involved in ABA signaling, water transport and other stress-relief processes. Our study reveals a mechanism whereby DRIR regulates plant response to abiotic stress by modulating the expression of a series of genes involved in stress response.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Qin T, Zhao H, Cui P, Albesher N, Xiong L (2017) A Nucleus-localized Long Non-Coding RNA Enhances Drought and Salt Stress Tolerance. Plant Physiology: pp.00574.2017. Available: http://dx.doi.org/10.1104/pp.17.00574.
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
Plant Physiology
Issue Date:
9-Sep-2017
DOI:
10.1104/pp.17.00574
Type:
Article
ISSN:
0032-0889; 1532-2548
Sponsors:
We thank the Arabidopsis Biological Resource Center for providing the T-DNA insertion lines.
Additional Links:
http://www.plantphysiol.org/content/early/2017/09/08/pp.17.00574
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorQin, Taoen
dc.contributor.authorZhao, Huayanen
dc.contributor.authorCui, Pengen
dc.contributor.authorAlbesher, Nour H.en
dc.contributor.authorXiong, Limingen
dc.date.accessioned2017-09-14T06:03:50Z-
dc.date.available2017-09-14T06:03:50Z-
dc.date.issued2017-09-09en
dc.identifier.citationQin T, Zhao H, Cui P, Albesher N, Xiong L (2017) A Nucleus-localized Long Non-Coding RNA Enhances Drought and Salt Stress Tolerance. Plant Physiology: pp.00574.2017. Available: http://dx.doi.org/10.1104/pp.17.00574.en
dc.identifier.issn0032-0889en
dc.identifier.issn1532-2548en
dc.identifier.doi10.1104/pp.17.00574en
dc.identifier.urihttp://hdl.handle.net/10754/625446-
dc.description.abstractLong non-coding RNAs (lncRNAs) affect gene expression through a wide range of mechanisms and are considered as important regulators in many essential biological processes. A large number of lncRNA transcripts have been predicted or identified in plants in recent years. However, the biological functions for most of them are still unknown. In this study, we identified an Arabidopsis thaliana lncRNA, Drought induced RNA (DRIR), as a novel positive regulator of plant response to drought and salt stress. DRIR was expressed at a low level under non-stress conditions but can be significantly activated by drought and salt stress as well as by abscisic acid (ABA) treatment. We identified a T-DNA insertion mutant, drirD, which had higher expression of the DRIR gene than the wild type plants. The drirD mutant exhibits increased tolerance to drought and salt stress. Overexpressing DRIR in Arabidopsis also increased tolerance to drought and salt stress of the transgenic plants. The drirD mutant and the overexpressing seedlings are more sensitive to ABA than the wild type in stomata closure and seedling growth. Genome-wide transcriptome analysis demonstrated that the expression of a large number of genes was altered in drirD and the overexpressing plants. These include genes involved in ABA signaling, water transport and other stress-relief processes. Our study reveals a mechanism whereby DRIR regulates plant response to abiotic stress by modulating the expression of a series of genes involved in stress response.en
dc.description.sponsorshipWe thank the Arabidopsis Biological Resource Center for providing the T-DNA insertion lines.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.plantphysiol.org/content/early/2017/09/08/pp.17.00574en
dc.rightsArchived with thanks to Plant Physiology.en
dc.titleA Nucleus-localized Long Non-Coding RNA Enhances Drought and Salt Stress Toleranceen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPlant Physiologyen
dc.eprint.versionPost-printen
dc.contributor.institutionTexas A&M AgriLife Research Center CITY: Dallas STATE: TX United States Of America [US].en
dc.contributor.institutionWuhan botanical garden, CAS CITY: Wuhan China [CN].en
dc.contributor.institutionTexas A&M AgriLife Research Center CITY: Dallas STATE: Texas POSTAL_CODE: 75252 United States Of America [US].en
kaust.authorZhao, Huayanen
kaust.authorCui, Pengen
kaust.authorAlbesher, Nour H.en
kaust.authorXiong, Limingen
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