Microbial competition among anammox bacteria in nitrite-limited bioreactors

Handle URI:
http://hdl.handle.net/10754/625410
Title:
Microbial competition among anammox bacteria in nitrite-limited bioreactors
Authors:
Zhang, Lei; Narita, Yuko; Gao, Lin; Ali, Muhammad ( 0000-0003-3360-1622 ) ; Oshiki, Mamoru; Ishii, Satoshi; Okabe, Satoshi
Abstract:
Phylogenetically diverse anammox bacteria have been detected in most of anoxic natural and engineered ecosystems and thus regarded as key players in the global nitrogen cycle. However, ecological niche differentiation of anammox bacteria remains unresolved despite its ecological and practical importance. In this study, the microbial competitions for a common substrate (nitrite) among three anammox species (i.e. “Candidatus Brocadia sinica”, “Candidatus Jettenia caeni” and “Candidatus Kuenenia stuttgartiensis”) were systematically investigated in nitrite-limited gel-immobilized column reactors (GICR) and membrane bioreactors (MBRs) under different nitrogen loading rates (NLRs). 16 S rRNA gene-based population dynamics revealed that “Ca. J. caeni” could proliferate only at low NLRs, whereas “Ca. B. sinica” outcompeted other two species at higher NLRs in both types of reactors. Furthermore, FISH analysis revealed that “Ca. J. caeni” was mainly present as spherical microclusters at the inner part (low NO2− environment), whereas “Ca. B. sinica” was present throughout the gel beads and granules. This spatial distribution supports the outcomes of the competition experiments. However, the successful competition of “Ca. J. caeni” at low NLR could not be explained with the Monod model probably due to inaccuracy of kinetic parameters such as half saturation constant (Ks) for nitrite and a difference in the maintenance rate (m). In addition, the growth of “Ca. K. stuttgartiensis” could not be observed in any experimental conditions, suggesting possible unknown factor(s) is missing. Taken together, NLR was one of factors determining ecological niche differentiation of “Ca. B. sinica” and “Ca. J. caeni”.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Zhang L, Narita Y, Gao L, Ali M, Oshiki M, et al. (2017) Microbial competition among anammox bacteria in nitrite-limited bioreactors. Water Research. Available: http://dx.doi.org/10.1016/j.watres.2017.08.052.
Publisher:
Elsevier BV
Journal:
Water Research
Issue Date:
26-Aug-2017
DOI:
10.1016/j.watres.2017.08.052
Type:
Article
ISSN:
0043-1354
Sponsors:
This research was financially supported by Nagase Science Technology Foundation and Institute for Fermentation, Osaka, which were granted to Satoshi Okabe. Lei Zhang was supported partly by the Monbukagakusho Honors Scholarship for Privately Financed International Students offered by the Ministry of Education, Culture, Sports, Science and Technology, Japan. Post-Doctoral Fellowship of Muhammad Ali was sponsored by Graduate school of Engineering, Hokkaido University, Japan.
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0043135417307145
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Leien
dc.contributor.authorNarita, Yukoen
dc.contributor.authorGao, Linen
dc.contributor.authorAli, Muhammaden
dc.contributor.authorOshiki, Mamoruen
dc.contributor.authorIshii, Satoshien
dc.contributor.authorOkabe, Satoshien
dc.date.accessioned2017-08-28T10:28:00Z-
dc.date.available2017-08-28T10:28:00Z-
dc.date.issued2017-08-26en
dc.identifier.citationZhang L, Narita Y, Gao L, Ali M, Oshiki M, et al. (2017) Microbial competition among anammox bacteria in nitrite-limited bioreactors. Water Research. Available: http://dx.doi.org/10.1016/j.watres.2017.08.052.en
dc.identifier.issn0043-1354en
dc.identifier.doi10.1016/j.watres.2017.08.052en
dc.identifier.urihttp://hdl.handle.net/10754/625410-
dc.description.abstractPhylogenetically diverse anammox bacteria have been detected in most of anoxic natural and engineered ecosystems and thus regarded as key players in the global nitrogen cycle. However, ecological niche differentiation of anammox bacteria remains unresolved despite its ecological and practical importance. In this study, the microbial competitions for a common substrate (nitrite) among three anammox species (i.e. “Candidatus Brocadia sinica”, “Candidatus Jettenia caeni” and “Candidatus Kuenenia stuttgartiensis”) were systematically investigated in nitrite-limited gel-immobilized column reactors (GICR) and membrane bioreactors (MBRs) under different nitrogen loading rates (NLRs). 16 S rRNA gene-based population dynamics revealed that “Ca. J. caeni” could proliferate only at low NLRs, whereas “Ca. B. sinica” outcompeted other two species at higher NLRs in both types of reactors. Furthermore, FISH analysis revealed that “Ca. J. caeni” was mainly present as spherical microclusters at the inner part (low NO2− environment), whereas “Ca. B. sinica” was present throughout the gel beads and granules. This spatial distribution supports the outcomes of the competition experiments. However, the successful competition of “Ca. J. caeni” at low NLR could not be explained with the Monod model probably due to inaccuracy of kinetic parameters such as half saturation constant (Ks) for nitrite and a difference in the maintenance rate (m). In addition, the growth of “Ca. K. stuttgartiensis” could not be observed in any experimental conditions, suggesting possible unknown factor(s) is missing. Taken together, NLR was one of factors determining ecological niche differentiation of “Ca. B. sinica” and “Ca. J. caeni”.en
dc.description.sponsorshipThis research was financially supported by Nagase Science Technology Foundation and Institute for Fermentation, Osaka, which were granted to Satoshi Okabe. Lei Zhang was supported partly by the Monbukagakusho Honors Scholarship for Privately Financed International Students offered by the Ministry of Education, Culture, Sports, Science and Technology, Japan. Post-Doctoral Fellowship of Muhammad Ali was sponsored by Graduate school of Engineering, Hokkaido University, Japan.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0043135417307145en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Water Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Water Research, 26 August 2017. DOI: 10.1016/j.watres.2017.08.052. © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectNiche differentiationen
dc.subjectPopulation dynamicsen
dc.subjectCa. Brocadia sinicaen
dc.subjectCa. Jettenia caenien
dc.subjectCa. Kuenenia stuttgartiensisen
dc.titleMicrobial competition among anammox bacteria in nitrite-limited bioreactorsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalWater Researchen
dc.eprint.versionPost-printen
dc.contributor.institutionDivision of Environmental Engineering, Faculty of Engineering, Hokkaido University, North 13, West 8, Sapporo, Hokkaido, 060-8628, Japanen
dc.contributor.institutionDepartment of Civil Engineering, Nagaoka National College of Technology, 888 Nishikatakaimachi, Nagaoka, Niigata, 940-0834, Japanen
dc.contributor.institutionDepartment of Soil, Water, and Climate, BioTechnology Institute, University of Minnesota, 140 Gortner Laboratory of BioChemistry, 1479 Gortner Avenue, St. Paul, MN 55108-6106, USAen
kaust.authorAli, Muhammaden
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