Biofouling patterns in spacer filled channels: High resolution imaging for characterization of heterogeneous biofilms

Handle URI:
http://hdl.handle.net/10754/625402
Title:
Biofouling patterns in spacer filled channels: High resolution imaging for characterization of heterogeneous biofilms
Authors:
Staal, Marc; Farhat, Nadia ( 0000-0002-0588-8876 ) ; van Loosdrecht, Mark; Vrouwenvelder, Johannes S. ( 0000-0003-2668-2057 )
Abstract:
Biofilms develop in heterogeneous patterns at a µm scale up to a cm scale, and patterns become more pronounced when biofilms develop under complex hydrodynamic flow regimes. Spatially heterogeneous biofilms are especially known in spiral wound reverse osmosis (RO) and nanofiltration (NF) membrane filtration systems used for desalination and wastewater reuse to produce high quality (drinking) water. These spiral wound membrane modules contain mesh-like spacer structures used to create an intermembrane space and improve water mixing. Spacers create inhomogeneous water flow patterns resulting in zones favouring biofilm growth, possibly leading to biofouling thus hampering water production. Oxygen sensing planar optodes were used to visualize variations in oxygen decrease rates (ODR). ODR is an indication of biofilm activity. In this study, ODR images of multiple repetitive spacer areas in a membrane fouling simulator were averaged to produce high resolution, low noise ODR images. Averaging 40 individual spacer areas improved the ODR distribution image significantly and allowed comparison of biofilm patterning over a spacer structure at different positions in an RO filter. This method clearly showed that most active biofilm accumulated on and in direct vicinity of the spacer. The averaging method was also used to calculate the deviation of ODR patterning from individual spacer areas to the average ODR pattern, proposing a new approach to determine biofilm spatial heterogeneity. This study showed that the averaging method can be applied and that the improved, averaged ODR images can be used as an analytical, in-situ, non-destructive method to assess and quantify the effect of membrane installation operational parameters or different spacer geometries on biofilm development in spiral wound membrane systems characterized by complex hydrodynamic conditions.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Staal M, Farhat N, van Loosdrecht M, Vrouwenvelder J (2017) Biofouling patterns in spacer filled channels: High resolution imaging for characterization of heterogeneous biofilms. DESALINATION AND WATER TREATMENT 80: 1–10. Available: http://dx.doi.org/10.5004/dwt.2017.20863.
Publisher:
Desalination Publications
Journal:
DESALINATION AND WATER TREATMENT
Issue Date:
15-Aug-2017
DOI:
10.5004/dwt.2017.20863
Type:
Article
Sponsors:
The research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST). The authors would like to thank Sergey Borisov for providing the optode material.
Additional Links:
http://www.deswater.com/DWT_articles/vol_80_papers/80_2017_1.pdf
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorStaal, Marcen
dc.contributor.authorFarhat, Nadiaen
dc.contributor.authorvan Loosdrecht, Marken
dc.contributor.authorVrouwenvelder, Johannes S.en
dc.date.accessioned2017-08-28T10:28:00Z-
dc.date.available2017-08-28T10:28:00Z-
dc.date.issued2017-08-15en
dc.identifier.citationStaal M, Farhat N, van Loosdrecht M, Vrouwenvelder J (2017) Biofouling patterns in spacer filled channels: High resolution imaging for characterization of heterogeneous biofilms. DESALINATION AND WATER TREATMENT 80: 1–10. Available: http://dx.doi.org/10.5004/dwt.2017.20863.en
dc.identifier.doi10.5004/dwt.2017.20863en
dc.identifier.urihttp://hdl.handle.net/10754/625402-
dc.description.abstractBiofilms develop in heterogeneous patterns at a µm scale up to a cm scale, and patterns become more pronounced when biofilms develop under complex hydrodynamic flow regimes. Spatially heterogeneous biofilms are especially known in spiral wound reverse osmosis (RO) and nanofiltration (NF) membrane filtration systems used for desalination and wastewater reuse to produce high quality (drinking) water. These spiral wound membrane modules contain mesh-like spacer structures used to create an intermembrane space and improve water mixing. Spacers create inhomogeneous water flow patterns resulting in zones favouring biofilm growth, possibly leading to biofouling thus hampering water production. Oxygen sensing planar optodes were used to visualize variations in oxygen decrease rates (ODR). ODR is an indication of biofilm activity. In this study, ODR images of multiple repetitive spacer areas in a membrane fouling simulator were averaged to produce high resolution, low noise ODR images. Averaging 40 individual spacer areas improved the ODR distribution image significantly and allowed comparison of biofilm patterning over a spacer structure at different positions in an RO filter. This method clearly showed that most active biofilm accumulated on and in direct vicinity of the spacer. The averaging method was also used to calculate the deviation of ODR patterning from individual spacer areas to the average ODR pattern, proposing a new approach to determine biofilm spatial heterogeneity. This study showed that the averaging method can be applied and that the improved, averaged ODR images can be used as an analytical, in-situ, non-destructive method to assess and quantify the effect of membrane installation operational parameters or different spacer geometries on biofilm development in spiral wound membrane systems characterized by complex hydrodynamic conditions.en
dc.description.sponsorshipThe research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST). The authors would like to thank Sergey Borisov for providing the optode material.en
dc.publisherDesalination Publicationsen
dc.relation.urlhttp://www.deswater.com/DWT_articles/vol_80_papers/80_2017_1.pdfen
dc.rightsCopyright is retained by Balaban Publishers – Desalination Publications and is granted to authors who may place their papers on their personal or institutional websites (Harvard System). An electronic copy of the final paper will be provided to authors for such use.en
dc.subjectBiofoulingen
dc.subjectDesalinationen
dc.subjectFeed spaceren
dc.subjectNon-destructiveen
dc.subjectSensoren
dc.subjectSpiral wounden
dc.titleBiofouling patterns in spacer filled channels: High resolution imaging for characterization of heterogeneous biofilmsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalDESALINATION AND WATER TREATMENTen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Biotechnology, Faculty of Applied Sciences, Delft University of Technology, Van der Maasweg 9, Delft, 2629 HZ, , Netherlandsen
kaust.authorFarhat, Nadiaen
kaust.authorVrouwenvelder, Johannes S.en
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