A Biosensor-CMOS Platform and Integrated Readout Circuit in 0.18-μm CMOS Technology for Cancer Biomarker Detection

Handle URI:
http://hdl.handle.net/10754/625399
Title:
A Biosensor-CMOS Platform and Integrated Readout Circuit in 0.18-μm CMOS Technology for Cancer Biomarker Detection
Authors:
Alhoshany, Abdulaziz; Sivashankar, Shilpa ( 0000-0002-2482-2629 ) ; Mashraei, Yousof; Omran, Hesham; Salama, Khaled N. ( 0000-0001-7742-1282 )
Abstract:
This paper presents a biosensor-CMOS platform for measuring the capacitive coupling of biorecognition elements. The biosensor is designed, fabricated, and tested for the detection and quantification of a protein that reveals the presence of early-stage cancer. For the first time, the spermidine/spermine N1 acetyltransferase (SSAT) enzyme has been screened and quantified on the surface of a capacitive sensor. The sensor surface is treated to immobilize antibodies, and the baseline capacitance of the biosensor is reduced by connecting an array of capacitors in series for fixed exposure area to the analyte. A large sensing area with small baseline capacitance is implemented to achieve a high sensitivity to SSAT enzyme concentrations. The sensed capacitance value is digitized by using a 12-bit highly digital successive-approximation capacitance-to-digital converter that is implemented in a 0.18 μm CMOS technology. The readout circuit operates in the near-subthreshold regime and provides power and area efficient operation. The capacitance range is 16.137 pF with a 4.5 fF absolute resolution, which adequately covers the concentrations of 10 mg/L, 5 mg/L, 2.5 mg/L, and 1.25 mg/L of the SSAT enzyme. The concentrations were selected as a pilot study, and the platform was shown to demonstrate high sensitivity for SSAT enzymes on the surface of the capacitive sensor. The tested prototype demonstrated 42.5 μS of measurement time and a total power consumption of 2.1 μW.
KAUST Department:
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Citation:
Alhoshany A, Sivashankar S, Mashraei Y, Omran H, Salama KN (2017) A Biosensor-CMOS Platform and Integrated Readout Circuit in 0.18-μm CMOS Technology for Cancer Biomarker Detection. Sensors 17: 1942. Available: http://dx.doi.org/10.3390/s17091942.
Publisher:
MDPI AG
Journal:
Sensors
Issue Date:
23-Aug-2017
DOI:
10.3390/s17091942
Type:
Article
ISSN:
1424-8220
Sponsors:
The research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://www.mdpi.com/1424-8220/17/9/1942/htm
Appears in Collections:
Articles; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorAlhoshany, Abdulazizen
dc.contributor.authorSivashankar, Shilpaen
dc.contributor.authorMashraei, Yousofen
dc.contributor.authorOmran, Heshamen
dc.contributor.authorSalama, Khaled N.en
dc.date.accessioned2017-08-28T10:27:59Z-
dc.date.available2017-08-28T10:27:59Z-
dc.date.issued2017-08-23en
dc.identifier.citationAlhoshany A, Sivashankar S, Mashraei Y, Omran H, Salama KN (2017) A Biosensor-CMOS Platform and Integrated Readout Circuit in 0.18-μm CMOS Technology for Cancer Biomarker Detection. Sensors 17: 1942. Available: http://dx.doi.org/10.3390/s17091942.en
dc.identifier.issn1424-8220en
dc.identifier.doi10.3390/s17091942en
dc.identifier.urihttp://hdl.handle.net/10754/625399-
dc.description.abstractThis paper presents a biosensor-CMOS platform for measuring the capacitive coupling of biorecognition elements. The biosensor is designed, fabricated, and tested for the detection and quantification of a protein that reveals the presence of early-stage cancer. For the first time, the spermidine/spermine N1 acetyltransferase (SSAT) enzyme has been screened and quantified on the surface of a capacitive sensor. The sensor surface is treated to immobilize antibodies, and the baseline capacitance of the biosensor is reduced by connecting an array of capacitors in series for fixed exposure area to the analyte. A large sensing area with small baseline capacitance is implemented to achieve a high sensitivity to SSAT enzyme concentrations. The sensed capacitance value is digitized by using a 12-bit highly digital successive-approximation capacitance-to-digital converter that is implemented in a 0.18 μm CMOS technology. The readout circuit operates in the near-subthreshold regime and provides power and area efficient operation. The capacitance range is 16.137 pF with a 4.5 fF absolute resolution, which adequately covers the concentrations of 10 mg/L, 5 mg/L, 2.5 mg/L, and 1.25 mg/L of the SSAT enzyme. The concentrations were selected as a pilot study, and the platform was shown to demonstrate high sensitivity for SSAT enzymes on the surface of the capacitive sensor. The tested prototype demonstrated 42.5 μS of measurement time and a total power consumption of 2.1 μW.en
dc.description.sponsorshipThe research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST).en
dc.publisherMDPI AGen
dc.relation.urlhttp://www.mdpi.com/1424-8220/17/9/1942/htmen
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectbiosensoren
dc.subjectinterdigitated electrodesen
dc.subjectSSAT enzyme diagnostic platformen
dc.subjectcapacitive sensor interfaceen
dc.subjectearly cancer detectionen
dc.subjectCMOSen
dc.titleA Biosensor-CMOS Platform and Integrated Readout Circuit in 0.18-μm CMOS Technology for Cancer Biomarker Detectionen
dc.typeArticleen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalSensorsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Biomedical Engineering, University of Chapel Hill/North Carolina State University, Raleigh, NC 27695, USA.en
dc.contributor.institutionThe Integrated Circuits Lab, Faculty of Engineering, Ain Shams University, Cairo 11535, Egypt.en
kaust.authorAlhoshany, Abdulazizen
kaust.authorMashraei, Yousofen
kaust.authorSalama, Khaled N.en
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