Enrichment and physiological characterization of an anaerobic ammonium-oxidizing bacterium ‘ Candidatus Brocadia sapporoensis’

Handle URI:
http://hdl.handle.net/10754/625364
Title:
Enrichment and physiological characterization of an anaerobic ammonium-oxidizing bacterium ‘ Candidatus Brocadia sapporoensis’
Authors:
Narita, Yuko; Zhang, Lei; Kimura, Zen-ichiro; Ali, Muhammad ( 0000-0003-3360-1622 ) ; Fujii, Takao; Okabe, Satoshi
Abstract:
Anaerobic ammonium-oxidation (anammox) is recognized as an important microbial process in the global nitrogen cycle and wastewater treatment. In this study, we successfully enriched a novel anammox bacterium affiliated with the genus ‘Candidatus Brocadia’ with high purity (>90%) in a membrane bioreactor (MBR). The enriched bacterium was distantly related to the hitherto characterized ‘Ca. Brocadia fulgida’ and ‘Ca. Brocadia sinica’ with 96% and 93% of 16S ribosomal RNA gene sequence identity, respectively. The bacterium exhibited the common structural features of anammox bacteria and the production of hydrazine in the presence of hydroxylamine under anoxic conditions. The temperature range of anammox activity was 20 − 45°C with a maximum activity at 37°C. The maximum specific growth rate (μmax) was determined to be 0.0082h−1 at 37°C, corresponding to a doubling time of 3.5 days. The half-saturation constant (KS) for nitrite was 5±2.5μM. The anammox activity was inhibited by nitrite with 11.6mM representing the 50% inhibitory concentration (IC50) but no significant inhibition was observed in the presence of formate and acetate. The major respiratory quinone was identified to be menaquinone-7 (MK-7). Comparative genome analysis revealed that the anammox bacterium enriched in present study shared nearly half of genes with ‘Ca. Brocadia sinica’ and ‘Ca. Brocadia fulgida’. The bacterium enriched in this study showed all known physiological characteristics of anammox bacteria and can be distinguished from the close relatives by its rRNA gene sequences. Therefore, we proposed the name ‘Ca. Brocadia sapporoensis’ sp. nov.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Narita Y, Zhang L, Kimura Z, Ali M, Fujii T, et al. (2017) Enrichment and physiological characterization of an anaerobic ammonium-oxidizing bacterium “ Candidatus Brocadia sapporoensis.” Systematic and Applied Microbiology. Available: http://dx.doi.org/10.1016/j.syapm.2017.07.004.
Publisher:
Elsevier BV
Journal:
Systematic and Applied Microbiology
Issue Date:
18-Aug-2017
DOI:
10.1016/j.syapm.2017.07.004
Type:
Article
ISSN:
0723-2020
Sponsors:
This research was financially supported by Nagase Science and Technology Foundation and Institute for Fermentation, Osaka (IFO), which were granted to Satoshi Okabe. Lei Zhang was supported partly by the Monbukagakusho Honors Scholarship for Privately Financed International Students offered by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. Post-Doctoral Fellowship of Muhammad Ali was sponsored by Graduate school of Engineering, Hokkaido University, Japan.
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0723202017300929
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorNarita, Yukoen
dc.contributor.authorZhang, Leien
dc.contributor.authorKimura, Zen-ichiroen
dc.contributor.authorAli, Muhammaden
dc.contributor.authorFujii, Takaoen
dc.contributor.authorOkabe, Satoshien
dc.date.accessioned2017-08-21T06:28:04Z-
dc.date.available2017-08-21T06:28:04Z-
dc.date.issued2017-08-18en
dc.identifier.citationNarita Y, Zhang L, Kimura Z, Ali M, Fujii T, et al. (2017) Enrichment and physiological characterization of an anaerobic ammonium-oxidizing bacterium “ Candidatus Brocadia sapporoensis.” Systematic and Applied Microbiology. Available: http://dx.doi.org/10.1016/j.syapm.2017.07.004.en
dc.identifier.issn0723-2020en
dc.identifier.doi10.1016/j.syapm.2017.07.004en
dc.identifier.urihttp://hdl.handle.net/10754/625364-
dc.description.abstractAnaerobic ammonium-oxidation (anammox) is recognized as an important microbial process in the global nitrogen cycle and wastewater treatment. In this study, we successfully enriched a novel anammox bacterium affiliated with the genus ‘Candidatus Brocadia’ with high purity (>90%) in a membrane bioreactor (MBR). The enriched bacterium was distantly related to the hitherto characterized ‘Ca. Brocadia fulgida’ and ‘Ca. Brocadia sinica’ with 96% and 93% of 16S ribosomal RNA gene sequence identity, respectively. The bacterium exhibited the common structural features of anammox bacteria and the production of hydrazine in the presence of hydroxylamine under anoxic conditions. The temperature range of anammox activity was 20 − 45°C with a maximum activity at 37°C. The maximum specific growth rate (μmax) was determined to be 0.0082h−1 at 37°C, corresponding to a doubling time of 3.5 days. The half-saturation constant (KS) for nitrite was 5±2.5μM. The anammox activity was inhibited by nitrite with 11.6mM representing the 50% inhibitory concentration (IC50) but no significant inhibition was observed in the presence of formate and acetate. The major respiratory quinone was identified to be menaquinone-7 (MK-7). Comparative genome analysis revealed that the anammox bacterium enriched in present study shared nearly half of genes with ‘Ca. Brocadia sinica’ and ‘Ca. Brocadia fulgida’. The bacterium enriched in this study showed all known physiological characteristics of anammox bacteria and can be distinguished from the close relatives by its rRNA gene sequences. Therefore, we proposed the name ‘Ca. Brocadia sapporoensis’ sp. nov.en
dc.description.sponsorshipThis research was financially supported by Nagase Science and Technology Foundation and Institute for Fermentation, Osaka (IFO), which were granted to Satoshi Okabe. Lei Zhang was supported partly by the Monbukagakusho Honors Scholarship for Privately Financed International Students offered by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. Post-Doctoral Fellowship of Muhammad Ali was sponsored by Graduate school of Engineering, Hokkaido University, Japan.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0723202017300929en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Systematic and Applied Microbiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Systematic and Applied Microbiology, [, , (2017-08-18)] DOI: 10.1016/j.syapm.2017.07.004 . © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectAnammox enrichment cultureen
dc.subject‘Ca. Brocadia sapporoensis’en
dc.subjectPhysiological characteristicsen
dc.subjectComparative genome analysisen
dc.titleEnrichment and physiological characterization of an anaerobic ammonium-oxidizing bacterium ‘ Candidatus Brocadia sapporoensis’en
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalSystematic and Applied Microbiologyen
dc.eprint.versionPost-printen
dc.contributor.institutionDivision of Environmental Engineering, Faculty of Engineering, Hokkaido University, North-13, West-8, Kita-ku, Sapporo, Hokkaido 060-8628, Japanen
dc.contributor.institutionDepartment of Civil and Environmental Engineering, National Institute of Technology, Kure College, 2-2-11 Agaminami, Kure, Hiroshima 737-8506, Japanen
dc.contributor.institutionDepartment of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, 4-22-1, Ikeda, Nishi-ku, Kumamoto, Kumamoto, 860-0082, Japanen
kaust.authorAli, Muhammaden
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