Temporal Microbial Community Dynamics in Microbial Electrolysis Cells – Influence of Acetate and Propionate Concentration

Handle URI:
http://hdl.handle.net/10754/625289
Title:
Temporal Microbial Community Dynamics in Microbial Electrolysis Cells – Influence of Acetate and Propionate Concentration
Authors:
Rao, Hari Ananda ( 0000-0002-8916-1809 ) ; Venkidusamy, Krishnaveni; Katuri, Krishna; Bagchi, Samik; Saikaly, Pascal ( 0000-0001-7678-3986 )
Abstract:
Microbial electrolysis cells (MECs) are widely considered as a next generation wastewater treatment system. However, fundamental insight on the temporal dynamics of microbial communities associated with MEC performance under different organic types with varied loading concentrations is still unknown, nevertheless this knowledge is essential for optimizing this technology for real-scale applications. Here, the temporal dynamics of anodic microbial communities associated with MEC performance was examined at low (0.5 g COD/L) and high (4 g COD/L) concentrations of acetate or propionate, which are important intermediates of fermentation of municipal wastewaters and sludge. The results showed that acetate-fed reactors exhibited higher performance in terms of maximum current density (I: 4.25 ± 0.23 A/m), coulombic efficiency (CE: 95 ± 8%), and substrate degradation rate (98.8 ± 1.2%) than propionate-fed reactors (I: 2.7 ± 0.28 A/m; CE: 68 ± 9.5%; substrate degradation rate: 84 ± 13%) irrespective of the concentrations tested. Despite of the repeated sampling of the anodic biofilm over time, the high-concentration reactors demonstrated lower and stable performance in terms of current density (I: 1.1 ± 0.14 to 4.2 ± 0.21 A/m), coulombic efficiency (CE: 44 ± 4.1 to 103 ± 7.2%) and substrate degradation rate (64.9 ± 6.3 to 99.7 ± 0.5%), while the low-concentration reactors produced higher and dynamic performance (I: 1.1 ± 0.12 to 4.6 ± 0.1 A/m; CE: 52 ± 2.5 to 105 ± 2.7%; substrate degradation rate: 87.2 ± 0.2 to 99.9 ± 0.06%) with the different substrates tested. Correlating reactor's performance with temporal dynamics of microbial communities showed that relatively similar anodic microbial community composition but with varying relative abundances was observed in all the reactors despite differences in the substrate and concentrations tested. Particularly, Geobacter was the predominant bacteria on the anode biofilm of all MECs over time suggesting its possible role in maintaining functional stability of MECs fed with low and high concentrations of acetate and propionate. Taken together, these results provide new insights on the microbial community dynamics and its correlation to performance in MECs fed with different concentrations of acetate and propionate, which are important volatile fatty acids in wastewater.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Hari AR, Venkidusamy K, Katuri KP, Bagchi S, Saikaly PE (2017) Temporal Microbial Community Dynamics in Microbial Electrolysis Cells – Influence of Acetate and Propionate Concentration. Frontiers in Microbiology 8. Available: http://dx.doi.org/10.3389/fmicb.2017.01371.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Microbiology
Issue Date:
20-Jul-2017
DOI:
10.3389/fmicb.2017.01371
Type:
Article
ISSN:
1664-302X
Sponsors:
This work was sponsored by a Ph.D. fellowship, a Global Research Partnership-Collaborative Fellows Award (GRP-CF-2011-15-S) and Center Competitive Funding (FCC/1/1971–05–01) to PS from King Abdullah University of Science and Technology (KAUST). The authors thank Prof. Bruce E. Logan, Pennsylvania State University, United States for comments and suggestions on previous versions of this manuscript.
Additional Links:
http://journal.frontiersin.org/article/10.3389/fmicb.2017.01371/full
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorRao, Hari Anandaen
dc.contributor.authorVenkidusamy, Krishnavenien
dc.contributor.authorKaturi, Krishnaen
dc.contributor.authorBagchi, Samiken
dc.contributor.authorSaikaly, Pascalen
dc.date.accessioned2017-08-03T11:56:25Z-
dc.date.available2017-08-03T11:56:25Z-
dc.date.issued2017-07-20en
dc.identifier.citationHari AR, Venkidusamy K, Katuri KP, Bagchi S, Saikaly PE (2017) Temporal Microbial Community Dynamics in Microbial Electrolysis Cells – Influence of Acetate and Propionate Concentration. Frontiers in Microbiology 8. Available: http://dx.doi.org/10.3389/fmicb.2017.01371.en
dc.identifier.issn1664-302Xen
dc.identifier.doi10.3389/fmicb.2017.01371en
dc.identifier.urihttp://hdl.handle.net/10754/625289-
dc.description.abstractMicrobial electrolysis cells (MECs) are widely considered as a next generation wastewater treatment system. However, fundamental insight on the temporal dynamics of microbial communities associated with MEC performance under different organic types with varied loading concentrations is still unknown, nevertheless this knowledge is essential for optimizing this technology for real-scale applications. Here, the temporal dynamics of anodic microbial communities associated with MEC performance was examined at low (0.5 g COD/L) and high (4 g COD/L) concentrations of acetate or propionate, which are important intermediates of fermentation of municipal wastewaters and sludge. The results showed that acetate-fed reactors exhibited higher performance in terms of maximum current density (I: 4.25 ± 0.23 A/m), coulombic efficiency (CE: 95 ± 8%), and substrate degradation rate (98.8 ± 1.2%) than propionate-fed reactors (I: 2.7 ± 0.28 A/m; CE: 68 ± 9.5%; substrate degradation rate: 84 ± 13%) irrespective of the concentrations tested. Despite of the repeated sampling of the anodic biofilm over time, the high-concentration reactors demonstrated lower and stable performance in terms of current density (I: 1.1 ± 0.14 to 4.2 ± 0.21 A/m), coulombic efficiency (CE: 44 ± 4.1 to 103 ± 7.2%) and substrate degradation rate (64.9 ± 6.3 to 99.7 ± 0.5%), while the low-concentration reactors produced higher and dynamic performance (I: 1.1 ± 0.12 to 4.6 ± 0.1 A/m; CE: 52 ± 2.5 to 105 ± 2.7%; substrate degradation rate: 87.2 ± 0.2 to 99.9 ± 0.06%) with the different substrates tested. Correlating reactor's performance with temporal dynamics of microbial communities showed that relatively similar anodic microbial community composition but with varying relative abundances was observed in all the reactors despite differences in the substrate and concentrations tested. Particularly, Geobacter was the predominant bacteria on the anode biofilm of all MECs over time suggesting its possible role in maintaining functional stability of MECs fed with low and high concentrations of acetate and propionate. Taken together, these results provide new insights on the microbial community dynamics and its correlation to performance in MECs fed with different concentrations of acetate and propionate, which are important volatile fatty acids in wastewater.en
dc.description.sponsorshipThis work was sponsored by a Ph.D. fellowship, a Global Research Partnership-Collaborative Fellows Award (GRP-CF-2011-15-S) and Center Competitive Funding (FCC/1/1971–05–01) to PS from King Abdullah University of Science and Technology (KAUST). The authors thank Prof. Bruce E. Logan, Pennsylvania State University, United States for comments and suggestions on previous versions of this manuscript.en
dc.publisherFrontiers Media SAen
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmicb.2017.01371/fullen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAcetateen
dc.subjectGeobacteren
dc.subjectMicrobial community dynamicsen
dc.subjectMicrobial electrolysis cellsen
dc.subjectPropionateen
dc.titleTemporal Microbial Community Dynamics in Microbial Electrolysis Cells – Influence of Acetate and Propionate Concentrationen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalFrontiers in Microbiologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionCentre for Environmental Risk Assessment and Remediation, University of South Australia, Mawson Lakes, SA, , Australiaen
dc.contributor.institutionDepartment of Civil, Environmental and Architectural Engineering, University of Kansas, Lawrence, KS, , United Statesen
kaust.authorRao, Hari Anandaen
kaust.authorKaturi, Krishnaen
kaust.authorSaikaly, Pascalen
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