Membrane Bioreactor-Based Wastewater Treatment Plant in Saudi Arabia: Reduction of Viral Diversity, Load, and Infectious Capacity

Handle URI:
http://hdl.handle.net/10754/625255
Title:
Membrane Bioreactor-Based Wastewater Treatment Plant in Saudi Arabia: Reduction of Viral Diversity, Load, and Infectious Capacity
Authors:
Jumat, Muhammad; Hasan, Nur; Subramanian, Poorani; Heberling, Colin; Colwell, Rita; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
A membrane bioreactor (MBR)-based wastewater treatment plant in Saudi Arabia was assessed over a nine-month period for virus removal efficiency. Viral diversity was detected using omics-based approaches. Log reduction values (LRV) of Adenoviruses (AdV) and Enteroviruses (EV) were enumerated using digital polymerase chain reaction (dPCR) and assessed for infectivity using fluorescence-based infection assays. MBR treatment was successful in reducing viral diversity. Plant viruses remained abundant in the treated effluent. Human enteric viruses were present in lower abundance than plant viruses, and were reduced by MBR at varying LRV. AdV copy numbers were reduced by 3.7-log. Infectious AdV was not detected in the effluent. EV copy numbers were reduced by 1.7-log post MBR and infectious EV decreased by an average of 2.0-log. Infectious EV was detected in the chlorinated effluent, occasionally in concentrations that approximate to its 50% infectious dose. Overall, results indicated that a MBR-based wastewater treatment plant (WWTP) effectively reduces viral diversity, viral load, and infectious capacity by up to 4-logs. These findings suggest potential concerns associated with plant and human enteric viruses for reuse events in this country. Local guidelines for assessment of treated water quality should take into consideration both infectious viral concentration and LRV.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Jumat M, Hasan N, Subramanian P, Heberling C, Colwell R, et al. (2017) Membrane Bioreactor-Based Wastewater Treatment Plant in Saudi Arabia: Reduction of Viral Diversity, Load, and Infectious Capacity. Water 9: 534. Available: http://dx.doi.org/10.3390/w9070534.
Publisher:
MDPI AG
Journal:
Water
Issue Date:
18-Jul-2017
DOI:
10.3390/w9070534
Type:
Article
ISSN:
2073-4441
Sponsors:
The authors would like to thank George Princeton Dunsford for access to the KAUST wastewater treatment plant and Moustapha Harb for providing sampling assistance. The research reported in this publication was supported by the KAUST baseline funding BAS/1/1033-01-01 awarded to Pei-Ying Hong.
Additional Links:
http://www.mdpi.com/2073-4441/9/7/534
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorJumat, Muhammaden
dc.contributor.authorHasan, Nuren
dc.contributor.authorSubramanian, Pooranien
dc.contributor.authorHeberling, Colinen
dc.contributor.authorColwell, Ritaen
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2017-07-26T06:19:10Z-
dc.date.available2017-07-26T06:19:10Z-
dc.date.issued2017-07-18en
dc.identifier.citationJumat M, Hasan N, Subramanian P, Heberling C, Colwell R, et al. (2017) Membrane Bioreactor-Based Wastewater Treatment Plant in Saudi Arabia: Reduction of Viral Diversity, Load, and Infectious Capacity. Water 9: 534. Available: http://dx.doi.org/10.3390/w9070534.en
dc.identifier.issn2073-4441en
dc.identifier.doi10.3390/w9070534en
dc.identifier.urihttp://hdl.handle.net/10754/625255-
dc.description.abstractA membrane bioreactor (MBR)-based wastewater treatment plant in Saudi Arabia was assessed over a nine-month period for virus removal efficiency. Viral diversity was detected using omics-based approaches. Log reduction values (LRV) of Adenoviruses (AdV) and Enteroviruses (EV) were enumerated using digital polymerase chain reaction (dPCR) and assessed for infectivity using fluorescence-based infection assays. MBR treatment was successful in reducing viral diversity. Plant viruses remained abundant in the treated effluent. Human enteric viruses were present in lower abundance than plant viruses, and were reduced by MBR at varying LRV. AdV copy numbers were reduced by 3.7-log. Infectious AdV was not detected in the effluent. EV copy numbers were reduced by 1.7-log post MBR and infectious EV decreased by an average of 2.0-log. Infectious EV was detected in the chlorinated effluent, occasionally in concentrations that approximate to its 50% infectious dose. Overall, results indicated that a MBR-based wastewater treatment plant (WWTP) effectively reduces viral diversity, viral load, and infectious capacity by up to 4-logs. These findings suggest potential concerns associated with plant and human enteric viruses for reuse events in this country. Local guidelines for assessment of treated water quality should take into consideration both infectious viral concentration and LRV.en
dc.description.sponsorshipThe authors would like to thank George Princeton Dunsford for access to the KAUST wastewater treatment plant and Moustapha Harb for providing sampling assistance. The research reported in this publication was supported by the KAUST baseline funding BAS/1/1033-01-01 awarded to Pei-Ying Hong.en
dc.publisherMDPI AGen
dc.relation.urlhttp://www.mdpi.com/2073-4441/9/7/534en
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectlog removal valuesen
dc.subjectenterovirusesen
dc.subjectadenovirusesen
dc.subjectdiagnostic immunofluorescenceen
dc.subjectwater reuseen
dc.titleMembrane Bioreactor-Based Wastewater Treatment Plant in Saudi Arabia: Reduction of Viral Diversity, Load, and Infectious Capacityen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalWateren
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionCenter for Bioinformatics and Computational Biology, University of Maryland Institute of Advanced Computer Studies, University of Maryland, College Park, MD 20742, USAen
dc.contributor.institutionCosmosID, Inc., Rockville, MD 20850, USAen
dc.contributor.institutionBloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21205, USAen
kaust.authorJumat, Muhammaden
kaust.authorHong, Pei-Yingen
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