The endophytic fungus Piriformospora indica enhances Arabidopsis thaliana growth and modulates Na + /K + homeostasis under salt stress conditions

Handle URI:
http://hdl.handle.net/10754/625236
Title:
The endophytic fungus Piriformospora indica enhances Arabidopsis thaliana growth and modulates Na + /K + homeostasis under salt stress conditions
Authors:
Abdelaziz, Mohamed Ewis; Kim, Dongjin ( 0000-0003-4175-9817 ) ; Ali, Shawkat ( 0000-0003-3282-3186 ) ; Fedoroff, Nina V.; Al-Babili, Salim ( 0000-0003-4823-2882 )
Abstract:
The mutualistic, endophytic fungus Piriformospora indica has been shown to confer biotic and abiotic stress tolerance to host plants. In this study, we investigated the impact of P. indica on the growth of Arabidopsis plants under normal and salt stress conditions. Our results demonstrate that P. indica colonization increases plant biomass, lateral roots density, and chlorophyll content under both conditions. Colonization with P. indica under salt stress was accompanied by a lower Na+/K+ ratio and less pronounced accumulation of anthocyanin, compared to control plants. Moreover, P. indica colonized roots under salt stress showed enhanced transcript levels of the genes encoding the high Affinity Potassium Transporter 1 (HKT1) and the inward-rectifying K+ channels KAT1 and KAT2, which play key roles in regulating Na+ and K+ homeostasis. The effect of P. indica colonization on AtHKT1;1 expression was also confirmed in the Arabidopsis line gl1-HKT:AtHKT1;1 that expresses an additional AtHKT1;1 copy driven by the native promoter. Colonization of the gl1-HKT:AtHKT1;1 by P. indica also increased lateral roots density and led to a better Na+/K+ ratio, which may be attributed to the observed increase in KAT1 and KAT2 transcript levels. Our findings demonstrate that P. indica colonization promotes Arabidopsis growth under salt stress conditions and that this effect is likely caused by modulation of the expression levels of the major Na+ and K+ ion channels, which allows establishing a balanced ion homeostasis of Na+/K+ under salt stress conditions.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Abdelaziz ME, Kim D, Ali S, Fedoroff NV, Al-Babili S (2017) The endophytic fungus Piriformospora indica enhances Arabidopsis thaliana growth and modulates Na + /K + homeostasis under salt stress conditions. Plant Science. Available: http://dx.doi.org/10.1016/j.plantsci.2017.07.006.
Publisher:
Elsevier BV
Journal:
Plant Science
Issue Date:
13-Jul-2017
DOI:
10.1016/j.plantsci.2017.07.006
Type:
Article
ISSN:
0168-9452
Sponsors:
We would like to thank Dr. Heribert Hirt and Dr. Zahir Ali for valuable discussions and Dr. Paul M Hasegawa (Purdue University) for providing gl1-HKT:AtHKT1;1 seeds. The research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0168945217304028
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorAbdelaziz, Mohamed Ewisen
dc.contributor.authorKim, Dongjinen
dc.contributor.authorAli, Shawkaten
dc.contributor.authorFedoroff, Nina V.en
dc.contributor.authorAl-Babili, Salimen
dc.date.accessioned2017-07-19T10:45:06Z-
dc.date.available2017-07-19T10:45:06Z-
dc.date.issued2017-07-13en
dc.identifier.citationAbdelaziz ME, Kim D, Ali S, Fedoroff NV, Al-Babili S (2017) The endophytic fungus Piriformospora indica enhances Arabidopsis thaliana growth and modulates Na + /K + homeostasis under salt stress conditions. Plant Science. Available: http://dx.doi.org/10.1016/j.plantsci.2017.07.006.en
dc.identifier.issn0168-9452en
dc.identifier.doi10.1016/j.plantsci.2017.07.006en
dc.identifier.urihttp://hdl.handle.net/10754/625236-
dc.description.abstractThe mutualistic, endophytic fungus Piriformospora indica has been shown to confer biotic and abiotic stress tolerance to host plants. In this study, we investigated the impact of P. indica on the growth of Arabidopsis plants under normal and salt stress conditions. Our results demonstrate that P. indica colonization increases plant biomass, lateral roots density, and chlorophyll content under both conditions. Colonization with P. indica under salt stress was accompanied by a lower Na+/K+ ratio and less pronounced accumulation of anthocyanin, compared to control plants. Moreover, P. indica colonized roots under salt stress showed enhanced transcript levels of the genes encoding the high Affinity Potassium Transporter 1 (HKT1) and the inward-rectifying K+ channels KAT1 and KAT2, which play key roles in regulating Na+ and K+ homeostasis. The effect of P. indica colonization on AtHKT1;1 expression was also confirmed in the Arabidopsis line gl1-HKT:AtHKT1;1 that expresses an additional AtHKT1;1 copy driven by the native promoter. Colonization of the gl1-HKT:AtHKT1;1 by P. indica also increased lateral roots density and led to a better Na+/K+ ratio, which may be attributed to the observed increase in KAT1 and KAT2 transcript levels. Our findings demonstrate that P. indica colonization promotes Arabidopsis growth under salt stress conditions and that this effect is likely caused by modulation of the expression levels of the major Na+ and K+ ion channels, which allows establishing a balanced ion homeostasis of Na+/K+ under salt stress conditions.en
dc.description.sponsorshipWe would like to thank Dr. Heribert Hirt and Dr. Zahir Ali for valuable discussions and Dr. Paul M Hasegawa (Purdue University) for providing gl1-HKT:AtHKT1;1 seeds. The research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST).en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0168945217304028en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Plant Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Plant Science, 13 July 2017. DOI: 10.1016/j.plantsci.2017.07.006. © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.titleThe endophytic fungus Piriformospora indica enhances Arabidopsis thaliana growth and modulates Na + /K + homeostasis under salt stress conditionsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPlant Scienceen
dc.eprint.versionPost-printen
dc.contributor.institutionFaculty of Agriculture, Cairo University, 12613, Giza, Egypten
dc.contributor.institutionDepartment of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717, USAen
kaust.authorAbdelaziz, Mohamed Ewisen
kaust.authorKim, Dongjinen
kaust.authorAli, Shawkaten
kaust.authorFedoroff, Nina V.en
kaust.authorAl-Babili, Salimen
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