NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato

Handle URI:
http://hdl.handle.net/10754/625184
Title:
NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato
Authors:
Thirumalaikumar, Venkatesh P.; Devkar, Vikas; Mehterov, Nikolay; Ali, Shawkat ( 0000-0003-3282-3186 ) ; Ozgur, Rengin; Turkan, Ismail; Mueller-Roeber, Bernd; Balazadeh, Salma
Abstract:
Water deficit (drought stress) massively restricts plant growth and the yield of crops; reducing the deleterious effects of drought is therefore of high agricultural relevance. Drought triggers diverse cellular processes including the inhibition of photosynthesis, the accumulation of cell-damaging reactive oxygen species, and gene expression reprogramming, besides others. Transcription factors (TF) are central regulators of transcriptional reprogramming and expression of many TF genes is affected by drought, including members of the NAC family. Here, we identify the NAC factor JUNGBRUNNEN1 (JUB1) as a regulator of drought tolerance in tomato (Solanum lycopersicum). Expression of tomato JUB1 (SlJUB1) is enhanced by various abiotic stresses, including drought. Inhibiting SlJUB1 by virus-induced gene silencing drastically lowers drought tolerance concomitant with an increase in ion leakage, an elevation of hydrogen peroxide (H2 O2 ) levels, and a decrease of the expression of various drought-responsive genes. In contrast, overexpression of AtJUB1 from Arabidopsis thaliana increases drought tolerance in tomato, alongside with a higher relative leaf water content during drought and reduced H2 O2 levels. AtJUB1 was previously shown to stimulate expression of DREB2A, a TF involved in drought responses, and of the DELLA genes GAI and RGL1. We show here that SlJUB1 similarly controls the expression of the tomato orthologs SlDREB1, SlDREB2, and SlDELLA. Furthermore, AtJUB1 directly binds to the promoters of SlDREB1, SlDREB2 and SlDELLA in tomato. Our study highlights JUB1 as a transcriptional regulator of drought tolerance and suggests considerable conservation of the abiotic stress-related gene regulatory networks controlled by this NAC factor between Arabidopsis and tomato. This article is protected by copyright. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture
Citation:
Thirumalaikumar VP, Devkar V, Mehterov N, Ali S, Ozgur R, et al. (2017) NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato. Plant Biotechnology Journal. Available: http://dx.doi.org/10.1111/pbi.12776.
Publisher:
Wiley-Blackwell
Journal:
Plant Biotechnology Journal
Issue Date:
22-Jun-2017
DOI:
10.1111/pbi.12776
Type:
Article
ISSN:
1467-7644
Sponsors:
This work was supported by funding from the Deutsche Forschungsgemeinschaft (DFG) to S.B. (BA 4769/2-1). Financial support was furthermore provided by the University of Potsdam and the MPI of Molecular Plant Physiology.
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/pbi.12776/abstract
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorThirumalaikumar, Venkatesh P.en
dc.contributor.authorDevkar, Vikasen
dc.contributor.authorMehterov, Nikolayen
dc.contributor.authorAli, Shawkaten
dc.contributor.authorOzgur, Renginen
dc.contributor.authorTurkan, Ismailen
dc.contributor.authorMueller-Roeber, Bernden
dc.contributor.authorBalazadeh, Salmaen
dc.date.accessioned2017-07-12T07:20:55Z-
dc.date.available2017-07-12T07:20:55Z-
dc.date.issued2017-06-22en
dc.identifier.citationThirumalaikumar VP, Devkar V, Mehterov N, Ali S, Ozgur R, et al. (2017) NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato. Plant Biotechnology Journal. Available: http://dx.doi.org/10.1111/pbi.12776.en
dc.identifier.issn1467-7644en
dc.identifier.doi10.1111/pbi.12776en
dc.identifier.urihttp://hdl.handle.net/10754/625184-
dc.description.abstractWater deficit (drought stress) massively restricts plant growth and the yield of crops; reducing the deleterious effects of drought is therefore of high agricultural relevance. Drought triggers diverse cellular processes including the inhibition of photosynthesis, the accumulation of cell-damaging reactive oxygen species, and gene expression reprogramming, besides others. Transcription factors (TF) are central regulators of transcriptional reprogramming and expression of many TF genes is affected by drought, including members of the NAC family. Here, we identify the NAC factor JUNGBRUNNEN1 (JUB1) as a regulator of drought tolerance in tomato (Solanum lycopersicum). Expression of tomato JUB1 (SlJUB1) is enhanced by various abiotic stresses, including drought. Inhibiting SlJUB1 by virus-induced gene silencing drastically lowers drought tolerance concomitant with an increase in ion leakage, an elevation of hydrogen peroxide (H2 O2 ) levels, and a decrease of the expression of various drought-responsive genes. In contrast, overexpression of AtJUB1 from Arabidopsis thaliana increases drought tolerance in tomato, alongside with a higher relative leaf water content during drought and reduced H2 O2 levels. AtJUB1 was previously shown to stimulate expression of DREB2A, a TF involved in drought responses, and of the DELLA genes GAI and RGL1. We show here that SlJUB1 similarly controls the expression of the tomato orthologs SlDREB1, SlDREB2, and SlDELLA. Furthermore, AtJUB1 directly binds to the promoters of SlDREB1, SlDREB2 and SlDELLA in tomato. Our study highlights JUB1 as a transcriptional regulator of drought tolerance and suggests considerable conservation of the abiotic stress-related gene regulatory networks controlled by this NAC factor between Arabidopsis and tomato. This article is protected by copyright. All rights reserved.en
dc.description.sponsorshipThis work was supported by funding from the Deutsche Forschungsgemeinschaft (DFG) to S.B. (BA 4769/2-1). Financial support was furthermore provided by the University of Potsdam and the MPI of Molecular Plant Physiology.en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/pbi.12776/abstracten
dc.rightsThis is the peer reviewed version of the following article: NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato, which has been published in final form at http://doi.org/10.1111/pbi.12776. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.subjectArabidopsisen
dc.subjectReactive oxygen speciesen
dc.subjectTranscription factoren
dc.subjectTomatoen
dc.subjectDroughten
dc.subjectDellaen
dc.titleNAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomatoen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalPlant Biotechnology Journalen
dc.eprint.versionPost-printen
dc.contributor.institutionUniversity of Potsdam; Institute of Biochemistry and Biology; Karl-Liebknecht-Straße 24-25, Haus 20 14476 Potsdam-Golm Germanyen
dc.contributor.institutionMax Planck Institute of Molecular Plant Physiology; Am Mühlenberg 1 14476 Potsdam-Golm Germanyen
dc.contributor.institutionEge University; Faculty of Science, Department of Biology; TR-35100 Izmir Turkeyen
kaust.authorAli, Shawkaten
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