Colonization by the endophyte Piriformospora indica leads to early flowering in Arabidopsis thaliana likely by triggering gibberellin biosynthesis

Handle URI:
http://hdl.handle.net/10754/625159
Title:
Colonization by the endophyte Piriformospora indica leads to early flowering in Arabidopsis thaliana likely by triggering gibberellin biosynthesis
Authors:
Kim, Dongjin ( 0000-0003-4175-9817 ) ; Abdelaziz, Mohamad E.; Ntui, Valentine Otang; Guo, Xiujie; Al-Babili, Salim ( 0000-0003-4823-2882 )
Abstract:
Piriformospora indica is an endophytic fungus colonizing roots of a wide variety of plants. Previous studies showed that P. indica promotes early flowering and plant growth in the medicinal plant Coleus forskohlii. To determine the impact of P. indica on flowering time in Arabidopsis, we co-cultivated the plants with P. indica under long day condition. P. indica inoculated Arabidopsis plants displayed significant early flowering phenotype. qRT-PCR analysis of colonized plants revealed an up-regulation of flowering regulatory (FLOWERING LOCUS T, LEAFY, and APETALA1) and gibberellin biosynthetic (Gibberellin 20-Oxidase2, Gibberellin 3-Oxidase1 and Gibberellin requiring1) genes, while the flowering-repressing gene FLOWERING LOCUS C was down regulated. Quantification of gibberellins content showed that the colonization with P. indica caused an increase in GA4 content. Compared to wild-type plants, inoculation of the Arabidopsis ga5 mutant affected in gibberellin biosynthetic gene led to less pronounced changes in the expression of genes regulating flowering and to a lower increase in GA4 content. Taken together, our data indicate that P. indica promotes early flowering in Arabidopsis likely by increasing gibberellin content.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Kim D, Abdelaziz ME, Ntui VO, Guo X, Al-Babili S (2017) Colonization by the endophyte Piriformospora indica leads to early flowering in Arabidopsis thaliana likely by triggering gibberellin biosynthesis. Biochemical and Biophysical Research Communications. Available: http://dx.doi.org/10.1016/j.bbrc.2017.06.169.
Publisher:
Elsevier BV
Journal:
Biochemical and Biophysical Research Communications
Issue Date:
28-Jun-2017
DOI:
10.1016/j.bbrc.2017.06.169
Type:
Article
ISSN:
0006-291X
Sponsors:
This work was funded by King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0006291X17313013
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorKim, Dongjinen
dc.contributor.authorAbdelaziz, Mohamad E.en
dc.contributor.authorNtui, Valentine Otangen
dc.contributor.authorGuo, Xiujieen
dc.contributor.authorAl-Babili, Salimen
dc.date.accessioned2017-07-06T09:43:05Z-
dc.date.available2017-07-06T09:43:05Z-
dc.date.issued2017-06-28en
dc.identifier.citationKim D, Abdelaziz ME, Ntui VO, Guo X, Al-Babili S (2017) Colonization by the endophyte Piriformospora indica leads to early flowering in Arabidopsis thaliana likely by triggering gibberellin biosynthesis. Biochemical and Biophysical Research Communications. Available: http://dx.doi.org/10.1016/j.bbrc.2017.06.169.en
dc.identifier.issn0006-291Xen
dc.identifier.doi10.1016/j.bbrc.2017.06.169en
dc.identifier.urihttp://hdl.handle.net/10754/625159-
dc.description.abstractPiriformospora indica is an endophytic fungus colonizing roots of a wide variety of plants. Previous studies showed that P. indica promotes early flowering and plant growth in the medicinal plant Coleus forskohlii. To determine the impact of P. indica on flowering time in Arabidopsis, we co-cultivated the plants with P. indica under long day condition. P. indica inoculated Arabidopsis plants displayed significant early flowering phenotype. qRT-PCR analysis of colonized plants revealed an up-regulation of flowering regulatory (FLOWERING LOCUS T, LEAFY, and APETALA1) and gibberellin biosynthetic (Gibberellin 20-Oxidase2, Gibberellin 3-Oxidase1 and Gibberellin requiring1) genes, while the flowering-repressing gene FLOWERING LOCUS C was down regulated. Quantification of gibberellins content showed that the colonization with P. indica caused an increase in GA4 content. Compared to wild-type plants, inoculation of the Arabidopsis ga5 mutant affected in gibberellin biosynthetic gene led to less pronounced changes in the expression of genes regulating flowering and to a lower increase in GA4 content. Taken together, our data indicate that P. indica promotes early flowering in Arabidopsis likely by increasing gibberellin content.en
dc.description.sponsorshipThis work was funded by King Abdullah University of Science and Technology (KAUST).en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X17313013en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications, [, , (2017-06-28)] DOI: 10.1016/j.bbrc.2017.06.169 . © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectGibberellinen
dc.subjectEndophytic fungusen
dc.subjectPiriformospora indicaen
dc.subjectEarly Floweringen
dc.titleColonization by the endophyte Piriformospora indica leads to early flowering in Arabidopsis thaliana likely by triggering gibberellin biosynthesisen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalBiochemical and Biophysical Research Communicationsen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Plant Sciences and Plant Pathology, Montana State University, USA.en
dc.contributor.institutionFaculty of Agriculture, Cairo University, Giza 12613, Egypt.en
dc.contributor.institutionInternational Institute of Tropical Agriculture, Nairobi, Kenya.en
kaust.authorKim, Dongjinen
kaust.authorAbdelaziz, Mohamad E.en
kaust.authorNtui, Valentine Otangen
kaust.authorGuo, Xiujieen
kaust.authorAl-Babili, Salimen
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